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JoVE Journal
Biology

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Detection of Viral RNA by Fluorescence in situ Hybridization (FISH)
 

Detection of Viral RNA by Fluorescence in situ Hybridization (FISH)

Article doi: 10.3791/4002
May 5th, 2012

Summary May 5th, 2012

A fluorescence in situ hybridization (FISH) method was developed to visually detect viral genomic RNA using fluorescence microscopy. A probe is made with specificity to the viral RNA that can then be identified using a combination of hybridization and immunofluorescence techniques. This technique offers the advantage of identifying the localization of the viral RNA or DNA at steady-state, providing information on the control of intracellular virus trafficking events.

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