The goal of this protocol is to build up a three-dimensional full thickness skin equivalent, which resembles natural skin. With a specifically constructed automated wounding device, precise and reproducible wounds can be generated under maintenance of sterility.
Rossi, A., Appelt-Menzel, A., Kurdyn, S., Walles, H., Groeber, F. Generation of a Three-dimensional Full Thickness Skin Equivalent and Automated Wounding. J. Vis. Exp. (96), e52576, doi:10.3791/52576 (2015).