Vessel Isolation
Vessel Isolation - Related Videos
04:20
Isolating Mouse Brain Vessels: A Protocol to Isolate Intact Vessels from Murine Brain Sample
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2025
This video demonstrates the isolation of intact vessels from a mouse's brain that constitute the blood-brain barrier (BBB) along with neurons and glial cells. The isolated vessels can be used as an in vitro model to study their role in maintaining brain homeostasis.
08:09
Vasodilation of Isolated Vessels and the Isolation of the Extracellular Matrix of Tight-skin Mice
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Cited by 1 •
2017
We describe the isolation of cardiac extracellular matrix from C57Bl/6J control mice, tight-skin mice, and tight-skin mice treated with the IRF5 inhibitory peptide. We also describe the vasodilation studies on the isolated vessels from C57Bl/6J, tight-skin mice and tight-skin mice treated with the IRF5 inhibitory peptide.
10:52
Isolation of Blood-vessel-derived Multipotent Precursors from Human Skeletal Muscle
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Cited by 22 •
2014
Blood vessels within human skeletal muscle harbor several multi-lineage precursor populations that are ideal for regenerative applications. This isolation method allows simultaneous purification of three multipotent precursor cell populations respectively from three structural layers of blood vessels: myogenic endothelial cells from intima, pericytes from media, and adventitial cells from adventitia.
13:10
Stability of Floating Vessels
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2023
Source: Alexander S Rattner and Kevin Rao Li Department of Mechanical and Nuclear Engineering, The Pennsylvania State University, University Park, PA The objective of this experiment is to demonstrate the phenomenon of stability of floating vessels - the ability to self-right when rolled over to the side by some external force. Careful design of hull shapes and internal mass distribution enables seagoing vessels to be stable with low drafts (submerged depth of hull), improving vessel...
07:32
Purification of Mouse Brain Vessels
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Cited by 90 •
2015
We describe a protocol allowing the purification of the mouse brain's vascular compartment. Isolated brain vessels include endothelial cells linked by tight junctions and surrounded by a continuous basal lamina, pericytes, vascular smooth muscle cells, as well as perivascular astroglial membranes.
FAQs
Mechanical and enzymatic dissociation serve complementary purposes: mechanical treatment breaks tissue apart, while enzymes help release vascular fragments or endothelial cells from surrounding material. The balance affects whether samples retain vessel architecture or yield separable cells. That distinction matters because structural studies require fragments, whereas molecular and cellular analyses may benefit from enriched endothelial populations.
Filtration, density-based separation, and marker-guided enrichment distinguish vascular material through different properties. Filtration separates fragments by physical passage, density-based methods separate material according to density, and marker-guided methods select cells according to molecular identifiers. The choice depends on whether a study prioritizes intact vascular structure, enriched endothelial cells, or molecularly defined populations.
Isolated vessels let investigators examine endothelial signaling, vascular permeability, and vessel interactions with cancer cells without surrounding tissue dominating the analysis. In tumors, these measurements help connect vascular behavior with angiogenesis and the tumor microenvironment. Comparing isolated material from normal and tumor-associated vasculature can reveal disease-associated differences in molecular profile or function.
A typical workflow begins with tissue dissociation, followed by separation of vascular fragments or endothelial cells from the resulting mixture. Filtration, density-based separation, or marker-guided enrichment can then concentrate the desired material. The recovered fraction is analyzed for structure, molecular profile, or function, depending on the research question. This staged approach links preparation with the intended vascular measurement.
Isolated vascular material can provide a basis for evaluating anti-angiogenic therapies by revealing changes in vessel-associated structure, signaling, permeability, or molecular profile. Because the preparation separates vascular components from surrounding tissue, treatment-related effects can be examined more directly. Comparisons among vascular samples help relate observed responses to cancer progression and therapeutic activity.
Vessel isolation supports biomarker research by supplying vascular material whose molecular profile can be examined independently of surrounding tissue. In cancer studies, this can help identify markers associated with tumor-associated vasculature and distinguish vascular changes linked to the tumor microenvironment. These profiles may clarify disease progression and support evaluation of vascular responses to anti-angiogenic treatment.