Wound Infection
Wound Infection - Related Videos
07:16
Swine Model of Biofilm Infection and Invisible Wounds
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Cited by 12 •
2023
Chronic wounds that are resistant to antibiotics are a major threat to the healthcare system. Biofilm infections are stubborn and hostile and can cause deficient functional wound closure. We report a clinically relevant swine model of biofilm-infected full-thickness chronic wounds. This model is powerful for mechanistic studies as well as for testing interventions.
02:26
Establishment of Rice Plant Leaf Infection through Fungal Inoculation via Wounding
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2025
This video demonstrates the establishment of rice plant leaf infection through wounding inoculation with Magnaporthe grisea mycelium. The fungal spore-derived germ tube penetrates the epidermal layer, resulting in tissue infection and lesions on the leaves. This method aids pathotype screening and improves plant resistance understanding for molecular breeding.
02:40
Bioluminescence Imaging of Acinetobacter baumannii-Infected Burn Wounds in Mice
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2026
Source: Wang, Y., et al. In Vivo Investigation of Antimicrobial Blue Light Therapy for Multidrug-resistant Acinetobacter baumannii Burn Infections Using Bioluminescence Imaging. J. Vis. Exp. (2017)This video demonstrates the procedure for imaging burn wound infections in mice inoculated with Acinetobacter baumannii carrying a bioluminescent operon. It demonstrates how luciferase-catalyzed substrate oxidation produces light proportional to bacterial load, enabling real-time monitoring of...
02:13
Generating an Ex Vivo Ovine Wounded Skin Model Infected with a Bacterial Pathogen
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2025
The video demonstrates the generation of an ex vivo ovine skin model infected with Staphylococcus aureus. This entails treating a lamb's forehead skin biopsy section with antibiotics, establishing a wound bed, positioning it on a permeable membrane in a multiwell plate with antibiotic-free medium, and establishing bacterial infection in the skin wound.
02:24
Developing a Mouse Model of Chronic Pseudomonas aeruginosa Skin Wound Infection
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2026
Source: de Vries, C. R., et.al. A Delayed Inoculation Model of Chronic Pseudomonas aeruginosa Wound Infection. J. Vis. Exp. (2020)This video demonstrates the delayed inoculation of luminescent Pseudomonas aeruginosa into a mouse bearing two full-thickness dorsal skin wounds covered by a transparent film dressing. This facilitates biofilm formation and the establishment of chronic infection. Regular bioluminescence monitoring enables quantification of bacterial burden and tracking of infection...
FAQs
Biofilms form when microorganisms adhere to damaged tissue and organize within a protective microbial community. This growth pattern can help organisms persist at the wound site and may interfere with normal healing. Studying biofilm formation therefore helps researchers understand why some infections remain established, produce ongoing tissue damage, or respond differently to antimicrobial treatment.
Inflammation reflects the activity of immune cells and signaling molecules responding to microorganisms in damaged tissue. Although this response helps defend the wound, it can also contribute to redness, warmth, swelling, pain, and tissue damage when infection disrupts healing. Examining these reactions reveals important features of host defense and host–pathogen interactions.
Clinical features vary because the infecting organism and the type of wound influence how tissue and immune defenses respond. One wound may show prominent redness, warmth, swelling, or pus, while another may mainly exhibit delayed closure or tissue damage. This variation makes the organism, wound environment, and healing progress important subjects in biological investigation.
Biologists examine how microorganisms enter damaged tissue, adhere to it, multiply, and interact with host defenses. They also follow immune-cell activity, signaling molecules, biofilm formation, and changes in healing. Connecting these processes helps researchers explain disease progression and evaluate approaches aimed at limiting tissue damage or restoring normal wound closure.
Antimicrobial treatment research focuses on controlling the microorganisms associated with damaged tissue while considering the infection’s effects on healing. Biological studies can connect microbial behavior, including adherence and biofilm formation, with treatment-related outcomes. This work supports efforts to identify strategies that reduce persistent infection, tissue damage, and the possibility of broader illness.
Wound infection research informs wound care by linking microbial invasion, immune responses, delayed closure, and tissue damage. It also supports strategies intended to reduce complications such as tissue loss or systemic infection. In biology, this subject provides a practical context for studying host–pathogen interactions and for connecting cellular responses with health outcomes.