A subscription to JoVE is required to view this content. Sign in or start your free trial.
Chapters
- 00:05Introduction
- 00:58Preparing for the Gut Acidification Monitoring Assay
- 01:54Gut Acidification Monitoring Assay
- 02:30Mounting and Image Acquisition
- 02:52Results: Gut Acidification Monitoring and Phylogeny of Gut Acidification Phenomenon
- 03:56Conclusion
Here, we present a standardized protocol for monitoring gut acidification in Drosophila melanogaster with optimal output. We first use this protocol for gut acidification monitoring in Drosophila melanogaster and then demonstrate its use in non-model Drosophila species.
