Crosslinking Solution
Crosslinking Solution - Related Videos
03:47
Crosslinking Neuronal Surface Proteins in the Mouse Brain using a Chemical Crosslinking Assay
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2025
This video demonstrates the crosslinking of neuronal surface proteins using Bis-sulfosuccinimidyl suberate or BS3. Isolated and chilled mouse brain is sectioned coronally. The desired region is extracted from the sections, minced, and treated with BS3. BS3 crosslinks surface proteins like GABA receptors, leaving internal proteins unaltered. The reaction is quenched with glycine before processing the samples for further analysis.
11:15
Nanosponge Tunability in Size and Crosslinking Density
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Cited by 1 •
2017
This article describes a process for tuning the size and crosslinking density of covalently crosslinked nanoparticles from linear polyesters containing pendant functionality. By tailoring synthesis parameters (polymer molecular weight, pendant functionality incorporation, and crosslinker equivalents), a desired nanoparticle size and crosslinking density can be achieved for drug delivery applications.
09:06
Preparation of DNA-crosslinked Polyacrylamide Hydrogels
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Cited by 5 •
2014
Our laboratory has developed DNA-crosslinked polyacrylamide hydrogels, a dynamic hydrogel system, to better understand the effects of modulating tissue stiffness on cell function. Here, we provide schematics, descriptions, and protocols to prepare these hydrogels.
07:27
Making Solutions in the Laboratory
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2023
The ability to successfully make solutions is a basic laboratory skill performed in virtually all biological and chemical experiments. A solution is a homogenous mixture of solute dissolved in bulk liquid known as the solvent. Solutions can be described by their solute concentration, a measure of how much solute is present per unit of solution. In this video, a step-by-step procedure for how to make a water-based, or aqueous, solution for biological applications is presented. The video...
09:49
Solutions and Concentrations
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2023
Source: Laboratory of Dr. Michael Evans — Georgia Institute of Technology A solution is a homogeneous mixture containing some components in small amounts, called solutes, and one component in a large amount, called the solvent. Solid-liquid solutions contain one or more solid solutes dissolved in a liquid solvent. Solutions are ubiquitous in chemistry: they are used to store and handle small amounts of material, carry out chemical reactions, and develop materials with controllable properties.
FAQs
Covalent crosslinkers create chemical bonds between reactive functional groups, whereas ionic formulations rely on charge-based interactions and physical systems form networks through noncovalent interactions. These mechanisms give researchers different ways to adjust material behavior. The choice affects the resulting network’s mechanical strength, chemical stability, elasticity, and biological suitability in applications such as hydrogels and tissue scaffolds.
pH, temperature, concentration, and light exposure can control how a crosslinking formulation forms its network. Adjusting these conditions changes the extent and character of interactions among polymer chains or biomolecules. Researchers use this control to tune properties such as stiffness, porosity, swelling, degradation resistance, and compatibility for a particular bioengineering use.
The chemistry determines how the connected network responds mechanically, chemically, and biologically. A formulation may be selected to increase strength and resistance to degradation, or to produce greater elasticity, swelling, or compatibility. Because these properties are linked to the network formed under the chosen conditions, crosslinker selection is central to matching a material with its intended biomedical function.
A typical workflow begins by identifying the required material properties, then selecting a compatible crosslinking chemistry and formulation. Researchers apply the solution to polymer chains or biomolecules and control relevant conditions, including pH, temperature, concentration, or light exposure. They then assess the resulting network for properties such as stiffness, porosity, swelling, stability, and degradation resistance.
Crosslinking formulations can support the design of hydrogels, tissue scaffolds, drug-delivery systems, and biomaterial coatings. In each case, the network helps determine functional characteristics such as mechanical strength, elasticity, swelling, porosity, and resistance to degradation. Researchers can therefore adapt the formulation and reaction conditions to the performance requirements of different biomedical materials.
Researchers match the formulation and reaction conditions to the properties required by the application. A tissue scaffold may need a suitable balance of strength, elasticity, porosity, and biological compatibility, while a drug-delivery material may require controlled swelling and stability. Adjusting the crosslinking chemistry, pH, temperature, concentration, or light exposure provides a way to tune these outcomes.