Real-time Metabolic Monitoring
Real-time Metabolic Monitoring - Related Videos
04:41
Real-Time Monitoring of Energy Metabolism in Human NK Cells Using an Extracellular Flux Analyzer
0 Views •
2025
This video demonstrates the method to analyze energy metabolism in activated human NK cells using an extracellular flux analyzer. Different inhibitors of the electron transport chain complexes impact ATP synthesis, which is measured through the oxygen consumption rate or OCR, providing insights into cellular metabolism and bioenergetics.
09:43
Using Real-Time Cell Metabolic Flux Analyzer to Monitor Osteoblast Bioenergetics
0 Views •
Cited by 5 •
2022
Real-time cell metabolic flux assay measures the oxygen consumption rate and extracellular acidification rate, which corresponds to mitochondrial and glycolytic adenosine triphosphate production, using pH and oxygen sensors. The manuscript explains a method to understand the energy status of osteoblasts and the characterization and interpretation of the cellular bioenergetic status.
04:47
Autonomously Bioluminescent Mammalian Cells for Continuous and Real-time Monitoring of Cytotoxicity
0 Views •
Cited by 7 •
2013
Mammalian cells expressing the bacterial bioluminescence gene cassette (lux) produce light autonomously. The resulting bioluminescent dynamics upon chemical exposure have been demonstrated to reflect the treatment effects on cellular growth and metabolism, making these cells an inexpensive, continuous, real-time toxicity screening tool that can easily be adapted for high-throughput automation.
01:27
Isothermal Microcalorimetry to Monitor Metabolic Activity in Bacteria
0 Views •
2025
Take serial dilutions of a bacterial culture and add aliquots into calorimetric vials containing growth medium.Load the sealed vials into the multi-well plate of an isothermal microcalorimeter to measure metabolic heat production.The device is held at a constant temperature to ensure the detected heat originates solely from bacterial metabolism.As bacteria metabolize nutrients and proliferate, they release heat that sensors detect in real time.In parallel, incubate identical vials in an...
06:52
An Assay for Real-Time Monitoring of Extracellular pH in Dental Biofilms
0 Views •
2025
This video demonstrates a real-time assay for monitoring extracellular pH in dental biofilms using a pH-sensitive ratiometric probe. When an in situ-grown bacterial biofilm is exposed to glucose, a reduction in extracellular pH near the bacterial biomass protonates the probe, leading to its increased concentration inside the bacteria. The disparity in emission wavelength between the protonated and unprotonated probe is utilized to calculate the pH across the extracellular matrix of the biofilm...
FAQs
These readouts capture different aspects of cellular metabolism. Oxygen consumption indicates changes associated with oxygen use, whereas extracellular acidification reflects shifts in the surrounding medium linked to cellular metabolic activity. Examining both signals, alongside nutrient utilization or metabolite production, can provide a broader metabolic profile than relying on a single measurement in cancer cells.
Time-resolved measurements show when metabolic changes occur and whether they persist, reverse, or develop during an experiment. A single endpoint can record the final state without showing the sequence of adaptations that produced it. Continuous observation therefore helps identify rapid responses to altered conditions, including changes that may precede visible differences in cell behavior.
Tumor cells can alter energy use and metabolic pathways as conditions change. Measurements collected during changes in nutrients, treatment exposure, or the tumor microenvironment can therefore reveal adaptive responses rather than a fixed metabolic state. This context helps distinguish an inherent metabolic phenotype from a response triggered by the surrounding conditions.
Researchers use sensors or analytical platforms to follow selected variables while living cells experience defined conditions. Depending on the study, the recorded signals may include oxygen consumption, extracellular acidification, nutrient utilization, or metabolite production. The resulting time courses can then support comparisons between cell populations or between untreated and experimentally altered conditions.
Drug-response studies can track metabolic changes as treatment proceeds instead of examining only a final endpoint. A response may appear as an altered oxygen-use pattern, extracellular acidification profile, nutrient utilization pattern, or level of metabolite production. Comparing these changes across conditions can help characterize treatment effects and identify metabolic adaptations associated with resistance.
Comparing healthy and malignant cells can reveal differences in how each population uses energy and responds to changing conditions. Dynamic measurements add information about the timing and direction of those differences, not just their final magnitude. Such comparisons help characterize cancer-associated metabolic phenotypes and can inform studies of progression and therapeutic development.