Scattering Length Density Maps

Scattering length density maps are spatial representations of how strongly different regions of a material scatter radiation, helping relate measured signals to chemical composition and structure. They are generated by assigning each component a scattering length density, determined by the types and concentrations of atoms, then reconstructing its distribution from diffraction, reflectometry, or small-angle scattering data through model fitting or inverse analysis. In chemistry, these maps reveal interfaces, layer thicknesses, molecular organization, and compositional variations in thin films, membranes, catalysts, and nanostructured materials. Comparing maps under different conditions can clarify adsorption, swelling, assembly, and structural changes that are difficult to resolve directly.

Scattering Length Density Maps - Related Videos

Research

JoVE Journal - Biology

Mapping Absolute DNA Density in Cell Nuclei using Single-molecule Localization Microscopy

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2025

The present protocol describes a method measuring absolute DNA densities within adherent cell nuclei using Voronoi tessellation of single-molecule localization microscopy data, known volume, genome size, and cell cycle stage.

Measurement of Scattering Nonlinearities from a Single Plasmonic Nanoparticle

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Cited by 2 •

2016

Saturable and reverse saturable scattering were discovered in isolated plasmonic particles and adopted as a novel non-bleaching contrast method in super-resolution microscopy. Here the experimental procedures of detecting and extracting nonlinear scattering are explained in detail, as well as how to enhance resolution with the aid of saturated excitation microscopy.

Research

JoVE Journal - Engineering
Free Sample

Measuring Spatially- and Directionally-varying Light Scattering from Biological Material

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Cited by 5 •

2013

We present a non-destructive method for sampling spatial variation in the direction of light scattered from structurally complex materials. By keeping the material intact, we preserve gross-scale scattering behavior, while concurrently capturing fine-scale directional contributions with high-resolution imaging. Results are visualized in software at biologically-relevant positions and scales.

Education

JoVE Science Education - Advanced Biology

Fate Mapping

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2023

Fate mapping is a technique used to understand how embryonic cells divide, differentiate, and migrate during development. In classic fate mapping experiments, cells in different areas of an embryo are labeled with a chemical dye and then tracked to determine which tissues or structures they form. Technological improvements now allow for individual cells to be marked and traced throughout embryonic development and adulthood. This video reviews the concepts behind fate mapping, and then details a...

Motor Maps

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2023

Source: Laboratories of Jonas T. Kaplan and Sarah I. Gimbel—University of Southern California One principle of brain organization is the topographic mapping of information. Especially in sensory and motor cortices, adjacent regions of the brain tend to represent information from adjacent parts of the body, resulting in maps of the body expressed on the surface of the brain. The primary sensory and motor maps in the brain surround a prominent sulcus known as the central sulcus. The cortex...

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