Embryo Yolk Injection
Embryo Yolk Injection - Related Videos
09:47
AFM and Microrheology in the Zebrafish Embryo Yolk Cell
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Cited by 3 •
2017
The lack of tools to measure material properties and tensional parameters in vivo prevents validating their roles during development. We employed atomic force microscopy (AFM) and nanoparticle-tracking to quantify mechanical features on the intact zebrafish embryo yolk cell during epiboly. These methods are reliable and widely applicable avoiding intrusive interventions.
03:58
Embryo Microinjection: A Technique to Deliver a Compound into the Zebrafish Yolk
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2023
This video describes a technique of Embryo microinjection to deliver compounds into Zebrafish yolk sac.
03:12
Mosquito Embryo Microinjection: A Technique to Deliver Exogenous DNA into Embryo Yolk of Anopheles Gambiae to Generate Germline Mutations
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2025
In this video, we demonstrate the delivery of gene drive construct into the embryo of Anopheles gambiae mosquito through embryo microinjection technique. This procedure allows for integration of delivered DNA construct, through homologous recombination, into the germline cells to generate transformed mosquito population.
11:53
Primary Endodermal Epithelial Cell Culture from the Yolk Sac Membrane of Japanese Quail Embryos
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Cited by 6 •
2016
To study the mechanism of lipid utilization in yolk sac membranes during the late stages of avian embryonic development, we established a primary Japanese quail embryonic endodermal epithelial cell culture system.
06:10
Injection of Microbubbles into Isolated Mouse Embryos: A Technique to Deliver Microbubble Contrast Agents into the Vasculature of Living Murine Embryos
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2025
This video demonstrates a procedure to inject microbubble contrast agents into an isolated late gestation stage mouse embryo. The microbubble contrast agent-based ultrasound imaging helps visualize and characterize the vascular environment of the embryo.
FAQs
After deposition, the test material can diffuse through the yolk toward embryonic tissues as development proceeds. This creates a route for early developmental exposure without requiring direct placement into embryonic structures. The resulting contact allows investigators to examine how a defined substance may influence growth or other developmental responses over time.
These variables determine how consistently embryos encounter the introduced material. Standardizing when injection occurs, how much is delivered, and where it is deposited in the yolk helps researchers compare embryos and relate developmental outcomes to the test substance rather than to differences in exposure conditions. This consistency is especially important when assessing toxicity or experimental treatments.
The yolk provides an accessible site for introducing test materials during early development. From there, substances can move toward embryonic tissues while the embryo continues developing. This arrangement supports controlled exposure studies and can reduce the need for more invasive procedures, making it useful for examining developmental effects in egg-based biological models.
Researchers use a fine needle to penetrate the egg and deposit the selected cells, molecules, or other test material into the yolk. The injection is planned around a defined developmental time, volume, and placement so exposure can be compared across embryos. Subsequent observations can then relate those conditions to developmental outcomes.
The technique can support investigations of embryonic growth, nutrient transfer, developmental toxicity, and experimental treatments. Because the introduced material reaches embryonic tissues as development proceeds, researchers can examine how exposure relates to developmental changes. Controlled timing, volume, and placement strengthen comparisons among embryos receiving different test materials or conditions.
Embryo yolk injection can expose developing embryos to a specified test substance under controlled conditions. Researchers can then assess whether development changes after exposure, using consistent timing, volume, and placement to improve comparisons. This makes the method relevant to developmental toxicity studies as well as investigations of experimental treatments.