Fluorescent Rna
Fluorescent Rna - Related Videos
09:57
Whole Mount RNA Fluorescent in situ Hybridization of Drosophila Embryos
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Cited by 22 •
2013
Here we describe a whole-mount fluorescent in situ hybridization (FISH) protocol for determining the expression and localization properties of RNAs expressed during embryogenesis in the fruit fly, Drosophila melanogaster.
10:16
Detection of Viral RNA by Fluorescence in situ Hybridization (FISH)
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Cited by 35 •
2012
A fluorescence in situ hybridization (FISH) method was developed to visually detect viral genomic RNA using fluorescence microscopy. A probe is made with specificity to the viral RNA that can then be identified using a combination of hybridization and immunofluorescence techniques. This technique offers the advantage of identifying the localization of the viral RNA or DNA at steady-state, providing information on the control of intracellular virus trafficking events.
02:59
Using Immuno-RNA Fluorescence In Situ Hybridization to Visualize SARS-CoV-2
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2025
This video demonstrates the application of Hybridization Chain Reaction (HCR)-Fluorescence In Situ Hybridization (FISH) for visualizing the spatial distribution of SARS-CoV-2 subgenomic RNA. Combining HCR with immunofluorescence techniques has the potential to provide insights into host-virus interactions at the single-cell level.
04:23
Determining Viral RNA Distribution in Host Cells by Fluorescence In Situ Hybridization
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2026
Source: Vallery, T. K. & Steitz, J. A. Quantitative Fluorescence In Situ Hybridization (FISH) and Immunofluorescence (IF) of Specific Gene Products in KSHV-Infected Cells. J. Vis. Exp. (2019)This video demonstrates fluorescence in situ hybridization to visualize viral RNA in virus-infected cells. It outlines the steps involved in probe hybridization, signal amplification, and confocal imaging.
05:34
Denaturing Urea Polyacrylamide Gel Electrophoresis for RNA Analysis: A Technique to Separate Fluorescently Labeled Phosphorylated RNA Oligonucleotides from their Non-Phosphorylated Equivalents
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2025
In this video, we demonstrate a denaturing polyacrylamide gel electrophoresis for the separation of phosphorylated RNA species from their non-phosphorylated counterparts. The RNA species are fluorescently labeled for laser detection on the gel.
FAQs
The aptamer folds into a structure that binds a fluorogenic small molecule. Binding restricts the dye’s motion, which increases emitted light compared with the unbound state. This structural coupling converts RNA folding and ligand interaction into an optical signal that can be monitored in biological systems.
Dye attachment makes RNA visible by physically associating a fluorescent label with the molecule. Sequence-based approaches instead incorporate RNA sequences that activate fluorescence, commonly through aptamer folding and small-molecule binding. These alternatives provide different ways to connect RNA presence or behavior with an observable fluorescent signal.
The RNA must adopt a structure capable of binding the fluorogenic molecule effectively. That interaction is important because it restricts the dye’s motion and increases emitted light. Consequently, the RNA’s folding behavior and its interaction with the small molecule influence how clearly researchers can observe the fluorescent signal.
A study first uses either a fluorescent dye attached to RNA or an RNA sequence that activates fluorescence. In the latter strategy, a fluorogenic small molecule binds the folded RNA structure. Researchers can then monitor fluorescence to examine RNA location, movement, abundance, or interactions in a biological system.
Fluorescent RNA can reveal where RNA is located, how it moves, and how its abundance changes. These observations support studies of gene expression, RNA transport, and RNA processing. Fluorescent readouts can also help examine interactions between RNA and proteins or between RNA and other nucleic acids.
Using these tools in living cells connects RNA behavior with cellular function, while cell-free systems provide another setting for examining RNA-related processes. Across both contexts, fluorescence supports observation of RNA location, movement, abundance, processing, and molecular interactions, contributing to research in molecular biology, development, and disease mechanisms.