Liver Tissue Ablation
Liver Tissue Ablation - Related Videos
03:39
Targeted Liver Ablation: Inducing Hepatocyte Death with Metronidazole in Transgenic Zebrafish Larvae
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2023
The article describes targeted liver ablation, a method for inducing hepatocyte death with metronidazole in transgenic zebrafish larvae. The sample protocol describes the process for generating, manipulating and assessing targeted liver ablation in zebrafish.
08:14
Hepatocyte-specific Ablation in Zebrafish to Study Biliary-driven Liver Regeneration
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Cited by 18 •
2015
To help assess the molecular mechanisms underlying zebrafish biliary-driven liver regeneration, we established a liver injury model in which the nitroreductase-expressing hepatocytes are genetically ablated upon metronidazole treatment. In this protocol, we describe how to adeptly manipulate, monitor and analyze hepatocyte ablation and biliary-driven liver regeneration.
07:38
Real-time Monitoring of High Intensity Focused Ultrasound (HIFU) Ablation of In Vitro Canine Livers Using Harmonic Motion Imaging for Focused Ultrasound (HMIFU)
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Cited by 13 •
2015
This article describes real-time monitoring of HIFU ablation in canine liver with high frame rate ultrasound imaging using diverging and plane wave imaging. Harmonic Motion Imaging for Focused Ultrasound is used to image the decrease of acoustic radiation force induced displacement in the ablated region.
07:16
Thermal Ablation for the Treatment of Abdominal Tumors
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Cited by 19 •
2011
A thermal tumor ablation procedure is described. The entire procedure is detailed, including pretreatment planning and imaging studies, anesthesia, adjuvant techniques to facilitate a percutaneous approach, imaging guidance of the ablation device to the tumor, thermal treatment, post-treatment care and follow-up.
09:05
Imaging Metals in Brain Tissue by Laser Ablation - Inductively Coupled Plasma - Mass Spectrometry (LA-ICP-MS)
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Cited by 40 •
2017
Quantitatively mapping metals in tissue by laser ablation - inductively coupled plasma - mass spectrometry (LA-ICP-MS) is a sensitive analytical technique that can provide new insight into how metals participate in normal function and disease processes. Here, we describe a protocol for quantitatively imaging metals in thin sections of mouse neurological tissue.
FAQs
The three principal approaches produce injury through different biological mechanisms. Thermal energy denatures proteins, extreme cold disrupts cell membranes and cellular integrity, and chemical agents can produce coagulative necrosis, a form of tissue death caused by protein disruption. Comparing these mechanisms helps cancer researchers examine how the type of insult influences tumor destruction and responses in nearby liver tissue.
The treatment zone determines which tissue receives the damaging stimulus and which surrounding structures remain relatively preserved. This spatial distinction is important because research can separate direct tumor destruction from effects in adjacent liver tissue. It also supports investigation of local recurrence, tissue repair, and immune responses while maintaining attention to preservation of liver function.
Outcomes may differ because thermal energy, extreme cold, and chemical agents affect cells through distinct mechanisms. Their effects can therefore be evaluated in relation to membrane disruption, protein denaturation, or coagulative necrosis within the targeted region. In cancer research, this comparison helps connect the chosen approach with patterns of tumor destruction and subsequent tissue responses.
Post-treatment studies can examine more than whether the targeted tumor tissue was destroyed. They may assess local recurrence, the process of liver tissue repair, and immune responses that follow treatment. These endpoints provide a broader view of treatment effects, linking the immediate cellular injury with later changes in the treated region and its biological environment.
It may be considered for selected patients with primary liver tumors or tumors that have spread to the liver, particularly when surgery is unsuitable. Preservation of liver function is another important context for its use. These considerations place the method within a treatment strategy focused on localized disease while limiting the need for more extensive tissue removal.
The method provides a model for studying how localized treatment affects tumor behavior and the surrounding liver. Investigators can use it to explore recurrence at or near the treated region, repair of injured tissue, and immune responses after ablation. This makes the approach relevant to both treatment research and studies of interactions between cancer, tissue injury, and recovery.