Name: fermentation pathways without oxygen
Uploaded: 2019-03-11T09:21:11.000Z
Duration: 1 min 29 s
Channel: JoVE

K12 Standards

Tennessee Academic Standards

High School

Biology

from-molecules-to-organisms-structures-and-processes

From Molecules to Organisms: Structures and Processes

BIO1.LS1.9

Fermentation

תסיסה היא תהליך אנאירובי של שימוש בסוכר ללא חמצן. לדוגמה, במהלך תסיסת חומצה לקטית, כאשר נגמר לשרירים החמצן, גלוקוז מתפרק לשתי מולקולות פירובט במהלך הגליקוליזה, מייצר שני ATP, ומחזר שתי מולקולות NAD ל-NADH. אז, ה-NADH מגיב עם פירובט כדי לייצר לקטט, ללא שחרור פחמן דו-חמצני וללא ייצור נוסף של ATP.למעשה, כדי לייצר את אותה הכמות של ATP כמו בנשימה אירובית, התא חייב לצרוך כמויות גדולות בהרבה של סוכר. לאחר חמצון NADH, מוזן בחזרה לגליקוליזה NAD+שבה ה-ATP נאסף על-ידי התפרקות הגלוקוז. בבני אדם, כאשר החמצן הוא מועט, תאי השריר יכולים לייצר ATP בתהליך זה במהלך פעילות גופנית מאומצת.עודף הלקטט שהצטבר מועבר אז אל הכבד באמצעות הדם, שם הוא מומר בחזרה לפירובט.

תסיסה

<div style="direction:rtl">רוב האורגניזמים האוקריוטיים דורשים חמצן כדי לשרוד ולתפקד כראוי. אורגניזמים כאלה מייצרים כמויות גדולות של אנרגיה במהלך הנשימה האירובית על ידי חילוף חומרים של גלוקוז וחמצן לפחמן דו חמצני ומים. עם זאת, רוב האיקריוטים יכולים לייצר אנרגיה מסוימת בהיעדר חמצן על ידי חילוף חומרים אנאירובי. תסיסה היא סוג של תהליך מטבולי המתרחש בהיעדר חמצן, שבו מולקולות אורגניות כגון גלוקוז מתפרקות להפקת אנרגיה. במהלך תהליך זה, מולקולת הגלוקוז מומרת תחילה לשתי מולקולות של פירובט באמצעות סדרה של תגובות כימיות. תהליך זה נקרא גליקוליזה והוא זהה לשלב הראשון בנשימה אירובית. בהיעדר חמצן, מולקולות הפירובט מומרות למולקולות אורגניות שונות באמצעות מגוון מסלולים בהתאם לאורגניזם המעורב. היבט מרכזי אחד של תסיסה הוא התחדשות של +^NAD מ-NADH. במהלך הגליקוליזה, +^NAD הופך ל-NADH, והמשך תפקוד הגליקוליזה מצריך התחדשות של +^NAD. בהיעדר חמצן, תסיסה היא התהליך שמחדש את +^NAD על ידי העברת אלקטרונים מ-NADH למולקולה אורגנית, כגון פירובט, וכתוצאה מכך נוצרת מולקולה אורגנית חדשה +^NAD. ישנם שני סוגי תסיסה עיקריים המבוססים על התוצרים הסופיים של התגובה: תסיסת חומצת חלב ותסיסת אלכוהול. אצל יונקים, תסיסה של חומצת חלב מתרחשת בתאי דם אדומים שאינם יכולים לנשום בצורה אירובית בגלל היעדר מיטוכונדריה, וכן בשרירי השלד בזמן פעילות גופנית מאומצת. זה מופיע גם בחיידקים מסוימים, כמו אלה שנמצאים ביוגורט. בתגובה זו, פירובט ו-NADH הופכים לחומצה לקטית ול-+^NAD. תסיסת אלכוהול היא תהליך דו-שלבי. בשלב הראשון, פירובט הופך לפחמן דו חמצני ואצטאלדהיד. בשלב השני, אצטלדהיד פועל כמקבל אלקטרונים ומופחת לאתנול עם המרה נלווית של NADH +^NAD. בסך הכל, תסיסת אלכוהול הופכת פירובט ו-NADH לאתנול, פחמן דו חמצני +^NAD. שמרים משתמשים בתסיסת אלכוהול כדי להמיר סוכרים לפחמן דו חמצני ואתנול. תהליך זה נרתם לייצור משקאות אלכוהוליים כגון בירה ויין.</div>

רוב האורגניזמים האיקריוטים זקוקים לחמצן כדי לשרוד ולתפקד כראוי. אורגניזמים כאלה מייצרים כמויות גדולות של אנרגיה במהלך נשימה אירובית על ידי ...

Education

JoVE Core

ביולוגיה

Cellular Processes

תהליכים תאיים

Cellular Respiration

נשימה תאית

KEY TERMS AND CONCEPTS

מונחים ומושגים מרכזיים

fermentation pathways without oxygen

Fermentation is a metabolic pathway that lets cells make energy without oxygen. It begins after glucose is split into two pyruvate molecules during glycolysis. Glycolysis is the same first step used in aerobic respiration.

When oxygen is unavailable, pyruvate does not enter aerobic respiration. Instead, cells convert pyruvate into other organic molecules through pathways that depend on the organism. A key purpose of fermentation is to regenerate NAD+ from NADH so glycolysis can keep running.

During glycolysis, NAD+ becomes NADH. Fermentation transfers electrons from NADH to an organic molecule, such as pyruvate. This reaction produces a new organic molecule and restores NAD+.

The two main fermentation types are lactic acid fermentation and alcohol fermentation. Lactic acid fermentation occurs in red blood cells, which lack mitochondria, and in skeletal muscles during strenuous exercise. It also happens in certain bacteria, including those found in yogurt. In this pathway, pyruvate and NADH are converted to lactic acid and NAD+.

Alcohol fermentation happens in two steps. First, pyruvate is changed into carbon dioxide and acetaldehyde. Then acetaldehyde accepts electrons and is reduced to ethanol, while NADH becomes NAD+. Overall, alcohol fermentation turns pyruvate and NADH into ethanol, carbon dioxide, and NAD+.

Yeasts use alcohol fermentation to convert sugars into carbon dioxide and ethanol. People harness this process to produce alcoholic beverages such as beer and wine. Fermentation shows how cells can keep making energy when oxygen is limited.

Fermentation Pathways Without Oxygen

what is glycolysis?

Glycolysis: How Cells Break Down Glucose

Glycolysis is the first stage of cellular respiration and a key way cells break down glucose to make energy. Cellular respiration converts food energy from macromolecules into ATP, the cell’s main energy currency. Glycolysis also creates intermediate molecules that can feed into other pathways. In this pathway, one six-carbon sugar such as glucose is split into two three-carbon molecules, including pyruvate. The process produces a net gain of two ATP molecules, since four ATP are made and two...

energy-requiring steps of glycolysis

Glycolysis: Glucose Trapping and Splitting

Glycolysis begins in the cytosol, where glucose is prepared for use as energy. This process has two phases: an energy-requiring phase and an energy-releasing phase. The first phase changes glucose into a more reactive, unstable form before it can move into later steps. In the first step, glucose is phosphorylated, which means a phosphate group is added from ATP. This produces glucose 6-phosphate. The added phosphate is negatively charged, so it cannot cross the hydrophobic cell membrane. As a...

energy-releasing steps of glycolysis

Glycolysis: ATP and NADH Formation

Glycolysis produces ATP and NADH during its energy-releasing phase. These reactions are the last steps of glycolysis and convert glyceraldehyde-3-phosphate, or G3P, into pyruvate. The phase is often described as steps 6 to 10, and it happens twice because two 3-carbon sugars are made earlier in glycolysis. The sixth step begins with two linked events. G3P is oxidized, which means it loses hydrogen, and it is phosphorylated, which means a phosphate group is added. NAD+ acts as the electron...

pyruvate oxidation

How Pyruvate Becomes Acetyl CoA

Pyruvate is prepared for the next stage of cellular respiration inside the mitochondria. After glycolysis, the charged pyruvate molecules enter the mitochondria by active transport. There, they go through three enzymatic reactions so the energy stored in pyruvate can be used by the cell. First, pyruvate dehydrogenase removes the carboxyl group from pyruvate. This step releases carbon dioxide. Next, the stripped molecule is oxidized, and the electrons are picked up by NAD+ to make NADH. The...

the citric acid cycle

Citric Acid Cycle Energy and Products

The citric acid cycle is a key energy-making pathway in cells. It is also called the Krebs cycle or the TCA cycle. In eukaryotic cells, it takes place in the matrix of mitochondria. In prokaryotic cells, it occurs in the cytoplasm. The cycle starts with acetyl CoA, which enters the pathway after pyruvate oxidation. That earlier step makes two acetyl CoA molecules per glucose molecule. For that reason, the citric acid cycle runs twice for each glucose molecule. The pathway includes eight...

electron transport chains

ATP Production by the Electron Transport Chain

ATP production in the electron transport chain is the final stage of cellular respiration. This stage is part of oxidative phosphorylation, which has two steps: the electron transport chain and chemiosmosis. In eukaryotic cells, the electron transport chain sits in the inner mitochondrial membrane. Its main job is to build a proton gradient. That gradient is then used during chemiosmosis to make ATP. The process also regenerates the electron carriers NAD+ and FAD, which are needed again in...

chemiosmosis

ATP Production by Chemiosmosis

ATP production by chemiosmosis is part of oxidative phosphorylation, the efficient stage of cellular respiration that makes most of the cell’s ATP. Oxidative phosphorylation includes two linked processes: the electron transport chain and chemiosmosis. Together, they help generate adenosine triphosphate, or ATP, the main energy molecule used by cells. The electron transport chain is a series of protein complexes in the inner mitochondrial membrane. These complexes pass electrons through a set...

electron carriers

Electron Carriers in Cellular Respiration

Electron carriers help move electrons through cellular respiration. They act like shuttles that can accept electrons, which means they are reduced, or give electrons away, which means they are oxidized. This changing between oxidized and reduced forms is essential for energy production. As glucose breaks down into carbon dioxide and water, it loses electrons at several stages. Electron carriers pick up those electrons and store them temporarily. They then release the electrons into the...

fermentation

Fermentation is a metabolic pathway that lets cells make energy without oxygen. It begins after glucose is split into two pyruvate molecules during glycolysis. Glycolysis is the same first step used in aerobic respiration. When oxygen is unavailable, pyruvate does not enter aerobic respiration. Instead, cells convert pyruvate into other organic molecules through pathways that depend on the organism. A key purpose of fermentation is to regenerate NAD+ from NADH so glycolysis can keep running. <br...

dietary connections

How Food Becomes ATP

Food becomes ATP through connected steps in cellular respiration. Carbohydrates, proteins, and lipids all feed into these pathways. The body can use foods such as apples, cheese, and guacamole as sources of energy. Carbohydrate digestion starts with enzymes that break complex carbohydrates into simple sugars. Glucose can enter glycolysis directly. Fructose and galactose must first be converted into sugars that are intermediates of the glycolytic pathway. Proteins are digested into amino acids.

introduction to cellular respiration

Cellular Respiration: ATP Production Steps

Cellular respiration is the process cells use to turn energy from food into ATP. ATP, or adenosine triphosphate, stores energy in chemical bonds that can be released quickly when cells need it. Much of the energy released during respiration becomes heat, but some of it is captured to make ATP. This process depends on oxidation-reduction, or redox, reactions. In these reactions, oxidation means electron loss and reduction means electron gain. The electron carriers NAD+ and FAD, along with their...

products of the citric acid cycle

Citric Acid Cycle Output in Cellular Respiration

The citric acid cycle is a key stage of aerobic respiration in cells. It is also called the Krebs cycle or the tricarboxylic acid (TCA) cycle. Plants and animals use this oxygen-requiring pathway as part of cellular respiration. This cycle happens after glycolysis and pyruvate oxidation. Glycolysis makes two pyruvate molecules from each glucose molecule. During pyruvate oxidation, each pyruvate becomes acetyl-CoA, which is the input for the citric acid cycle. That means one glucose molecule...

outcomes of glycolysis

Glycolysis Products in Cellular Respiration

Glycolysis is the first step of cellular respiration and breaks glucose into smaller molecules. Cells use this process to pull energy from glucose over a series of chemical reactions. Cellular respiration can happen with oxygen or without oxygen. In both aerobic and anaerobic cellular respiration, glycolysis makes a net gain of two pyruvate molecules, two NADH molecules, and two ATP molecules. Four ATP molecules are produced, but two ATP molecules are used earlier in the pathway. Glycolysis...

atp yield

How Cellular Respiration Makes ATP

Cellular respiration makes ATP from glucose in several stages. The process usually produces 30 to 32 ATP per glucose molecule. Most of that ATP comes from oxidative phosphorylation and the electron transport chain, while 4 ATP are made earlier, with 2 from glycolysis and 2 from the citric acid cycle. The electron transport chain, or ETC, sits in the inner mitochondrial membrane. It has four main protein complexes and ATP synthase, the enzyme that makes ATP. NADH and FADH2 deliver electrons to...

key terms and concepts

Explore how fermentation helps cells make energy without oxygen, regenerate NAD+, and produce lactic acid or ethanol in high school biology.

Fermentation Pathways Without Oxygen | JoVE K12

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biology

Virginia  Standards of Learning

HIgh School

Life Science

LS.4

LS.4.b

Next Generation Science Standards

HS-LS1-7

Florida Department of Education

SC.912.L.18

SC.912.L.18.6

Texas Essential Knowledge and Skills

BIO.11

BIO.11.A

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New York State Science Learning Standards

matter-and-energy-in-organisms-and-ecosystems

Matter and Energy in Organisms and Ecosystems

HS-LS1-7.

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the-tree-of-life-bacteria-archaea-and-eukaryotes

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Compare and contrast existing models, identify patterns, and use structural and functional evidence to analyze the characteristics of life. Engage in argument about the designation of viruses as non-living based on these characteristics.
EXPLANATION: Students begin to develop patterns of living and non-living organisms in kindergarten and build on detailed characteristics of different classifications of living organisms throughout elementary and middle school. Biology 1 discussions introduce viral particles and viral cycles, building on student understanding of living organisms to engage in an argument regarding the classification of a viral particle as either living or non-living.

BIO1.LS1.1

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Evaluate comparative models of various cell types with a focus on organic molecules that make up cellular structures.
EXPLANATION: Students explicitly discuss the structure and function of major cellular organelles in seventh grade. Building on this understanding, Biology I students should shift their focus to the different types of cells found in organisms and how the role of each cell type relates to its composition and the prevalence of different organelles within that cell. An example might include the absence (or enucleation) of the nucleus in red blood cells in mammals providing for increased levels of oxygen transport in organisms or the lack of centrioles in most neurons. Varying cell types can include both prokaryotic and eukaryotic cell types.

BIO1.LS1.2

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The DNA Helix

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DNA Base Pairing

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The Central Dogma

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DNA Packaging

Overview

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Organization of Genes

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Mutations

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Transcription

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Lesson: Translation
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RNA Structure

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pre-mRNA Processing

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Types of RNA

Overview
Three main types of RNA are involved in protein synthesis: messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). These RNAs ...

MicroRNAs

siRNA - Small Interfering RNAs

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piRNA - Piwi-interacting RNAs

PIWI-interacting RNAs, or piRNAs, are the most abundant short non-coding RNAs. More than 20,000 genes have been found in humans that code for piRNAs while ...

CRISPR and crRNAs

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lncRNA - Long Non-coding RNAs

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Conservation of Protein Domains Over Different Proteins

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Globular and Fibrous Proteins

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Globular ...

Intrinsically Disordered Proteins

Intrinsically disordered proteins are a group of proteins that do not fold into specific three-dimensional structures. Their structural flexibility allows ...

Protein Complex Assembly

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Conjugated Proteins

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Allosteric Proteins-ATCase

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Amyloid Fibrils

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Cooperative Allosteric Transitions

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Antibody Structure

Phosphorylation

Ligand Binding Sites

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Protein Kinases and Phosphatases

Proteins undergo chemical modifications that trigger changes in the charge, structure, and conformation of the proteins. Phosphorylation, acetylation, ...

Protein-protein Interfaces

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GTPases and their Regulation

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Covalently Linked Protein Regulators

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The Equilibrium Binding Constant and Binding Strength

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protein-complexes-with-interchangeable-parts

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BIO1.LS1.3

Fermentation Pathways Without Oxygen

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