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JoVE Journal
Immunology and Infection

This content is Open Access.

Flow Virometry to Analyze Antigenic Spectra of Virions and Extracellular Vesicles
 

Flow Virometry to Analyze Antigenic Spectra of Virions and Extracellular Vesicles

Article doi: 10.3791/55020
January 25th, 2017

Summary January 25th, 2017

Viruses or extracellular vesicles were immunocaptured with 15 nm magnetic nanoparticles coupled to antibodies recognizing surface antigens. The captured virions or vesicles were labeled with fluorescent antibodies against other surface antigens. The resultant complexes were separated in high magnetic field and analyzed with conventional flow cytometers triggered on fluorescence.

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