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Primary Endodermal Epithelial Cell Culture from the Yolk Sac Membrane of Japanese Quail Embryos
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Developmental Biology
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JoVE Journal Developmental Biology
Primary Endodermal Epithelial Cell Culture from the Yolk Sac Membrane of Japanese Quail Embryos

Primary Endodermal Epithelial Cell Culture from the Yolk Sac Membrane of Japanese Quail Embryos

DOI:
10.3791/53624-v

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11:53 min

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March 10, 2016

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, , ,
1Department of Animal Science and Technology,National Taiwan University

Chapters

  • 00:05Title
  • 01:03Preparation of Basal Medium and Wash Solutions
  • 01:57Collection of Primary Endodermal Epithelium from Yolk Sac Membranes
  • 05:06Digestion of Endoderm by Collagenase
  • 08:26Results: Culture System Performance and its Application to Study Lipid Utilization
  • 10:30Conclusion

Summary

Automatic Translation

Automatic Translation

To study the mechanism of lipid utilization in yolk sac membranes during the late stages of avian embryonic development, we established a primary Japanese quail embryonic endodermal epithelial cell culture system.

Tags

Avian Endodermal Epithelial CellsYolk Sac MembraneJapanese Quail EmbryosEx Vivo Explant ProcedureNutrient UtilizationLipid UtilizationYolk LipidsAvian EmbryogenesisFunctional Endodermal Epithelial CellsAvian Embryonic Development10x PBS SolutionGrowth MediumEECsDMEM/F-12Newborn Calf SerumPenicillin Streptomycin Amphotericin SolutionPH MeterHCl AdjustmentFertilized Quail Eggs

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