Forebrain Electroporation
Forebrain Electroporation - Related Videos
06:00
Forebrain Electrophysiological Recording in Larval Zebrafish
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Cited by 55 •
2013
A simple method to record extracellular field potentials in the larval zebrafish forebrain is described. The method provides a robust in vivo read-out of seizure-like activity. This technique can be used with genetically modified zebrafish larvae carrying epilepsy-related genes or seizures evoked by administration of convulsant drugs.
07:49
Time-lapse Live Imaging of Clonally Related Neural Progenitor Cells in the Developing Zebrafish Forebrain
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Cited by 11 •
2011
The present video demonstrates a method which takes advantage of the combination of electroporation and confocal microscopy to perform live imaging on individual neural progenitor cells in the developing zebrafish forebrain. In vivo analysis of the development of forebrain neural progenitor cells at a clonal level can be achieved in this way.
02:05
In Vivo Electroporation: A Site-Specific Method to Transfect Zebrafish
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2023
This video describes in vivo electroporation, which is a technique for DNA delivery into ependymoglial cells in adult zebrafish.
11:57
Electroporation of Mycobacteria
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Cited by 55 •
2008
Mycobacterial pathogenic strategies remain poorly understood. The slow growth rate of most species, the impenetrable nature of the cell-wall, and the hazards of working with pathogens make mycobacteria difficult to study and are largely responsible for our poor understanding of these organisms. In this video we will demonstrate the technique of electroporation, which involves subjecting cells to a brief high electrical impulse to allow the entry of DNA. It is the most widely used method for...
07:21
Two-Photon Calcium Imaging of Forebrain Activity in Behaving Adult Zebrafish
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Cited by 1 •
2023
Here, we present a protocol to perform two-photon calcium imaging in the dorsal forebrain of adult zebrafish.
FAQs
Polarity and electrode placement help determine which forebrain region receives the introduced nucleic acids. The electrical field is therefore not merely a delivery aid; its orientation contributes to spatial targeting within embryonic brain tissue. This localization allows investigators to associate a genetic manipulation with developmental changes in particular forebrain regions rather than affecting the entire brain uniformly.
Brief electrical pulses transiently increase the permeability of cell membranes, creating a limited opportunity for nearby DNA or other genetic material to enter developing cells. Because the permeability change is temporary, pulse delivery must coincide with the placement of the genetic material near the embryonic tissue. This mechanism supports rapid manipulation without requiring permanent alteration of every surrounding cell.
The resulting manipulation can be linked to several stages of neural development, including cell proliferation, migration, differentiation, axon formation, and circuit development. Examining these outcomes helps reveal whether a gene acts early by influencing cell production or positioning, or later by affecting neuronal specialization, axonal growth, or circuit organization in the developing forebrain.
A typical workflow places DNA or another nucleic acid near embryonic forebrain tissue, positions electrodes so the intended region lies within the relevant electrical field, and applies brief electrical pulses. The material, tissue location, pulse delivery, and electrode orientation work together to determine which cells are exposed and how spatially restricted the manipulation becomes.
Researchers use this approach when they need to manipulate gene expression in vivo while examining neural development in its tissue context. Its rapid delivery and spatial targeting make it useful for testing functional roles of genes during forebrain formation, especially when the goal is to connect localized genetic changes with specific cellular or structural developmental outcomes.
The technique can be used to alter gene expression in developing forebrain cells and then examine consequences for proliferation, migration, differentiation, axon formation, or circuit development. Applying these readouts to disease-associated pathways helps investigators study how altered gene regulation may influence neural development in vivo, while the targeted design helps relate effects to particular forebrain regions.