Leaf Infiltration
Leaf Infiltration - Related Videos
11:50
Bacterial Leaf Infiltration Assay for Fine Characterization of Plant Defense Responses using the Arabidopsis thaliana-Pseudomonas syringae Pathosystem
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Cited by 47 •
2015
Quantification of pathogen growth is a powerful tool to characterize various Arabidopsis thaliana (hereafter: Arabidopsis) immune responses. The method described here presents an optimized syringe infiltration assay to quantify the Pseudomonas syringae pv. maculicola ES4326 growth in adult Arabidopsis leaves.
03:03
Inoculating a Bacterial Pathogen into Arabidopsis Leaves via Vacuum Infiltration
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2026
Source: Rufián, J. S., et.al. Single-Cell Analysis of the Expression of Pseudomonas syringae Genes within the Plant Tissue. J. Vis. Exp. (2022)This video demonstrates a vacuum infiltration method to introduce a bacterial pathogen into Arabidopsis plant leaves. The approach uses a prepared bacterial suspension and vacuum pulses to enable uniform entry through stomata, resulting in controlled leaf infection.
09:56
A Stepwise Guide to the Isolation and Analysis of Leaf Surface and Apoplastic RNA Using Arabidopsis Rosettes
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2025
We describe a simple yet highly reproducible method to isolate extracellular RNA from the leaf surface and apoplast of Arabidopsis plants.
11:09
Relating Stomatal Conductance to Leaf Functional Traits
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Cited by 39 •
2015
Unraveling how physiology and morphology are linked allows a deeper understanding of mechanistic functioning of plant leaves. We present both a procedure to derive parameters of stomatal regulation from stomatal conductance measurements and correlations with traditional functional leaf traits.
06:44
Isolation of Brain-infiltrating Leukocytes
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Cited by 33 •
2011
A rapid method to obtain infiltrating leukocytes from the murine brain is described. This method utilizes a continuous Percoll gradient and discontinuous Ficoll gradient to select and purify the leukocyte-enriched layer. Isolated leukocytes may then be characterized by flow cytometric measurements.
FAQs
The key physical step is pressure-driven displacement of air from the leaf’s intercellular spaces. A syringe or vacuum pushes the treatment suspension into these internal spaces, bringing its contents into direct proximity with leaf tissue. This localized entry helps researchers examine responses at the treated site and maintain a controlled contrast with untreated parts of the plant.
In agroinfiltration, Agrobacterium serves as the biological mediator for transient gene expression. The introduced suspension carries the bacterial cells into leaf tissue, where the experiment can assess gene activity without permanently altering the plant genome. This temporary expression is useful when investigators need a rapid readout of gene function or production of a target protein.
The liquid formulation determines what experimental material reaches the leaf. Depending on the study, the suspension may contain cells, molecules, or microorganisms, so infiltration can test biological interactions as well as deliver a material for expression or response assays. The selected contents therefore connect the physical entry step to the specific biological question being investigated.
A basic workflow begins by preparing a liquid suspension suited to the experiment, then applying gentle pressure with a syringe or vacuum so the solution enters the leaf’s internal spaces. Researchers subsequently examine the local plant response or the effect of the delivered material. This sequence supports controlled experiments because treatment is confined to selected leaf tissue.
Leaf infiltration is especially useful when the goal is a localized, rapid experiment, such as testing gene function, producing a protein, or examining a disease response. The method allows investigators to evaluate treatment effects in leaf tissue while avoiding permanent alteration of the plant genome. Its local format also supports controlled comparisons among experimental treatments.
In plant biology, the technique connects experimental delivery with plant–microbe research. Investigators can introduce microorganisms or Agrobacterium-containing suspensions and then study how leaf tissue responds, including responses associated with disease. Because the treatment is local and results are obtained quickly, infiltration helps compare defined experimental conditions while focusing analysis on the treated tissue.