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Bacterial Leaf Infiltration Assay for Fine Characterization of Plant Defense Responses using the Arabidopsis thaliana-Pseudomonas syringae Pathosystem
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Immunology and Infection
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JoVE Journal Immunology and Infection
Bacterial Leaf Infiltration Assay for Fine Characterization of Plant Defense Responses using the Arabidopsis thaliana-Pseudomonas syringae Pathosystem

Bacterial Leaf Infiltration Assay for Fine Characterization of Plant Defense Responses using the Arabidopsis thaliana-Pseudomonas syringae Pathosystem

DOI:
10.3791/53364-v

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11:50 min

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October 01, 2015

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, , , , ,
1Department of Biology,University of Alabama at Birmingham

Chapters

  • 00:05Title
  • 01:53Preparations Prior to Infiltration
  • 03:40Syringe Infiltration
  • 05:57Sampling Leaf Tissue and Quantifying Pathogen Growth
  • 09:11Results: Syringe Infiltration Assays can Quantitatively Assess Plant Immune Response
  • 10:33Conclusion

Summary

Automatic Translation

Automatic Translation

Quantification of pathogen growth is a powerful tool to characterize various Arabidopsis thaliana (hereafter: Arabidopsis) immune responses. The method described here presents an optimized syringe infiltration assay to quantify the Pseudomonas syringae pv. maculicola ES4326 growth in adult Arabidopsis leaves.

Tags

Keywords: Bacterial Leaf InfiltrationArabidopsis ThalianaPseudomonas SyringaePathogen Growth AssaySystemic Acquired ResistanceProgrammed Cell DeathSyringe InfiltrationEnhanced Disease SusceptibilitySalicylic Acid-triggered ImmunityMAMP-triggered Immunity

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