JoVE Encyclopedia of Experiments

Immunology

Video encyclopedias of advanced research experiments for scientists in academia and biotech

Start free trial

1,250+ Videos

Filmed at top university labs

Practice and theory for each experiment

Table of Contents

Immune Systems and Components

45 Videos

194 Minutes

Cell Isolation and Culture

20 Videos

82 Minutes

Isolation of Monocytes from Whole Blood by Immunomagnetic Negative Selection
4:27

Isolation of Monocytes from Whole Blood by Immunomagnetic Negative Selection

1.7K views

Isolation and Culture of Mouse Kupffer Cells
3:53

Isolation and Culture of Mouse Kupffer Cells

3.5K views

Isolation of Macrophages from Mouse Dorsal Root Ganglion Using Mechanical Dissociation
6:56

Isolation of Macrophages from Mouse Dorsal Root Ganglion Using Mechanical Dissociation

926 views

Isolation of Lymphocytes from a Mouse Peyer's Patch
6:02

Isolation of Lymphocytes from a Mouse Peyer's Patch

1.7K views

Isolation of Peripheral Blood Mononuclear Cells from Human Buffy Coats
2:45

Isolation of Peripheral Blood Mononuclear Cells from Human Buffy Coats

2.9K views

Isolation and Purification of B Cells from Human Peripheral Blood
3:58

Isolation and Purification of B Cells from Human Peripheral Blood

691 views

Isolation and Culture of Bone Marrow Stromal Cells from a Mouse Tibia
4:00

Isolation and Culture of Bone Marrow Stromal Cells from a Mouse Tibia

1.4K views

Flotation-Based T Cell Isolation Using Buoyancy-Activated Cell Sorting
3:18

Flotation-Based T Cell Isolation Using Buoyancy-Activated Cell Sorting

658 views

Culturing of Activated T Cells from Human Peripheral Blood Mononuclear Cells
3:25

Culturing of Activated T Cells from Human Peripheral Blood Mononuclear Cells

918 views

Isolation of Marginating Hepatic Leukocytes from a Mouse Liver
3:42

Isolation of Marginating Hepatic Leukocytes from a Mouse Liver

465 views

Natural Killer Cell Isolation from Liver Tissue for Selective Expansion
4:17

Natural Killer Cell Isolation from Liver Tissue for Selective Expansion

587 views

Ex Vivo Expansion of Natural Killer Cells from Peripheral Blood Mononuclear Cells
3:14

Ex Vivo Expansion of Natural Killer Cells from Peripheral Blood Mononuclear Cells

767 views

Isolation of Immune Regulatory Sertoli Cells from Mouse Seminiferous Tubules
4:25

Isolation of Immune Regulatory Sertoli Cells from Mouse Seminiferous Tubules

686 views

Selective Isolation of  CD4<sup>+</sup> Vδ1<sup>+</sup> γδ T Cells from Human Peripheral Blood Mononuclear Cells
6:39

Selective Isolation of CD4+ Vδ1+ γδ T Cells from Human Peripheral Blood Mononuclear Cells

435 views

Isolation of Cytotoxic Neutrophils from Tumor-Bearing Mouse Blood
4:21

Isolation of Cytotoxic Neutrophils from Tumor-Bearing Mouse Blood

580 views

Isolation of Gingival Immune Cell Network from Murine Teeth Blocks
3:19

Isolation of Gingival Immune Cell Network from Murine Teeth Blocks

549 views

A Technique to Isolate Primary Mast Cells from a Murine Peritoneal Cavity
4:17

A Technique to Isolate Primary Mast Cells from a Murine Peritoneal Cavity

802 views

Magnetic Isolation of Microglia from Mouse Pup Brains
4:45

Magnetic Isolation of Microglia from Mouse Pup Brains

623 views

Harvesting an Inguinal Draining Lymph Node from a Murine Model
2:29

Harvesting an Inguinal Draining Lymph Node from a Murine Model

3.4K views

A Dissection Method to Harvest Murine Popliteal Draining Lymph Nodes for Ex Vivo Imaging
2:16

A Dissection Method to Harvest Murine Popliteal Draining Lymph Nodes for Ex Vivo Imaging

2.6K views

Immune Response

45 Videos

176 Minutes

Host Defense Mechanism

24 Videos

95 Minutes

Monitoring Neutrophil Recruitment Dynamics and Bacterial Burden at the Infection Site in a Murine Model
4:29

Monitoring Neutrophil Recruitment Dynamics and Bacterial Burden at the Infection Site in a Murine Model

756 views

Activation of Mouse Bone Marrow-Derived Macrophages in Response to Antibodies
6:51

Activation of Mouse Bone Marrow-Derived Macrophages in Response to Antibodies

505 views

In Vivo Activation of Peritoneal Macrophages in Response to Antibodies in a Murine Model
5:05

In Vivo Activation of Peritoneal Macrophages in Response to Antibodies in a Murine Model

585 views

An Ex Vivo Technique to Induce Neuronal Death via Oligodendrocyte-Specific CD8+ T Cells
3:50

An Ex Vivo Technique to Induce Neuronal Death via Oligodendrocyte-Specific CD8+ T Cells

376 views

Evaluation of Vaccine-Induced Immunity Against Bacterial Infection in a Mouse Model
8:18

Evaluation of Vaccine-Induced Immunity Against Bacterial Infection in a Mouse Model

585 views

Organelle Dynamics in B Lymphocytes following Activation with Antigen-Coated Beads
3:24

Organelle Dynamics in B Lymphocytes following Activation with Antigen-Coated Beads

352 views

B Cell Activation on an Antigen-Coated Coverslip
2:41

B Cell Activation on an Antigen-Coated Coverslip

569 views

A Technique to Generate a Murine Model of Dextran Sulfate Sodium-Induced Colitis
3:20

A Technique to Generate a Murine Model of Dextran Sulfate Sodium-Induced Colitis

601 views

Effect of the Anti C-fms Antibody on Osteoclastogenesis in Mouse Bone Marrow Cells In Vitro
4:27

Effect of the Anti C-fms Antibody on Osteoclastogenesis in Mouse Bone Marrow Cells In Vitro

310 views

Quantification of Inflammatory Mediators in Infected Human Tissue-Derived Cells
3:45

Quantification of Inflammatory Mediators in Infected Human Tissue-Derived Cells

409 views

Determination of ROS Production by Neutrophils upon Interaction with Opsonized Biofilm
3:47

Determination of ROS Production by Neutrophils upon Interaction with Opsonized Biofilm

421 views

An In Vitro Assay to Study the Interaction between Neutrophils and Biofilm
3:06

An In Vitro Assay to Study the Interaction between Neutrophils and Biofilm

365 views

Detection of Inflammasome Activation via Fluorescence Microscopy
3:44

Detection of Inflammasome Activation via Fluorescence Microscopy

441 views

An In Vitro Technique to Study Inflammasome Complex Formation in Macrophages
3:06

An In Vitro Technique to Study Inflammasome Complex Formation in Macrophages

369 views

A Lactate Dehydrogenase Release Assay To Detect Macrophage Death Following Nigericin Exposure
3:02

A Lactate Dehydrogenase Release Assay To Detect Macrophage Death Following Nigericin Exposure

494 views

Induction and Visualization of Neutrophil Extracellular Traps in Pre-Labeled Neutrophils
4:32

Induction and Visualization of Neutrophil Extracellular Traps in Pre-Labeled Neutrophils

571 views

Isolation of Lipids from Human Blood-Derived Neutrophils by Biphasic Separation
2:57

Isolation of Lipids from Human Blood-Derived Neutrophils by Biphasic Separation

390 views

A Lactate Dehydrogenase Release Assay to Detect <em>Cryptococcus neoformans</em> Infection in Macrophages
3:25

A Lactate Dehydrogenase Release Assay to Detect Cryptococcus neoformans Infection in Macrophages

401 views

A Permeable Membrane Insert-Based Infection System to Study the Impact of Bacterial Toxins
3:28

A Permeable Membrane Insert-Based Infection System to Study the Impact of Bacterial Toxins

339 views

Extracting Host Cell Lysate from Infected Host Cells Cultured in a Permeable Membrane System
4:15

Extracting Host Cell Lysate from Infected Host Cells Cultured in a Permeable Membrane System

303 views

Detection of Bacterial Adherence to Host Cells Using Fluorescence Imaging
3:05

Detection of Bacterial Adherence to Host Cells Using Fluorescence Imaging

431 views

An Imaging Technique to Study the Calcium Response in Bacteria-Infected Cells
3:43

An Imaging Technique to Study the Calcium Response in Bacteria-Infected Cells

342 views

Analysis of Immune Synapses between Human T Cells and SEB-Loaded Raji Cells using Imaging Flow Cytometry
4:28

Analysis of Immune Synapses between Human T Cells and SEB-Loaded Raji Cells using Imaging Flow Cytometry

402 views

An Assay to Study the Regulation of Macrophage Phagocytosis by Mesenchymal Stem Cells
3:00

An Assay to Study the Regulation of Macrophage Phagocytosis by Mesenchymal Stem Cells

493 views

Immunopathology

46 Videos

154 Minutes

Host-Pathogen Interaction Models

22 Videos

78 Minutes

A Technique to Induce Chronic Experimental Autoimmune Dry Eye in Rats
1:39

A Technique to Induce Chronic Experimental Autoimmune Dry Eye in Rats

241 views

<em>Malassezia</em>-Induced Skin Inflammation and Fungal Burden Analysis in a Murine Model
3:46

Malassezia-Induced Skin Inflammation and Fungal Burden Analysis in a Murine Model

639 views

A Murine Skin Wound Infection Model to Study the Immune Response against a Bacterial Pathogen
4:00

A Murine Skin Wound Infection Model to Study the Immune Response against a Bacterial Pathogen

648 views

A Human Cervical Mucosa Infection Model to Study HIV Pathogenesis in Ex Vivo Conditions
4:51

A Human Cervical Mucosa Infection Model to Study HIV Pathogenesis in Ex Vivo Conditions

446 views

Intravital Microscopy to Analyze Blood Cell-Endothelial Interactions in an Inflammation Mouse Model
4:45

Intravital Microscopy to Analyze Blood Cell-Endothelial Interactions in an Inflammation Mouse Model

555 views

Subcutaneous Injection of Bacterial Antigen to Induce Systemic Inflammation in a Murine Model
3:52

Subcutaneous Injection of Bacterial Antigen to Induce Systemic Inflammation in a Murine Model

791 views

A Murine Model of Primed Mycobacterial Uveitis to Study Post-Infectious Uveitis
4:00

A Murine Model of Primed Mycobacterial Uveitis to Study Post-Infectious Uveitis

417 views

A Technique to Establish a Bacterial Infection Model in Insect Larvae
3:54

A Technique to Establish a Bacterial Infection Model in Insect Larvae

532 views

Measurement of the In Vivo Burden of Bacteria Expressing Luciferase in an Insect Larva
3:23

Measurement of the In Vivo Burden of Bacteria Expressing Luciferase in an Insect Larva

323 views

An Air Pouch Mouse Model for Lipopolysaccharide-Induced Inflammatory Exudate Collection
3:41

An Air Pouch Mouse Model for Lipopolysaccharide-Induced Inflammatory Exudate Collection

1.3K views

A Technique to Generate an Adjuvant-Induced Arthritis Mouse Model
3:29

A Technique to Generate an Adjuvant-Induced Arthritis Mouse Model

1.6K views

An Intragastric Gavage Technique for Controlled <em>Helicobacter</em> Infection in Mice
3:04

An Intragastric Gavage Technique for Controlled Helicobacter Infection in Mice

998 views

Transurethral Catheterization and Bacterial Inoculation to Generate a Urinary Tract Infection Model in Male Mice
2:32

Transurethral Catheterization and Bacterial Inoculation to Generate a Urinary Tract Infection Model in Male Mice

502 views

Establishment of a Urinary Tract Infection Model in Female Mice
2:54

Establishment of a Urinary Tract Infection Model in Female Mice

695 views

Induction of Graft Versus Host Disease in Mouse Models via Allogeneic Bone Marrow and Splenic Cell Transplantation
5:54

Induction of Graft Versus Host Disease in Mouse Models via Allogeneic Bone Marrow and Splenic Cell Transplantation

442 views

Generation of a Mouse Model of Experimental Cerebral Malaria Using Host Mosquito-Derived Sporozoites
3:28

Generation of a Mouse Model of Experimental Cerebral Malaria Using Host Mosquito-Derived Sporozoites

357 views

A Spray Inoculation Technique to Establish Fungal Infection in Rice Plant Leaves
3:30

A Spray Inoculation Technique to Establish Fungal Infection in Rice Plant Leaves

520 views

Establishment of Rice Plant Leaf Infection through Fungal Inoculation via Wounding
2:26

Establishment of Rice Plant Leaf Infection through Fungal Inoculation via Wounding

495 views

Intrabronchial Instillation of <em>Streptococcus pneumoniae</em> in Immunocompetent Rodent Models
4:46

Intrabronchial Instillation of Streptococcus pneumoniae in Immunocompetent Rodent Models

329 views

Establishing a Mouse Model of Pleural Disseminated Lung Cancer
2:36

Establishing a Mouse Model of Pleural Disseminated Lung Cancer

675 views

A Technique to Induce Chronic Experimental Autoimmune Dry Eye in Rats
3:03

A Technique to Induce Chronic Experimental Autoimmune Dry Eye in Rats

0 views

Induction of Experimental Autoimmune Encephalomyelitis in a Mouse Model
2:45

Induction of Experimental Autoimmune Encephalomyelitis in a Mouse Model

668 views

Assay and Analysis

15 Videos

47 Minutes

Immunodiagnostics

162 Videos

683 Minutes

Cellular Immunodiagnostics

46 Videos

192 Minutes

Analysis of Efferocytosis of Apoptotic Thymocytes by Peritoneal Macrophages
5:36

Analysis of Efferocytosis of Apoptotic Thymocytes by Peritoneal Macrophages

871 views

Flow Cytometry Staining of <em>Mycobacterium tuberculosis</em> Infected Macrophage Subpopulations
3:18

Flow Cytometry Staining of Mycobacterium tuberculosis Infected Macrophage Subpopulations

531 views

Bacterial Cell Staining and Preparation for Immunological Studies
2:57

Bacterial Cell Staining and Preparation for Immunological Studies

531 views

Detection of Vitronectin Binding to Bacterial Surfaces using Flow Cytometry
3:05

Detection of Vitronectin Binding to Bacterial Surfaces using Flow Cytometry

353 views

Evaluation of Stromal Cytokine-Induced Intracellular Protein Phosphorylation Using Phospho-Flow Cytometry
3:40

Evaluation of Stromal Cytokine-Induced Intracellular Protein Phosphorylation Using Phospho-Flow Cytometry

407 views

Analyzing Basophil Activation in the Presence of a Test Allergen using Flow Cytometry
5:37

Analyzing Basophil Activation in the Presence of a Test Allergen using Flow Cytometry

427 views

A Technique for Magnetic Glass Particle-Mediated Nucleic Acid Extraction from Blood
3:43

A Technique for Magnetic Glass Particle-Mediated Nucleic Acid Extraction from Blood

539 views

Measuring Mast Cell Exocytosis via Neuropeptide Y Monomeric Red Fluorescent Protein Release
3:14

Measuring Mast Cell Exocytosis via Neuropeptide Y Monomeric Red Fluorescent Protein Release

280 views

Real-Time Monitoring of Energy Metabolism in Human NK Cells Using an Extracellular Flux Analyzer
4:41

Real-Time Monitoring of Energy Metabolism in Human NK Cells Using an Extracellular Flux Analyzer

362 views

Natural Killer Cell-Mediated Cytotoxicity Assay Sample Preparation for Flow Cytometric Analysis
2:40

Natural Killer Cell-Mediated Cytotoxicity Assay Sample Preparation for Flow Cytometric Analysis

466 views

Detection of Rhesus Factor in Human Blood Samples using Magnetic Levitation
4:10

Detection of Rhesus Factor in Human Blood Samples using Magnetic Levitation

360 views

An In Vitro Technique to Study T Cell Interaction with Supported Planar Lipid Bilayers
5:29

An In Vitro Technique to Study T Cell Interaction with Supported Planar Lipid Bilayers

403 views

Effect of an Immunomodulatory Pharmacological Agent on the Interaction Between Dendritic Cells and CD4+ T Cells
2:40

Effect of an Immunomodulatory Pharmacological Agent on the Interaction Between Dendritic Cells and CD4+ T Cells

348 views

Evaluation of Controlled T Cell Activation with a Photoactivatable Peptide MHC
4:54

Evaluation of Controlled T Cell Activation with a Photoactivatable Peptide MHC

351 views

A Technique to Detect Extrapulmonary Tuberculosis via Superparamagnetic Nanoprobes
2:59

A Technique to Detect Extrapulmonary Tuberculosis via Superparamagnetic Nanoprobes

329 views

Monitoring the Arrival of GFP-Tagged Surface Proteins to the Trans-Golgi Network Using Functionalized Nanobodies
5:00

Monitoring the Arrival of GFP-Tagged Surface Proteins to the Trans-Golgi Network Using Functionalized Nanobodies

267 views

A Microfluidic Chip-Based Method for Rapid Neutrophil Chemotaxis Analysis Using Whole Blood
3:54

A Microfluidic Chip-Based Method for Rapid Neutrophil Chemotaxis Analysis Using Whole Blood

379 views

Assessment of the Activation of Multiple Caspases in Macrophages
4:01

Assessment of the Activation of Multiple Caspases in Macrophages

323 views

A Multiplex Bead-Based Assay for Analyzing Cytokines in Human Tear Samples
4:46

A Multiplex Bead-Based Assay for Analyzing Cytokines in Human Tear Samples

404 views

Measuring Alloreactivity in a Mixed Population of T Cells
5:13

Measuring Alloreactivity in a Mixed Population of T Cells

283 views

Identification and Enumeration of Immune Cells in Bronchoalveolar Lavage Fluid by Flow Cytometry
3:03

Identification and Enumeration of Immune Cells in Bronchoalveolar Lavage Fluid by Flow Cytometry

787 views

A Peptide-MHC-I Tetramer Staining Technique to Analyze SIV-Specific CD8<sup>+</sup> Memory T Cells
4:25

A Peptide-MHC-I Tetramer Staining Technique to Analyze SIV-Specific CD8+ Memory T Cells

405 views

A Laminar Flow-Based Assay to Study the Interactions Between Leukocytes and Endothelial Cells
3:54

A Laminar Flow-Based Assay to Study the Interactions Between Leukocytes and Endothelial Cells

401 views

Quantifying Bulk Autophagic Sequestration Activity in Mammalian Cells
4:33

Quantifying Bulk Autophagic Sequestration Activity in Mammalian Cells

240 views

An Assay to Screen Bioactive Nanoparticles for Toll-Like Receptor Signaling Inhibition
3:53

An Assay to Screen Bioactive Nanoparticles for Toll-Like Receptor Signaling Inhibition

319 views

The Effects of Shear Stress on Platelets Treated with Antibodies Against Mechanosensory Receptors
3:11

The Effects of Shear Stress on Platelets Treated with Antibodies Against Mechanosensory Receptors

317 views

Measuring TEER in X-ray-Irradiated Epithelial Cells Co-cultured With PBMCs
2:17

Measuring TEER in X-ray-Irradiated Epithelial Cells Co-cultured With PBMCs

450 views

An In Vitro Fluorescence-Based Assay to Measure Plasma Membrane Resealing Efficiency
4:34

An In Vitro Fluorescence-Based Assay to Measure Plasma Membrane Resealing Efficiency

331 views

An Immunohistochemistry Staining for the Detection of Rabies Virus Antigens
4:34

An Immunohistochemistry Staining for the Detection of Rabies Virus Antigens

379 views

Immunohistochemistry to Study the Relationship Between Macrophages and Infiltrated Immune Cells
4:00

Immunohistochemistry to Study the Relationship Between Macrophages and Infiltrated Immune Cells

359 views

An Enhanced Crosslinking Immunoprecipitation Method for Efficient Identification of Protein-bound RNA in Mouse Testes
5:07

An Enhanced Crosslinking Immunoprecipitation Method for Efficient Identification of Protein-bound RNA in Mouse Testes

337 views

An Assay for Cell Cycle Analysis of Antigen-Specific CD8<sup>+</sup> T Cells
6:20

An Assay for Cell Cycle Analysis of Antigen-Specific CD8+ T Cells

390 views

Measuring Microglial Uptake of Intravitreally-Delivered Fluorescent Particles Using Flow Cytometry
4:34

Measuring Microglial Uptake of Intravitreally-Delivered Fluorescent Particles Using Flow Cytometry

344 views

A Flow Cytometry Assay to Identify Immune Cell Subsets in Peripheral Blood Mononuclear Cells
4:57

A Flow Cytometry Assay to Identify Immune Cell Subsets in Peripheral Blood Mononuclear Cells

946 views

Real-Time Assessment of the Antifungal Activity of Primary Human Immune Cells
2:22

Real-Time Assessment of the Antifungal Activity of Primary Human Immune Cells

286 views

An Assay to Quantify Cytosolic and Vacuolar <em>Salmonella</em> in Infected Primary Macrophages
6:27

An Assay to Quantify Cytosolic and Vacuolar Salmonella in Infected Primary Macrophages

366 views

A Cellular Assay for the Identification of CXCR4-Interacting Agents
5:52

A Cellular Assay for the Identification of CXCR4-Interacting Agents

278 views

Determining the Role of Neutrophil-Like Cells in Lymphoma Cell Sensitivity to a Test Drug
4:23

Determining the Role of Neutrophil-Like Cells in Lymphoma Cell Sensitivity to a Test Drug

303 views

An Immunological Technique to Monitor Adjuvant-Mediated Cytotoxic T Lymphocyte Generation
4:45

An Immunological Technique to Monitor Adjuvant-Mediated Cytotoxic T Lymphocyte Generation

348 views

An Assay to Evaluate Immunodominance in Cytotoxic T Lymphocyte Responses
3:00

An Assay to Evaluate Immunodominance in Cytotoxic T Lymphocyte Responses

316 views

A Technique to Assess the Inhibitory Effect of Toxin Exposure on Miniature Excitatory Postsynaptic Currents (mEPSCs)
4:37

A Technique to Assess the Inhibitory Effect of Toxin Exposure on Miniature Excitatory Postsynaptic Currents (mEPSCs)

408 views

Measuring Macrophage Phagocytic Efficiency with Primaquine and Photodynamic Therapy
3:45

Measuring Macrophage Phagocytic Efficiency with Primaquine and Photodynamic Therapy

484 views

An Assay to Study the Impact of Photodynamic Treatment with a Photosensitizer on Macrophage Metabolic Activity
3:10

An Assay to Study the Impact of Photodynamic Treatment with a Photosensitizer on Macrophage Metabolic Activity

420 views

The Effect of Aspirin and Ibuprofen on the Phagocytic Ability of Macrophages
3:06

The Effect of Aspirin and Ibuprofen on the Phagocytic Ability of Macrophages

545 views

Isolation and Digestion of Neutrophil Proteins
4:50

Isolation and Digestion of Neutrophil Proteins

644 views

A STAGE Tip Procedure for Desalting and Purification of Peptides
5:43

A STAGE Tip Procedure for Desalting and Purification of Peptides

1.4K views

Imaging and Visualization Techniques

30 Videos

132 Minutes

Calcein Acetoxymethyl Staining to Evaluate Natural Killer Cell-Mediated Cytotoxicity toward Cancer Cells
2:53

Calcein Acetoxymethyl Staining to Evaluate Natural Killer Cell-Mediated Cytotoxicity toward Cancer Cells

534 views

In Vitro Stimulation and Visualization of Macrophage Extracellular Traps
4:31

In Vitro Stimulation and Visualization of Macrophage Extracellular Traps

508 views

Reporter Enzyme Fluorescence Imaging for In Vivo Tracking of Bacterial Infection
5:26

Reporter Enzyme Fluorescence Imaging for In Vivo Tracking of Bacterial Infection

378 views

Assessment of <em>Leishmania Virulence</em> within Cultured Macrophages
4:46

Assessment of Leishmania Virulence within Cultured Macrophages

425 views

Assessment of Intracellular Growth of <em>Coxiella</em> Mutants within Eukaryotic Cells
5:13

Assessment of Intracellular Growth of Coxiella Mutants within Eukaryotic Cells

302 views

Visualization of Internalized Anaerobic Bacteria within Host Endothelial Cells
4:19

Visualization of Internalized Anaerobic Bacteria within Host Endothelial Cells

353 views

A Technique to Assess the In Vivo Localization of Tumor-Specific Antibodies
4:13

A Technique to Assess the In Vivo Localization of Tumor-Specific Antibodies

396 views

Optical Coherence Tomography Imaging for Assessing Uveitis in a Mouse Model
3:12

Optical Coherence Tomography Imaging for Assessing Uveitis in a Mouse Model

471 views

Visualization of Bacteria in Bladder Biopsy Sections via Fluorescence In Situ Hybridization
6:10

Visualization of Bacteria in Bladder Biopsy Sections via Fluorescence In Situ Hybridization

397 views

Photoconversion-Based Leukocyte Tracking in Bacterial-Infected Transgenic Zebrafish
4:02

Photoconversion-Based Leukocyte Tracking in Bacterial-Infected Transgenic Zebrafish

361 views

Three-Dimensional Quantitative Phase Imaging for Characterizing Lymphocyte Subtypes
4:37

Three-Dimensional Quantitative Phase Imaging for Characterizing Lymphocyte Subtypes

412 views

Super-Resolution Imaging of NK Cell Immunological Synapse Formation on a Supported Lipid Bilayer
7:53

Super-Resolution Imaging of NK Cell Immunological Synapse Formation on a Supported Lipid Bilayer

381 views

An In Vitro Technique to Visualize Exocytosis of Secretory Granules in Mast Cells
3:34

An In Vitro Technique to Visualize Exocytosis of Secretory Granules in Mast Cells

414 views

A zWEDGI Technique to Visualize Fungal Pathogen-Infected Zebrafish Larvae
2:58

A zWEDGI Technique to Visualize Fungal Pathogen-Infected Zebrafish Larvae

342 views

The Photoconversion Technique for Exploring Inflammatory Cell Dynamics in Insect Pupae
3:44

The Photoconversion Technique for Exploring Inflammatory Cell Dynamics in Insect Pupae

341 views

Assessing Immunological Synapse Topology through Live-Cell Imaging
5:03

Assessing Immunological Synapse Topology through Live-Cell Imaging

351 views

An In Vivo Immunofluorescence Localization Method to Study Antibody Biodistribution
6:15

An In Vivo Immunofluorescence Localization Method to Study Antibody Biodistribution

424 views

A Luciferase-Fluorescent Reporter Influenza Virus to Track Viral Infections
3:49

A Luciferase-Fluorescent Reporter Influenza Virus to Track Viral Infections

419 views

Using Immuno-RNA Fluorescence In Situ Hybridization to Visualize SARS-CoV-2
2:59

Using Immuno-RNA Fluorescence In Situ Hybridization to Visualize SARS-CoV-2

465 views

Localized Surface Plasmon Resonance Imaging to Detect Protein Secretions from a Single Cell
5:00

Localized Surface Plasmon Resonance Imaging to Detect Protein Secretions from a Single Cell

434 views

Analyzing the Antigenic Relationship between Viruses using an Image-Based Microneutralization Assay
3:17

Analyzing the Antigenic Relationship between Viruses using an Image-Based Microneutralization Assay

369 views

An In Vivo Assay to Quantify Bacterial Phagocytosis in Adult <em>Drosophila</em> Flies
4:50

An In Vivo Assay to Quantify Bacterial Phagocytosis in Adult Drosophila Flies

347 views

Using DNA-Based Tension Probes to Assess Receptor Forces Applied by Immune Cells
5:58

Using DNA-Based Tension Probes to Assess Receptor Forces Applied by Immune Cells

274 views

Colorimetric Detection of a Bacterial Biomarker Using a Modified Litmus Test
3:04

Colorimetric Detection of a Bacterial Biomarker Using a Modified Litmus Test

370 views

A Luciferase Reporter Assay to Study Translation Regulation in Poxvirus-Infected Cells
4:07

A Luciferase Reporter Assay to Study Translation Regulation in Poxvirus-Infected Cells

433 views

Quantitative Time-Lapse Microscopy for Assessing Extracellular Matrix Stiffness-Dependent Bacterial Dissemination
4:24

Quantitative Time-Lapse Microscopy for Assessing Extracellular Matrix Stiffness-Dependent Bacterial Dissemination

298 views

TIRF Microscopy-Based Visualization of Phagosome Formation and Closure
4:13

TIRF Microscopy-Based Visualization of Phagosome Formation and Closure

389 views

Real-Time Analysis of Resident T-cell Migration in Different Tumor Regions Using Confocal Microscopy
4:23

Real-Time Analysis of Resident T-cell Migration in Different Tumor Regions Using Confocal Microscopy

326 views

An Immunofluorescence Method to Measure the Neutralization of Human Cytomegalovirus
4:05

An Immunofluorescence Method to Measure the Neutralization of Human Cytomegalovirus

554 views

Using <sup>23</sup>Na Magnetic Resonance Imaging to Measure Sodium in Muscle Tissues
3:16

Using 23Na Magnetic Resonance Imaging to Measure Sodium in Muscle Tissues

959 views

In Vitro and In Vivo Models

19 Videos

77 Minutes

Establishing an Infection Model Through Microinjection of Gastric Organoids with Pathogenic Bacteria
3:30

Establishing an Infection Model Through Microinjection of Gastric Organoids with Pathogenic Bacteria

492 views

<em>Candida albicans</em> Biofilm Formation in an In Vivo Mouse Model
3:50

Candida albicans Biofilm Formation in an In Vivo Mouse Model

536 views

Induction of Necrotizing Enterocolitis in Human Epithelial Enteroid Model
5:31

Induction of Necrotizing Enterocolitis in Human Epithelial Enteroid Model

250 views

A Mouse Tail Snip Procedure for Peripheral Blood Collection and Plasma Isolation
2:51

A Mouse Tail Snip Procedure for Peripheral Blood Collection and Plasma Isolation

2.6K views

An In Vitro Technique for UV Irradiation-Mediated Generation of Apoptotic T Cells
3:54

An In Vitro Technique for UV Irradiation-Mediated Generation of Apoptotic T Cells

1.1K views

Agarose-Based Preparation of Human Precision-Cut Lung Slices
4:26

Agarose-Based Preparation of Human Precision-Cut Lung Slices

932 views

An Assay to Measure Chemically-Induced Cytotoxicity in Human Precision-Cut Lung Slices
3:10

An Assay to Measure Chemically-Induced Cytotoxicity in Human Precision-Cut Lung Slices

455 views

Bacterial Culture Filtrate Protein Concentration using an Ultrafiltration Device
2:49

Bacterial Culture Filtrate Protein Concentration using an Ultrafiltration Device

538 views

A Size Exclusion Chromatography Technique to Purify Mycobacterial Extracellular Vesicles
4:38

A Size Exclusion Chromatography Technique to Purify Mycobacterial Extracellular Vesicles

432 views

Measuring Immune-Induced Inhibition of Seedling Growth
2:29

Measuring Immune-Induced Inhibition of Seedling Growth

361 views

An Assay to Evaluate Drug Efficacy against a <em>Mycobacterium tuberculosis</em> Infection Model
5:14

An Assay to Evaluate Drug Efficacy against a Mycobacterium tuberculosis Infection Model

381 views

A Focus-Forming Assay for Quantifying Virus Titers in the Organs of an Infected Mouse
6:23

A Focus-Forming Assay for Quantifying Virus Titers in the Organs of an Infected Mouse

640 views

Assessing the Antimicrobial Potential of Bacteriocin-Producing Murine Fecal Bacterial Isolates
2:49

Assessing the Antimicrobial Potential of Bacteriocin-Producing Murine Fecal Bacterial Isolates

368 views

A Riboflavin and UV Light Treatment of Platelet Products to Eliminate Bacteria
5:09

A Riboflavin and UV Light Treatment of Platelet Products to Eliminate Bacteria

287 views

In Vitro Measurement of α-Galactosidase A and Acid α-Glucosidase Enzyme Activity
3:20

In Vitro Measurement of α-Galactosidase A and Acid α-Glucosidase Enzyme Activity

436 views

An In Vitro Broth Microdilution Assay for the Screening of Antifungal Compounds
3:48

An In Vitro Broth Microdilution Assay for the Screening of Antifungal Compounds

545 views

An In Vitro Assay for Evaluating the Immunomodulatory Impact of Monocytes on Leukocyte Proliferation
2:46

An In Vitro Assay for Evaluating the Immunomodulatory Impact of Monocytes on Leukocyte Proliferation

295 views

An In Vitro Flow Investigation of the Effect of Stromal Cells on Leukocyte Recruitment
5:52

An In Vitro Flow Investigation of the Effect of Stromal Cells on Leukocyte Recruitment

336 views

A Method for Sample Preparation to Visualize Biofilm Forming Bacteria on Fungal Hyphae
4:37

A Method for Sample Preparation to Visualize Biofilm Forming Bacteria on Fungal Hyphae

420 views

Specialized Assays

67 Videos

280 Minutes

Real-Time Imaging of Macrophage Phagocytosis of IgG-Opsonized Red Blood Cells
4:33

Real-Time Imaging of Macrophage Phagocytosis of IgG-Opsonized Red Blood Cells

885 views

Measurement of Natural Killer Cell Migration in Response to Chemotactic Stimuli
2:29

Measurement of Natural Killer Cell Migration in Response to Chemotactic Stimuli

515 views

A Serum Bactericidal Assay for the Complement-Mediated Bactericidal Activity of Antibodies
5:40

A Serum Bactericidal Assay for the Complement-Mediated Bactericidal Activity of Antibodies

993 views

The Hemagglutination Inhibition Assay to Detect Serum Antibodies Against a Target Antigen
3:59

The Hemagglutination Inhibition Assay to Detect Serum Antibodies Against a Target Antigen

1.9K views

An Assay for Bacterial Extracellular Protein-Mediated Cytotoxicity on PMNs
3:14

An Assay for Bacterial Extracellular Protein-Mediated Cytotoxicity on PMNs

270 views

Cytotoxicity Assay of <em>Staphylococcus aureus</em> Strains on Neutrophils following Phagocytosis
3:35

Cytotoxicity Assay of Staphylococcus aureus Strains on Neutrophils following Phagocytosis

393 views

A Bacterial Infection Assay for Studying Anaerobic Bacteria-Host Cell Interactions
5:28

A Bacterial Infection Assay for Studying Anaerobic Bacteria-Host Cell Interactions

778 views

Assessment of Opsonophagocytic Killing Activity against Bacterial Pathogens
4:29

Assessment of Opsonophagocytic Killing Activity against Bacterial Pathogens

532 views

Assessing Internalized <em>Porphyromonas gingivalis</em> in Host Endothelial Cells
2:37

Assessing Internalized Porphyromonas gingivalis in Host Endothelial Cells

345 views

An In Vitro Assay to Study the Cytotoxic Capability of Cytokine-Induced Killer Cells
5:27

An In Vitro Assay to Study the Cytotoxic Capability of Cytokine-Induced Killer Cells

422 views

In Vitro Adhesion Assay to Detect the Adherence of Cancer Cells to Neutrophil Extracellular Traps
4:25

In Vitro Adhesion Assay to Detect the Adherence of Cancer Cells to Neutrophil Extracellular Traps

486 views

Evaluating Cytotoxicity and Membrane Integrity in Lung Slices via Lactate Dehydrogenase Assay
2:58

Evaluating Cytotoxicity and Membrane Integrity in Lung Slices via Lactate Dehydrogenase Assay

403 views

A Fluorescence-Based Assay Using Engineered Lymphocytes for Screening Immunomodulatory Compounds
2:57

A Fluorescence-Based Assay Using Engineered Lymphocytes for Screening Immunomodulatory Compounds

341 views

Monitoring B Cell Activation with Antigenic Liposomes using a Calcium-Flux Assay
3:54

Monitoring B Cell Activation with Antigenic Liposomes using a Calcium-Flux Assay

438 views

A Technique to Quantify Fungal Infection in Zebrafish Larvae
2:24

A Technique to Quantify Fungal Infection in Zebrafish Larvae

406 views

An Opsono-Adherence Assay for Assessing the Opsonization of Encapsulated Bacteria by Anti-Capsule Antibodies
6:34

An Opsono-Adherence Assay for Assessing the Opsonization of Encapsulated Bacteria by Anti-Capsule Antibodies

312 views

A Bead-Based Multiplex Immunoassay to Identify Pathogen-Specific Antibodies in Saliva
3:45

A Bead-Based Multiplex Immunoassay to Identify Pathogen-Specific Antibodies in Saliva

338 views

Quantitative Flow Cytometric Measurement of Fungal Spore Phagocytosis by Human Phagocytes
5:28

Quantitative Flow Cytometric Measurement of Fungal Spore Phagocytosis by Human Phagocytes

343 views

An Immunofluorescence Assay to Characterize and Quantify Mammalian Stress Granules
4:34

An Immunofluorescence Assay to Characterize and Quantify Mammalian Stress Granules

382 views

An Assay to Assess Phagocytosis and Oxidative Burst Activity In Granulocytes and Monocytes
4:59

An Assay to Assess Phagocytosis and Oxidative Burst Activity In Granulocytes and Monocytes

349 views

Quantification of Immune Mediator Levels in Human Mucosal Lining Fluid
2:55

Quantification of Immune Mediator Levels in Human Mucosal Lining Fluid

247 views

A Fluorogenic Peptide Cleavage Assay to Screen the Proteolytic Activity of Proteases
2:42

A Fluorogenic Peptide Cleavage Assay to Screen the Proteolytic Activity of Proteases

370 views

An Assay for Quantifying Antibody-Mediated Phagocytosis of Parasite-Infected Erythrocytes
6:56

An Assay for Quantifying Antibody-Mediated Phagocytosis of Parasite-Infected Erythrocytes

352 views

An In Vitro Assay for Measuring Neutrophil Serine Protease Activity Using a Fluorescent Reporter
2:59

An In Vitro Assay for Measuring Neutrophil Serine Protease Activity Using a Fluorescent Reporter

339 views

An In Vitro Assay for Studying the Cytotoxicity of Pre-Activated CD8<sup>+</sup> T Cells against Cancer Cells
5:04

An In Vitro Assay for Studying the Cytotoxicity of Pre-Activated CD8+ T Cells against Cancer Cells

575 views

A Microneutralization Assay to Measure Neutralizing Antibody Titers in Human Serum
5:33

A Microneutralization Assay to Measure Neutralizing Antibody Titers in Human Serum

575 views

An Assay to Detect Oxidative Burst in <em>Arabidopsis</em> Leaf Discs upon Immune Elicitation
2:58

An Assay to Detect Oxidative Burst in Arabidopsis Leaf Discs upon Immune Elicitation

680 views

An Assay for Detecting Reactive Oxygen Species Production in Rice Tissues upon Immune Elicitation
3:42

An Assay for Detecting Reactive Oxygen Species Production in Rice Tissues upon Immune Elicitation

436 views

An Assay for Studying Fcγ Receptor-Driven Phagocytosis in Monocyte Monolayers
3:30

An Assay for Studying Fcγ Receptor-Driven Phagocytosis in Monocyte Monolayers

300 views

A Charcoal Agar Resazurin Assay to Evaluate Test Compound Activity Against Mycobacteria
6:08

A Charcoal Agar Resazurin Assay to Evaluate Test Compound Activity Against Mycobacteria

350 views

An Assay for TLR-Dependent NF-&kcy;B/AP-1 Transcription Factor Signaling in Macrophages
4:08

An Assay for TLR-Dependent NF-кB/AP-1 Transcription Factor Signaling in Macrophages

283 views

A Real-Time In Vitro Cytolysis Assay to Evaluate the Potency of CAR T Cells
3:11

A Real-Time In Vitro Cytolysis Assay to Evaluate the Potency of CAR T Cells

473 views

An In Vitro Method to Determine the Anti-Apoptotic Activity of Antibodies against TNFα
5:54

An In Vitro Method to Determine the Anti-Apoptotic Activity of Antibodies against TNFα

293 views

A Vaccine-Pulsed Dendritic Cell-Based In Vitro Assay to Induce Lymphocyte Proliferation
5:16

A Vaccine-Pulsed Dendritic Cell-Based In Vitro Assay to Induce Lymphocyte Proliferation

401 views

A Hemocyte Disturbance Assay to Assess Hemocyte Re-Adhesion to Hematopoietic Pockets
2:19

A Hemocyte Disturbance Assay to Assess Hemocyte Re-Adhesion to Hematopoietic Pockets

305 views

An In Vitro Assay to Examine the Antibody-Dependent Enhancement of Viral Infection
5:35

An In Vitro Assay to Examine the Antibody-Dependent Enhancement of Viral Infection

339 views

An In Vitro Assay to Quantify the Intracellular Growth of Parasite in Macrophages
3:29

An In Vitro Assay to Quantify the Intracellular Growth of Parasite in Macrophages

335 views

A Dye Release Assay to Quantify Enzymatic Activity of Antimicrobial Proteins
6:01

A Dye Release Assay to Quantify Enzymatic Activity of Antimicrobial Proteins

474 views

A Spot Plate Assay for Evaluating Oxidative Stress Responses in Bacterial Strains
5:10

A Spot Plate Assay for Evaluating Oxidative Stress Responses in Bacterial Strains

740 views

Evaluating the Effectiveness of a Test Compound against Mycobacteria in Broth
2:20

Evaluating the Effectiveness of a Test Compound against Mycobacteria in Broth

312 views

A Peg Plate Biofilm Assay for Screening Antibacterial Agents
6:41

A Peg Plate Biofilm Assay for Screening Antibacterial Agents

595 views

A Barcoding Assay for Phenotypic and Functional Characterization of Immune Cells
5:42

A Barcoding Assay for Phenotypic and Functional Characterization of Immune Cells

306 views

A CFSE-Based Assay to Monitor Skin Dendritic Cell Migration to Draining Lymph Nodes in Infected Mice
4:34

A CFSE-Based Assay to Monitor Skin Dendritic Cell Migration to Draining Lymph Nodes in Infected Mice

365 views

Utilizing Bead-Supported Lipid Bilayers to Investigate the Synaptic Output from T Cells
3:49

Utilizing Bead-Supported Lipid Bilayers to Investigate the Synaptic Output from T Cells

294 views

A Deferred Growth Inhibition Assay to Evaluate the Effect of Bacteria-Derived Antimicrobial Compounds
3:37

A Deferred Growth Inhibition Assay to Evaluate the Effect of Bacteria-Derived Antimicrobial Compounds

471 views

A Turbidimetric Analysis to Monitor Collagen-Induced Aggregation of Pretreated Human Platelets
5:42

A Turbidimetric Analysis to Monitor Collagen-Induced Aggregation of Pretreated Human Platelets

392 views

Assessing the Effectiveness of an Antiviral Test Compound through a Viral Inactivation Assay
3:41

Assessing the Effectiveness of an Antiviral Test Compound through a Viral Inactivation Assay

338 views

A Viral Attachment Assay for Screening of Antiviral Compounds
2:47

A Viral Attachment Assay for Screening of Antiviral Compounds

752 views

An Assay to Evaluate the Effect of Test Compounds on Viral Entry and Fusion in Host Cells
2:38

An Assay to Evaluate the Effect of Test Compounds on Viral Entry and Fusion in Host Cells

371 views

A Molecular-Based Assay for Quantifying Influenza A and B Viruses
3:49

A Molecular-Based Assay for Quantifying Influenza A and B Viruses

337 views

A Microtiter Plate Method to Assess the Minimum Inhibitory Concentration of an Antibiotic
5:16

A Microtiter Plate Method to Assess the Minimum Inhibitory Concentration of an Antibiotic

1.2K views

An Assay to Study the Impact of Extracellular Matrix Stiffness on Bacterial Infection
4:10

An Assay to Study the Impact of Extracellular Matrix Stiffness on Bacterial Infection

341 views

Determining Antiviral Efficacy of Experimental Drugs Through a Plaque Assay
2:48

Determining Antiviral Efficacy of Experimental Drugs Through a Plaque Assay

774 views

An Assay to Determine Polymicrobial Biofilm Initiation on Virus-Infected Cells
3:57

An Assay to Determine Polymicrobial Biofilm Initiation on Virus-Infected Cells

327 views

An ATP Utilization Assay for Determining Bacterial Survival Under Antibiotics Treatment
2:56

An ATP Utilization Assay for Determining Bacterial Survival Under Antibiotics Treatment

351 views

Flow Cytometry-Based Quantification of Therapy-Induced Senescent Cancer Cells
4:19

Flow Cytometry-Based Quantification of Therapy-Induced Senescent Cancer Cells

462 views

Detecting Bacterial Contamination Using Magneto-fluorescent Nanosensors
3:08

Detecting Bacterial Contamination Using Magneto-fluorescent Nanosensors

305 views

A Solid Phase Adherence Assay to Assess Biofilm Formation
4:07

A Solid Phase Adherence Assay to Assess Biofilm Formation

545 views

An Assay to Study Bile-Salt Induced Biofilm Formation through EPS Matrix Detection
2:50

An Assay to Study Bile-Salt Induced Biofilm Formation through EPS Matrix Detection

354 views

Detection of Biofilm Disassembly Through a Dispersion Assay
2:49

Detection of Biofilm Disassembly Through a Dispersion Assay

484 views

An Assay for Real-Time Monitoring of Extracellular pH in Dental Biofilms
6:52

An Assay for Real-Time Monitoring of Extracellular pH in Dental Biofilms

282 views

A FRET Flow Cytometry Technique to Detect Tau-Seed Induced Reporter Protein Aggregation
4:11

A FRET Flow Cytometry Technique to Detect Tau-Seed Induced Reporter Protein Aggregation

454 views

Multiplexed Quantitative Bacterial Detection using Dye-Labeled Magnetic Microspheres
3:54

Multiplexed Quantitative Bacterial Detection using Dye-Labeled Magnetic Microspheres

469 views

Assessing Functional SARS-CoV-2 Neutralizing Antibodies
5:30

Assessing Functional SARS-CoV-2 Neutralizing Antibodies

979 views

A Degranulation Assay to Monitor Neutrophil Activation and Gelatinase Release
3:50

A Degranulation Assay to Monitor Neutrophil Activation and Gelatinase Release

915 views

An Assay to Monitor Degranulation of Azurophilic Granules in Neutrophils following Activation
3:05

An Assay to Monitor Degranulation of Azurophilic Granules in Neutrophils following Activation

542 views

Assays for Qualitative and Quantitative Assessment of Mouse Urine Glucose Concentrations
4:42

Assays for Qualitative and Quantitative Assessment of Mouse Urine Glucose Concentrations

878 views

Why Choose JoVE Encyclopedia of Experiments

Boost Research Productivity

Save time mastering new protocols and efficiently onboarding and training lab members

Optimize Lab Resources

Reduce lab expenses and conserve valuable resources

Advance Your Career:

Build essential skills and expand your expertise quickly learning new research technologies.