Equine Cloning
Equine Cloning - Related Videos
04:36
A Simple Microaspiration Technique for Isolating Somatic Cells from Cryopreserved Equine Semen as Nuclear Donors for Cloning
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2025
This protocol presents a simple microaspiration technique to isolate viable somatic cells (SCs) from cryopreserved equine semen. Unlike traditional approaches, this technique avoids long in vitro cultures and overcomes sperm contamination, enabling immediate use in somatic cell cloning.
09:55
Molecular Cloning
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2023
Molecular cloning is a set of methods, which are used to insert recombinant DNA into a vector - a carrier of DNA molecules that will replicate recombinant DNA fragments in host organisms. The DNA fragment, which may be a gene, can be isolated from a prokaryotic or eukaryotic specimen. Following isolation of the fragment of interest, or insert, both the vector and insert must be cut with restriction enzymes and purified. The purified pieces are joined together though a technique called...
09:41
Surgical Closure of Equine Abdomen, Prevention, and Management of Incisional Complications
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Cited by 4 •
2024
This paper describes a method of abdominal wall closure using tension and apposition sutures in horses undergoing ventral midline laparotomies. It also describes methods for prevention and management of postoperative incisional complications, such as bandaging, negative pressure therapy, and in case of wound breakdown, the application of retention sutures.
05:21
Culturing and Harvesting of HSPC Clones: A Method to Obtain the Singly-Sorted HSPC Clones
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2023
This video describes the protocol for isolating HSPCs from the bone marrow and sorting them using a fluorescent activated cell sorting technique, which is a method to obtain singly-sorted HSPCs. We culture these sorted cells to obtain HSPC clones.
04:25
Harvesting Articular Cartilage From Joints of an Equine Donor: A Surgical Procedure to Isolate Vascular Connective Tissue From Cadaveric Joints of an Equine Donor
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2025
In this video, we demonstrate the extraction of articular cartilage from an equine cadaveric joint. The isolated articular cartilage can be used for further downstream analysis.
FAQs
Removing the egg cell’s nucleus prevents its nuclear DNA from contributing to the reconstructed embryo. This allows the somatic cell donor to provide the nuclear genetic material that determines the foal’s genetic match. The distinction is important because the egg still contributes cellular contents, including mitochondrial DNA, so the resulting animal is not identical in every genetic component.
Activation provides the stimulus needed for the reconstructed egg to begin dividing as an embryo. Without this electrical or chemical signal, the transferred nucleus and enucleated egg would not proceed through the early developmental process required for implantation. In this way, activation links the laboratory reconstruction step to embryo formation and subsequent gestation.
A cloned horse shares the donor’s nuclear DNA, but its mitochondrial DNA can differ because mitochondria are associated with the egg cell rather than solely with the transferred somatic nucleus. Developmental effects can also influence the outcome. Consequently, cloning produces a genetically matched horse at the nuclear level, not a guaranteed duplicate in every biological characteristic.
The process creates a biological system in which a somatic-cell nucleus is placed in an enucleated egg and then stimulated to divide. Observing this transition helps biology and veterinary researchers examine early embryonic development in horses. The work connects nuclear genetic material, egg-cell activity, activation, and embryo formation within a single experimental context.
The procedure begins with a somatic cell and an egg cell whose nucleus has been removed. After nuclear transfer, electrical or chemical activation stimulates the reconstructed egg to divide into an embryo. Researchers then implant that embryo into a surrogate mare, where gestation can produce the resulting foal. Each stage is necessary for development to continue.
Equine cloning can support preservation of valuable genetic material and replication of elite or sterile animals. This makes the approach relevant when a particular horse’s nuclear genetics are considered especially important for biological, veterinary, or breeding-related purposes. The method therefore extends beyond developmental research to practical efforts involving selected equine genetics.
Equine cloning raises questions about efficiency, animal welfare, and genetic diversity. These concerns place the technical procedure within a broader biological and veterinary context: researchers must consider how reliably the approach produces outcomes, how the animals involved are affected, and whether concentrating on genetically matched animals could limit diversity within horse populations.