Recombinant Plasmids
Recombinant Plasmids - Related Videos
02:44
Generation of Recombinant Adeno-Associated Virus Through Plasmid Transfection
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2026
Source: Ding, J., et al. Preparation of rAAV9 to Overexpress or Knockdown Genes in Mouse Hearts. J. Vis. Exp. (2016)This video demonstrates the production of recombinant adeno-associated virus particles through plasmid transfection in mammalian cells. It highlights the coordinated expression of Rep, Cap, and helper proteins that drive viral genome replication and capsid assembly within the nucleus.
11:31
Generation of Recombinant Influenza Virus from Plasmid DNA
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Cited by 129 •
2010
Rescue of influenza A viruses from plasmid DNA is a basic and essential experimental technique that allows influenza researchers to generate recombinant viruses to study multiple aspects in the biology of influenza virus, and to be used as potential vectors or vaccines.
03:43
Plasmid-Mediated Generation of Recombinant Rotavirus in Cultured Cells
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2026
Source: Philip, A. A., et al. Simplified Reverse Genetics Method to Recover Recombinant Rotaviruses Expressing Reporter Proteins. J. Vis. Exp. (2020)This video demonstrates the recovery of recombinant rotavirus by transfecting plasmids encoding viral genome segments into hamster kidney cells, followed by amplification in monkey epithelial cells, enabling studies of rotavirus biology, replication, and vaccine development.
14:23
Recombineering Homologous Recombination Constructs in Drosophila
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Cited by 9 •
2013
Homologous recombination techniques greatly advance Drosophila genetics by enabling the creation of molecularly precise mutations. The recent adoption of recombineering allows one to manipulate large pieces of DNA and transform them into Drosophila6. The methods presented here combine these techniques to rapidly generate large homologous recombination vectors.
08:16
Plasmid Purification
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2023
Plasmid purification is a technique used to isolate and purify plasmid DNA from genomic DNA, proteins, ribosomes, and the bacterial cell wall. A plasmid is a small, circular, double-stranded DNA that is used as a carrier of specific DNA molecules. When introduced into a host organism via transformation, a plasmid will be replicated, creating numerous copies of the DNA fragment under study. In this video, a step-by-step generalized procedure is described for how to perform plasmid purification.
FAQs
The origin of replication enables the plasmid to be copied inside the host cell. This feature allows cells that receive the construct to maintain and replicate the selected DNA sequence as the cells propagate. Consequently, the origin is essential for retaining the recombinant plasmid during cloning, genetic analysis, and other experiments requiring continued plasmid presence.
Selectable markers help researchers identify host cells that received the plasmid after transformation. Cells carrying the construct can be distinguished from cells that did not acquire it, making the marker an important part of recovering the intended recombinant population. This selection step supports downstream gene cloning, expression studies, and analysis of the inserted sequence.
Restriction sites provide compatible locations for joining a target DNA fragment to a plasmid vector, while assembly methods offer another way to create the same recombinant construct. The selected approach determines how the insert is incorporated into the vector. Successful joining produces a plasmid carrying the sequence needed for later replication, analysis, or expression.
A typical workflow begins by inserting the selected DNA fragment into a plasmid vector using compatible restriction sites or an assembly method. The resulting construct is then introduced into bacteria through transformation. Researchers use the plasmid’s selectable marker to identify cells that received it, after which the recovered cells can support cloning, analysis, or expression studies.
Researchers use these constructs when they need to clone a gene, investigate its function, analyze genetic material, or study gene expression. They are also useful when a selected sequence must be maintained and copied inside a host cell. The same general strategy therefore supports both basic biology experiments and biotechnology-focused work involving engineered genes.
In biotechnology, recombinant plasmids provide a way to carry selected genes into host cells for protein production. This capability supports the manufacture of recombinant medicines, while related constructs help researchers investigate how genes function. Their value comes from connecting DNA insertion with plasmid replication, selection of transformed cells, and downstream study or production of the encoded product.