Tissue Characterization after a New Disaggregation Method for Skin Micro-Grafts Generation

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Medicine

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JoVE Journal Medicine

Tissue Characterization after a New Disaggregation Method for Skin Micro-Grafts Generation

DOI:

10.3791/53579-v

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09:30 min

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March 04, 2016

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Valeria Purpura, Elena Bondioli, Antonio Graziano, Letizia Trovato, Davide Melandri, Martina Ghetti, Andrea Marchesini, Maria Gabriella Cusella De Angelis⁵, Laura Benedetti⁵, Gabriele Ceccarelli⁵, Michele Riccio

¹Burns Centre and Emilia Romagna Regional Skin Bank, ²Human Brain Wave srl, ³Plastic and Reconstructive Surgery,AOU “Ospedali Riuniti”, ⁴Department of Public Health, Experimental Medicine, Anatomy and Forensic,University of Pavia, ⁵C.H.T Centre for Health Technologies,University of Pavia

Chapters

00:05Title
00:45Bio-complex Building for Clinical Application
02:59Collection Disaggregation and In Vitro Analysis of Tissues
06:40Results: Skin Disaggregation and Tissue Characterization
08:18Conclusion

Summary

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The protocol describes a new method to disaggregate human tissues and to create autologous micro-grafts that, combined with collagen sponges, give rise to human bio-complexes ready to use in the treatment of skin lesions. Further, this system preserves cell viability of micro-grafts at different times after mechanical disaggregation.

Tissue Characterization after a New Disaggregation Method for Skin Micro-Grafts Generation

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