Nanocarrier Efficacy Screening
Nanocarrier Efficacy Screening - Related Videos
11:53
Predicting Gene Silencing Through the Spatiotemporal Control of siRNA Release from Photo-responsive Polymeric Nanocarriers
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Cited by 6 •
2017
We present a novel method that uses photo-responsive block copolymers for more efficient spatiotemporal control of gene silencing with no detectable off-target effects. Additionally, changes in gene expression can be predicted using straightforward siRNA release assays and simple kinetic modeling.
09:57
System for Efficacy and Cytotoxicity Screening of Inhibitors Targeting Intracellular Mycobacterium tuberculosis
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Cited by 15 •
2017
We have developed a modular high-throughput screening system for discovering novel compounds against Mycobacterium tuberculosis, targeting intracellular and in-broth growing bacteria as well as cytotoxicity against the mammalian host cell.
01:29
Self-Efficacy
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2025
Self-efficacy is the belief in one's capacity to organize and execute actions necessary to manage prospective situations. This belief significantly influences how individuals approach goals, tasks, and challenges across different domains of life.Psychological and Educational ImpactsIndividuals with strong self-efficacy are more resilient in the face of difficulties. They are more likely to adopt effective problem-solving strategies, persist through obstacles, and regulate emotions such as...
03:44
Luciferase Assay to Study Efficacy of Antiparasitic Chemicals Against Parasites
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2025
The video demonstrates the luciferase assay used to determine the efficacy of an antiparasitic chemical inhibitor against the intracellular pathogen Toxoplasma gondii. The study involves luciferase-expressing parasites invading the host cells to replicate and survive. The chemical inhibitor binds with the cysteine proteases of the parasite and inhibits replication. The luciferase activity detected is proportional to the fold changes in parasite growth, determining the drug's efficacy against...
09:44
High-throughput Screening for Chemical Modulators of Post-transcriptionally Regulated Genes
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2015
Here we describe a cell-based reporter gene assay as a valuable tool to screen chemical libraries for compounds modulating post-transcriptional control mechanisms exerted through 3’ UTR.
FAQs
These formulation features can alter particle stability, cargo loading, release behavior, cellular uptake, and delivery to the intended site. Environmental conditions may further change performance during testing. Comparing formulations under controlled conditions helps bioengineers distinguish effects caused by nanocarrier design from effects caused by the surrounding experimental environment, supporting more informed optimization and selection.
Cargo release and cellular uptake describe different stages of delivery performance. A formulation may enter cells efficiently yet release its cargo inadequately, or release cargo effectively without achieving sufficient uptake. Measuring both outcomes provides a more complete view of nanocarrier function and helps identify which stage limits the desired therapeutic or diagnostic response.
Target-site delivery evaluates whether the nanocarrier reaches the intended cells or tissues, whereas biological response measures the consequence of that delivery. These outcomes should be interpreted together because successful localization does not by itself establish effectiveness. Combining delivery measurements with response data helps connect nanocarrier distribution to therapeutic or diagnostic performance.
In vitro and in vivo evaluations provide complementary evidence under controlled conditions. In vitro assays can support direct comparisons of stability, cargo behavior, uptake, and cellular responses, while in vivo studies examine performance in an organism and can assess delivery to intended tissues. Using both settings strengthens formulation selection before translation toward biomedical use.
A screening workflow can begin by comparing particle stability and cargo loading, followed by measurements of cargo release, cellular uptake, target-site delivery, and biological response. Testing formulations under controlled in vitro or in vivo conditions allows results to be compared consistently. The resulting performance profile helps identify candidates for further optimization rather than relying on a single assay.
The approach supports formulation decisions for drug delivery, gene therapy, and imaging by revealing how engineered particles transport different types of cargo and affect biological outcomes. It also contributes to evaluating safety alongside performance. In bioengineering, these comparisons help move promising nanomedicines toward more reliable biomedical use and eventual translation.