Microfluidic Cassette
Microfluidic Cassette - Related Videos
10:35
Studies of Bacterial Chemotaxis Using Microfluidics - Interview
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Cited by 1 •
2007
11:03
Pouring and Running a Protein Gel by reusing Commercial Cassettes
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Cited by 2 •
2012
Our protocol demonstrates how to pour multiple protein gels at a time by recycling Invitrogen Nupage Novex minigel cassettes, and inexpensive materials purchased at a home improvement store. This economical and streamlined method includes a way to store the gels at 4°C for a few weeks. By re-using the plastic gel cassettes from commercially available gels, labs that run frequent protein gels can save significant costs and help the environment.
09:37
Screening Foodstuffs for Class 1 Integrons and Gene Cassettes
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Cited by 13 •
2015
This protocol describes the detection of class 1 integrons and their associated gene cassettes in foodstuffs.
10:18
Multi-step Variable Height Photolithography for Valved Multilayer Microfluidic Devices
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Cited by 26 •
2017
Multilayer microfluidic devices often involve the fabrication of master molds with complex geometries for functionality. This article presents a complete protocol for multi-step photolithography with valves and variable height features tunable to any application. As a demonstration, we fabricate a microfluidic droplet generator capable of producing hydrogel beads.
05:41
Applying Microfluidics to Electrophysiology
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Cited by 1 •
2007
Microfluidics can be integrated with standard electrophysiology techniques to allow new experimental modalities. Specifically, the motivation for the ...
FAQs
These driving forces determine how samples and reagents travel through the enclosed channel network. Pressure can propel fluids, capillary action can guide movement without the same type of applied force, and external control can regulate flow when coordinated handling is needed. Selecting among these mechanisms helps direct fluids through mixing, separation, and reaction zones in the intended sequence.
Each zone performs a distinct fluid-handling or analytical role within the cassette. Mixing brings samples and reagents into contact, separation helps manage different components, and reaction zones support the biological analysis itself. Keeping these functions in defined regions allows the device to coordinate processing in a compact format while using very small sample volumes.
The enclosed format helps limit contamination during biological processing and supports more consistent handling of samples and reagents. It also allows fluid movement and analytical steps to remain within a controlled miniature network rather than requiring separate open transfers. These characteristics can reduce reagent use while supporting automation, portability, and reproducibility across workflows.
A typical workflow places the biological sample and required reagents into the cassette, then directs their movement through the enclosed channels using pressure, capillary action, or external control. The fluids pass through selected mixing, separation, or reaction regions, where processing occurs. The resulting analysis can then support applications such as nucleic acid testing, immunoassays, or cell handling.
Microfluidic cassettes support several distinct biological workflows, including cell handling, nucleic acid analysis, immunoassays, and rapid diagnostic testing. Their integrated fluid paths can coordinate sample and reagent contact for these uses while limiting reagent consumption. This range makes the format relevant to both research workflows and development of compact analytical systems.
Their compact, often disposable format supports portability and can simplify biological analysis outside a large laboratory setting. Enclosed fluid handling may reduce contamination, while integrated channels and chambers can improve automation and consistency. These features are particularly relevant to point-of-care testing, where rapid diagnostic analysis and efficient use of small sample and reagent volumes are important.