Murine Fat Tissue
Murine Fat Tissue - Related Videos
07:09
Isolation of Murine Adipose Tissue-derived Microvascular Fragments as Vascularization Units for Tissue Engineering
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Cited by 58 •
2017
We present a protocol to isolate adipose tissue-derived microvascular fragments that represent promising vascularization units. They can be rapidly isolated, do not require in vitro processing and, thus, may be used for one-step prevascularization in different fields of tissue engineering.
06:30
An ex vivo Mouse Model for High-Fat Diet-Like Adipose Tissue Inflammation
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2026
Using an ex vivo explant model, small pieces of the rostral portion of visceral white adipose tissue from lean mice are cultured for 7–10 days, enabling the induction and study of adipose tissue inflammation as it occurs during high-fat diet feeding.
13:09
Segmentation and Measurement of Fat Volumes in Murine Obesity Models Using X-ray Computed Tomography
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Cited by 26 •
2012
Fat content analysis is routinely conducted in studies utilizing murine obesity models. Emerging methods in small animal CT imaging and analysis are providing for longitudinal detail rich fat content analysis. Here we detail step by step procedures for performing small animal CT imaging, analysis, and visualization.
06:41
Decellularizing Mammary Fat Pads: Deriving Extracellular Matrix Hydrogels from Mammary Fat Pads
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2023
This video describes a technique of deriving extracellular matrix hydrogels by decellularizing murine mammary fat pads following ex vivo irradiation for in vitro studies. This hydrogel helps to mimic the in vivo breast tissue environment post-radiation treatment to study tumor cell behavior.
07:51
Isolation of Leukocytes from the Murine Tissues at the Maternal-Fetal Interface
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Cited by 44 •
2015
Described herein is a protocol to isolate and analyze the infiltrating leukocytes of tissues at the maternal-fetal interface (uterus, decidua, and placenta) of mice. This protocol maintains the integrity of most cell surface markers and yields enough viable cells for downstream applications including flow cytometry analysis.
FAQs
Nutritional and thermal conditions can shift adipocyte activity between lipid storage and mitochondrial thermogenesis. White adipocytes primarily reflect storage behavior, whereas brown and inducible beige adipocytes provide models for heat-producing responses. In bioengineering studies, these differences help researchers test whether culture conditions or material environments support the intended metabolic phenotype rather than treating all adipose cells as equivalent.
Adipocytes do not act independently of their tissue environment. Extracellular matrix provides structural context, blood vessels influence the surrounding tissue organization, and immune cells contribute to inflammatory signaling and remodeling. Including or evaluating these components helps bioengineers determine whether a scaffold or graft supports an appropriate microenvironment, rather than assessing adipocyte behavior from cell responses alone.
These adipocyte types represent different functional outcomes that a construct may need to reproduce. White cells are associated with lipid storage, while brown and inducible beige cells are linked to mitochondrial thermogenesis under appropriate conditions. Separating them allows investigators to match scaffold properties and culture conditions with goals such as adipose regeneration, metabolic function, or thermogenic tissue modeling.
Researchers can use tissue-derived cells or matrix from murine fat tissue to examine how a scaffold and its culture environment influence adipogenesis and regeneration. The experimental comparison focuses on cellular behavior in relation to material properties and conditions. This approach provides a bioengineering test system for identifying designs that better support adipose tissue formation and function.
These models support studies of adipogenesis, biomaterial design, vascularization, and engineered adipose grafts. A project may use the tissue-derived cells, matrix, or both to investigate how a construct develops and interacts with its surrounding environment. Such applications make the model useful for connecting material design decisions with tissue regeneration and metabolic performance.
Engineered adipose studies can relate material properties and cellular behavior to metabolic function, therapeutic testing, and the development of translational adipose-tissue constructs. The model also helps examine tissue remodeling and the influence of the local microenvironment. These outcomes provide a bridge between evaluating a bioengineered design in culture and considering its potential use in regenerative applications.