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Screening Foodstuffs for Class 1 Integrons and Gene Cassettes
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JoVE Journal Environment
Screening Foodstuffs for Class 1 Integrons and Gene Cassettes

Screening Foodstuffs for Class 1 Integrons and Gene Cassettes

DOI:
10.3791/52889-v

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09:37 min

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June 19, 2015

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,
1Department of Biological Sciences,Macquarie University

Chapters

  • 00:05Title
  • 01:42Enriched Culture Preparation
  • 02:54Single Colony Selection
  • 04:38Screening Single Colonies for Class 1 Integrons
  • 05:44Diagnostics PCRs and DNA Sequencing
  • 07:35Results: Culturing, PCR, and DNA Sequencing Can Identify Class 1 Integrons and Gene Cassettes
  • 09:13Conclusion

Summary

Automatic Translation

Automatic Translation

This protocol describes the detection of class 1 integrons and their associated gene cassettes in foodstuffs.

Tags

Keywords: Antibiotic ResistanceLateral Gene TransferClass 1 IntegronsGene CassettesFoodstuffsPCRBacterial CulturePathogenCommensalsHuman WasteFood Chain

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