Line Spectra

Line spectra are patterns of discrete wavelengths emitted or absorbed by atoms and molecules, providing distinctive signatures of their composition and energy structure. They arise when electrons transition between quantized energy levels, releasing or absorbing photons whose wavelengths correspond to specific energy differences. In chemistry, emission and absorption line spectra help identify elements, determine the composition of unknown samples, and study electronic structure. Spectroscopic analysis based on these patterns supports flame tests, atomic absorption measurements, and the interpretation of light from distant astronomical objects, linking observable color and wavelength to the behavior of matter at the atomic scale.

Line Spectra - Related Videos

Education

JoVE Core - Chemistry

Emission Spectra

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2020

When solids, liquids, or condensed gases are heated sufficiently, they radiate some of the excess energy as light. Photons produced in this manner have a range of energies, and thereby produce a continuous spectrum in which an unbroken series of wavelengths is present. In contrast to continuous spectra, light can also occur as discrete or line spectra having very narrow linewidths interspersed throughout the spectral regions. Exciting a gas at low partial pressure using an electrical current,...

Research

JoVE Journal - Engineering

Measurement and Analysis of Atomic Hydrogen and Diatomic Molecular AlO, C2, CN, and TiO Spectra Following Laser-induced Optical Breakdown

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Cited by 9 •

2014

Time-resolved atomic and diatomic molecular species are measured using LIBS. The spectra are collected at various time delays following the generation of optical breakdown plasma with Nd:YAG laser radiation and are analyzed to infer electron density and temperature.

Research

JoVE Journal - Engineering
Free Sample

Method for Recording Broadband High Resolution Emission Spectra of Laboratory Lightning Arcs

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2019

Emission spectroscopy techniques have traditionally been used to analyze inherently random lightning arcs occurring in nature. In this paper, a method developed to obtain the emission spectroscopy from reproducible lightning arcs generated within a laboratory environment is described.

Flow Virometry to Analyze Antigenic Spectra of Virions and Extracellular Vesicles

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Cited by 9 •

2017

Viruses or extracellular vesicles were immunocaptured with 15 nm magnetic nanoparticles coupled to antibodies recognizing surface antigens. The captured virions or vesicles were labeled with fluorescent antibodies against other surface antigens. The resultant complexes were separated in high magnetic field and analyzed with conventional flow cytometers triggered on fluorescence.

Characterization of Biological Absorption Spectra Spanning the Visible to the Short-Wave Infrared

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Cited by 8 •

2025

Biological samples have distinct optical characteristics in the short-wave infrared (SWIR) compared to the visible (VIS) and near-infrared (NIR) wavelength ranges. However, recording pure SWIR absorption spectra is challenging and rarely conducted for biological molecules. This article presents a method to characterize the VIS-SWIR absorption spectra of biological absorbers.

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