Skin Integrity
Skin Integrity - Related Videos
05:44
Skin Biopsy for Diagnosing Discoid Lupus Erythematosus
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Cited by 2 •
2025
Skin lesions are common and important features in many rheumatic diseases, and biopsy with pathological analysis plays a critical role in diagnosis. This article provides a detailed overview of the standardized skin biopsy procedure and, using discoid lupus erythematosus as an example, highlights the essential role of dermatopathology in diagnosing rheumatic diseases.
04:07
Generation of Organotypic Full Skin Reconstructs: A Procedure to Establish 3D Skin Model Using Human Skin Samples
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2023
This video describes the detailed protocol for generating in vitro 3D organotypic skin reconstructs to study melanoma progression. This skin model mimics both the architecture and multicellular complexity of organs in vivo.
06:42
Murine Skin Transplantation
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Cited by 36 •
2008
Allogeneic skin transplantation is a standard model to assay host T cell responses to MHC-disparate donor antigens. This video-article provides a visual tutorial of each step involved in performing a BALB/c-->C57BL/6 skin transplant.
09:30
Tissue Characterization after a New Disaggregation Method for Skin Micro-Grafts Generation
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Cited by 44 •
2016
The protocol describes a new method to disaggregate human tissues and to create autologous micro-grafts that, combined with collagen sponges, give rise to human bio-complexes ready to use in the treatment of skin lesions. Further, this system preserves cell viability of micro-grafts at different times after mechanical disaggregation.
05:36
Human Skin Keratinocyte Isolation: A Method to Culture Primary Keratinocytes from Skin Samples
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2023
This video describes the isolation of keratinocytes—the most prominent cells of human skin's epidermal layer, by employing a combination of enzymatic digestion and mechanical disruption methods. This method helps in the preferential growth of keratinocytes over other skin cell types using a selective growth medium.
FAQs
Epidermal renewal and keratinization continually maintain the outer protective layers, while intercellular lipids help preserve cohesion and barrier function. Immune activity adds biological defense against microbial invasion. Together, these processes help the skin tolerate everyday stress and remain functional, but disruption can increase susceptibility to breakdown.
Adequate perfusion and balanced moisture support repair after skin damage, whereas an unsuitable moisture environment can contribute to vulnerability when combined with other stresses. Clinically, this makes moisture management important alongside pressure redistribution, gentle cleansing, and moisturization, rather than treating hydration as an isolated concern.
These factors are clinically important contributors to skin breakdown, particularly when they affect pressure-prone areas or occur together. Immobility, friction, and shear place mechanical stress on tissue, while moisture and poor nutrition can undermine its ability to remain resilient and repair damage. Recognizing the combined risks supports earlier preventive action.
Assessment should include color, temperature, texture, turgor, lesions, and pressure-prone areas. These observations help clinicians identify early changes that may accompany breakdown associated with immobility, friction, shear, moisture, or poor nutrition. Repeated attention to these features supports timely recognition of problems before they progress to more extensive damage.
Core measures include repositioning, gentle cleansing, moisturization, and pressure redistribution. These actions address several preventable stresses while helping maintain a suitable surface environment. When damage is present or suspected, timely wound management should accompany preventive care, supporting repair and reducing the likelihood of complications in acute, long-term, and community settings.
The same principles apply across care settings, but the circumstances creating risk may differ. Clinical teams can combine structured observation with repositioning, pressure redistribution, gentle cleansing, moisturization, and timely wound management. This approach helps identify early breakdown, reduce complications, and support healing for patients whose mobility, moisture balance, nutrition, or tissue condition is compromised.