Ocean Acidification

Ocean acidification is the ongoing decrease in seawater pH caused primarily by the ocean’s absorption of excess atmospheric carbon dioxide, making it an important biological and environmental process. Dissolved CO2 reacts with water to form carbonic acid, which releases hydrogen ions and reduces the availability of carbonate ions needed by many marine organisms to build calcium carbonate shells and skeletons. In biology, ocean acidification research examines effects on corals, mollusks, plankton, fish physiology, and marine food webs. Understanding these responses supports ecosystem monitoring, climate-change assessment, and strategies for protecting marine biodiversity and fisheries.

Ocean Acidification - Related Videos

Research

JoVE EoE - Assay Techniques

Gut Acidification Monitoring Assay: A Technique to Study the Acidification of the Intestinal Lumen in Drosophila using Bromophenol Blue Dye

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2025

This video demonstrates an assay to monitor gut acidification in Drosophila flies. After the ingestion of food supplemented with a pH indicator dye, the acidification of the intestinal lumen results in a change in the color of the dye. Visual inspection of the digestive tract of the flies to examine the color change provides evidence of acidification.

Monitoring Gut Acidification in the Adult Drosophila Intestine

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Cited by 10 •

2021

Here, we present a standardized protocol for monitoring gut acidification in Drosophila melanogaster with optimal output. We first use this protocol for gut acidification monitoring in Drosophila melanogaster and then demonstrate its use in non-model Drosophila species.

Research

JoVE Journal - Environment
Free Sample

Unraveling the Unseen Players in the Ocean - A Field Guide to Water Chemistry and Marine Microbiology

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Cited by 33 •

2014

Here, we present a comprehensive protocol to assess the organic and inorganic nutrient availability and the abundance and structure of microbial and viral communities in remote marine environments.

Clean Sampling and Analysis of River and Estuarine Waters for Trace Metal Studies

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Cited by 3 •

2016

Special care using "clean techniques" is required to properly collect and process water samples for trace metal studies in aquatic environments. A protocol for sampling, processing, and analytical procedures with the aim of obtaining reliable environmental monitoring data and results with high sensitivity for detailed trace metal studies is presented.

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