Mouse Skin Wound
Mouse Skin Wound - Related Videos
09:20
A Mouse Fetal Skin Model of Scarless Wound Repair
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Cited by 24 •
2015
During mammalian development, early gestational skin wounds heal without a scar. Here we detail a reliable and reproducible model of fetal scarless wound healing in the cutaneous dorsum of E16.5 (scarless) and E18.5 (scarring) mouse embryos.
08:35
Demonstration of the Rat Ischemic Skin Wound Model
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Cited by 32 •
2015
The rat, due to its size, availability, and rather docile behavior, has been utilized as a research model for many years. The goal of this protocol is to utilize the rat as an ischemic skin wound healing model to provide valuable insight into the pathophysiology of chronic wounds.
02:20
Generating an Excisional Skin Wound in a Mouse Model via Punch Biopsy
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2025
This video demonstrates a punch biopsy procedure to create a precise wound in a mouse model. The excision exposes the subcutaneous layer of the skin, providing a suitable site for studying immune responses to localized pathogen infection.
02:24
Developing a Mouse Model of Chronic Pseudomonas aeruginosa Skin Wound Infection
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2026
Source: de Vries, C. R., et.al. A Delayed Inoculation Model of Chronic Pseudomonas aeruginosa Wound Infection. J. Vis. Exp. (2020)This video demonstrates the delayed inoculation of luminescent Pseudomonas aeruginosa into a mouse bearing two full-thickness dorsal skin wounds covered by a transparent film dressing. This facilitates biofilm formation and the establishment of chronic infection. Regular bioluminescence monitoring enables quantification of bacterial burden and tracking of infection...
08:35
Generation of a Three-dimensional Full Thickness Skin Equivalent and Automated Wounding
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Cited by 53 •
2015
The goal of this protocol is to build up a three-dimensional full thickness skin equivalent, which resembles natural skin. With a specifically constructed automated wounding device, precise and reproducible wounds can be generated under maintenance of sterility.
FAQs
Healing proceeds through coordinated phases rather than isolated events. Hemostasis first limits blood loss, followed by immune-cell activity that removes damaged tissue and microbes. Keratinocytes then migrate over the injured area, while fibroblasts deposit extracellular matrix and new blood vessels support granulation tissue. Contraction and remodeling subsequently strengthen and restore the tissue.
Each component contributes a different requirement for tissue restoration. Keratinocyte migration helps cover the wound surface, fibroblast production of extracellular matrix provides structural support, and new blood vessels sustain granulation tissue. Examining these coordinated activities allows researchers to connect surface closure, matrix formation, and tissue support with the overall quality of healing.
Excisional and incisional models represent different forms of controlled skin injury, allowing investigators to examine repair under distinct wound configurations. Genetically modified mice add biological variation by altering the animal's genetic context. Together, these models support comparisons between normal and impaired healing and help identify pathways that influence tissue repair and regeneration.
Investigators can compare wound repair in different mouse groups, including standard animals and genetically modified models. Differences in inflammation, keratinocyte migration, extracellular matrix production, blood-vessel support, contraction, or remodeling may reveal which healing processes are disrupted. This comparison provides a way to connect altered biological pathways with impaired tissue recovery.
These experiments can provide mechanistic information about tissue repair, inflammation, and regeneration. They may also show how the major repair activities progress together, from immune clearance and surface coverage to matrix formation, vascular support, contraction, and remodeling. Such outcomes help researchers evaluate whether a treatment or biomaterial influences the restoration of tissue integrity.
Biologists use these models to investigate healing pathways and to evaluate potential drugs, biomaterials, and cell-based therapies. They are especially useful when researchers need a controlled mammalian system for comparing repair responses or studying impaired healing. Findings can provide mechanistic insight into wound repair and may help inform approaches for chronic wounds.