Laser Ablation
Laser Ablation - Related Videos
07:27
Laser Ablation of the Zebrafish Pronephros to Study Renal Epithelial Regeneration
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Cited by 43 •
2011
Acute kidney injury (AKI) in humans is a common clinical problem caused by damage to the epithelial cells that comprise kidney nephrons, and AKI is associated with high mortality rates of 50-70%1. Following epithelial cell destruction, nephrons have a limited ability to regenerate, though the mechanisms and limitations that guide this phenomenon remain poorly understood. In this video article, we describe our technique for targeted laser ablation of kidney nephron cells in the zebrafish...
05:13
Selective Single-Neuron Laser Ablation in a Zebrafish Larva Using a Confocal Microscope
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2025
Source: Morsch, M., et. al., Triggering Cell Stress and Death Using Conventional UV Laser Confocal Microscopy. J. Vis. Exp. (2017) In this video, we explore the technique of high-intensity UV laser ablation for precisely targeting and studying individual neurons in transgenic zebrafish. We demonstrate how to prepare, immobilize, and visualize neuronal responses, highlighting the cellular dynamics of injury and recovery.
02:39
Monitoring Recovery of Laser-Ablated Interneuromast Cells in Zebrafish Using Confocal Microscopy
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2025
Source: Volpe, B. A., et al. Confocal Microscope-Based Laser Ablation and Regeneration Assay in Zebrafish Interneuromast Cells. J. Vis. Exp. (2020) This video demonstrates monitoring interneuromast cell recovery in zebrafish using confocal microscopy, tracking cell death and repair through time-lapse fluorescence imaging.
01:20
Laser Interstitial Thermal Therapy for Intracranial Tumor Ablation in a Human Patient
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2025
Begin with an anesthetized human patient with an exposed skull. The patient is diagnosed with an intracranial tumor.Drill into the exposed skull to create a hole, generating an access point.Using stereotactic guidance, insert a laser diffusing fiber or LDF along the predetermined path and secure it in place.Transfer the patient to an MRI scanner and connect the LDF to a laser source.Capture MRI images from multiple angles to visualize the fiber path and the tumor.This confirms the LDF’s...
13:02
Fabrication of 1-D Photonic Crystal Cavity on a Nanofiber Using Femtosecond Laser-induced Ablation
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Cited by 3 •
2017
We present a protocol for fabricating 1-D photonic crystal cavities on subwavelength diameter silica fibers (optical nanofibers) using femtosecond laser-induced ablation.
FAQs
These parameters determine how laser energy interacts with the target. Their combination can favor photothermal heating, photochemical change, or photomechanical disruption, while also influencing whether material is removed, vaporized, or otherwise modified. Adjusting them allows researchers to match the treatment to a cell, tissue region, or molecular sample and limit effects beyond the intended target.
Photothermal effects arise when absorbed energy produces heating, photochemical effects involve laser-driven chemical changes, and photomechanical effects disrupt material through mechanical action. The dominant process depends on the laser conditions and the target. Recognizing this distinction helps researchers interpret whether an experiment primarily modifies molecular material, damages cellular structures, or removes tissue through localized energy deposition.
Focusing concentrates energy within a selected spatial region, supporting precise modification of cells, tissue, or molecular material. This localization is important because biological structures often lie close together, so unwanted effects in surrounding regions could obscure the result. Spatially restricted treatment therefore helps connect a targeted removal or disruption with the biological response that follows.
Researchers can remove selected cells or structures and then examine how the surrounding system responds. In cell microsurgery, this approach supports studies of cell function, while targeted removal in tissues and developing organisms can reveal relationships among structures and organization. The resulting changes provide experimental information about how biological regions contribute to broader tissue or developmental processes.
A biological sample is positioned so the laser can be focused on the selected cell, tissue region, structure, or molecular material. Researchers then choose wavelength, pulse duration, and energy according to the intended interaction, apply the focused treatment, and assess the resulting removal or modification. This workflow supports precise intervention while reducing the need for direct physical contact.
The technique is useful when a biological sample must be selectively removed or modified before analysis of its elemental or molecular composition. Focused treatment can target a defined region, helping prepare material for subsequent characterization while preserving spatial information about where the sample originated. This application extends the method beyond microsurgery into analytical preparation and biological measurement.