Guard Cells

Guard cells are specialized plant epidermal cells that surround stomata, adjustable pores responsible for balancing carbon dioxide uptake with water loss. They open when ion transport and osmotic changes increase guard-cell turgor, often in response to light, and close when water stress triggers abscisic acid signaling, ion efflux, and loss of turgor. By regulating stomatal aperture, guard cells control gas exchange, transpiration, and photosynthetic performance. Studying their signaling pathways and responses to environmental conditions helps explain plant adaptation, drought tolerance, and water-use efficiency, with applications in crop improvement and plant-environment research.

Guard Cells - Related Videos

Research

JoVE Journal - Biology

Stomata Tape-Peel: An Improved Method for Guard Cell Sample Preparation

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Cited by 29 •

2018

This protocol describes a method of preparing enriched stomatal guard cells that is useful for physiological and other biological studies.

Research

JoVE Journal - Environment
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Individual Culturing of Tigriopus Copepods and Quantitative Analysis of Their Mate-guarding Behavior

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Cited by 6 •

2018

Mate-guarding behavior plays an important role in reproduction of intertidal copepods of the genus Tigriopus. However, methods for studying this behavior have not been well described. Here we describe methods for: 1) individual culture of virgin Tigriopus animals, and 2) quantitative analysis of their mate-guarding behavior.

Live Imaging of Cell Motility and Actin Cytoskeleton of Individual Neurons and Neural Crest Cells in Zebrafish Embryos

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Cited by 18 •

2010

This protocol describes imaging of individual neurons or neural crest cells in living zebrafish embryos. This method is used to examine cellular behaviors and actin localization using fluorescence confocal time-lapse microscopy.

Research

JoVE Journal - Biology
Free Sample

Generation of Dispersed Presomitic Mesoderm Cell Cultures for Imaging of the Zebrafish Segmentation Clock in Single Cells

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Cited by 8 •

2014

Somitogenesis is a rhythmic developmental process that spatially patterns the body axis of vertebrate embryos. Previously, we developed transgenic zebrafish lines that use fluorescent reporters to observe the cyclic genes that drive this process. Here, we culture dispersed cells from these lines and image their oscillations over time in vitro.

Exploring the Potential of Mesenchymal Stem Cell Sheet on The Development of Hepatocellular Carcinoma In Vivo

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Cited by 10 •

2018

Here, we present a protocol to develop an in vivo cancer model using cell sheet technology. Such a model could be very useful for the evaluation of anticancer therapeutics.

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