Recombinant Adenovirus
Recombinant Adenovirus - Related Videos
13:47
Efficient Recombinant Parvovirus Production with the Help of Adenovirus-derived Systems
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Cited by 8 •
2012
Here we describe a protocol based only on cell infection, which improves the efficiency of recombinant parvovirus production by more than 100 fold in comparison to other protocols in use. This protocol relies on the use of a novel adenovirus 5-based helper containing the parvovirus VP transcription unit (Ad-VP).
02:40
Detecting SMAD Pathway Activation Using Dual Adenovirus Transduction
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2026
Source: Chen, H., et al. Live Cell Imaging of the TGF-β/Smad3 Signaling Pathway In Vitro and In Vivo Using an Adenovirus Reporter System. J. Vis. Exp. (2018).This video demonstrates a method to study SMAD pathway activation in breast cancer cells using dual adenoviral infection. One virus delivers a constitutively expressed GFP gene, while the other carries an RFP reporter under a SMAD-sensitive promoter. Upon TGF-beta stimulation, SMAD proteins are activated, forming a complex that induces RFP...
11:13
Dissection of Adult Mouse Utricle and Adenovirus-mediated Supporting-cell Infection
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Cited by 17 •
2012
Mechanosensory hair cells are the receptor cells of the inner ear. The best-characterized in vitro model system of mature mammalian hair cells utilizes organ cultures of utricles from adult mice. We present the dissection of the adult mouse utricle, and we demonstrate adenovirus-mediated infection of supporting cells in cultured utricles.
13:36
Utilizing the Antigen Capsid-Incorporation Strategy for the Development of Adenovirus Serotype 5-Vectored Vaccine Approaches
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Cited by 1 •
2015
Here, we present a protocol to generate a proof-of-principle divalent adenovirus type 5 (Ad5) vector Ad5/H5-HVR1-KWAS-HVR5-His6 by utilizing the Antigen Capsid-Incorporation strategy. This vector was demonstrated to exhibit qualitative fitness, the capability to escape Ad5-positive sera in vitro, and the antigenicity as well as immunogenicity to the incorporated antigens.
14:23
Recombineering Homologous Recombination Constructs in Drosophila
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Cited by 7 •
2013
Homologous recombination techniques greatly advance Drosophila genetics by enabling the creation of molecularly precise mutations. The recent adoption of recombineering allows one to manipulate large pieces of DNA and transform them into Drosophila6. The methods presented here combine these techniques to rapidly generate large homologous recombination vectors.
FAQs
After entering a mammalian cell, the vector uses its engineered genetic payload to direct production of the selected protein. This makes it useful for examining what a gene product does or for testing whether expression changes a cellular response. Because delivery can occur in both dividing and nondividing cells, experiments are not limited to one cell-growth state.
Removing adenoviral genes required for replication changes the vector’s role from a self-propagating virus to a delivery vehicle for an inserted transgene. The engineered particle can still enter target mammalian cells and support encoded-protein production, but replication-dependent functions have been removed. This design is central to controlled gene-expression studies.
Recombinant adenovirus generally delivers its genetic material without inserting it into the host genome. That distinguishes it from approaches designed around genomic integration, while preserving the ability to express the inserted sequence in mammalian cells. The distinction matters when researchers need delivered gene activity without typically making the vector sequence a permanent part of host DNA.
These vectors strongly stimulate host immune responses, which can be advantageous for vaccine development and other studies where immune activation is relevant. The same response can complicate vector use, particularly when researchers consider administering the vector more than once. Consequently, immune behavior is an important factor when planning applications and interpreting experimental outcomes.
Researchers modify the adenoviral genome by removing genes required for replication and inserting the DNA sequence of interest. They then use the resulting vector to deliver that transgene into mammalian cells and assess production of the encoded protein or its biological effect. This workflow connects vector construction with gene-expression or functional studies.
Gene expression studies can use the vector to produce a selected protein and examine its role in mammalian cells. Vaccine research can take advantage of strong immune stimulation, while cancer research and experimental gene therapy use the delivery system to investigate disease-related or therapeutic strategies. These applications reflect goals of studying expression, provoking immunity, or exploring treatment.