Zebrafish Keratocytes
Zebrafish Keratocytes - Related Videos
09:35
Zebrafish Keratocyte Explants to Study Collective Cell Migration and Reepithelialization in Cutaneous Wound Healing
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Cited by 11 •
2015
Zebrafish keratocytes migrate in cell sheets from explants and provide an in vitro model for the study of the mechanisms of collective cell migration in the context of epithelial wound healing. These protocols detail an effective way to establish primary explant cultures for use in collective cell migration assays.
06:55
In vivo Electroporation of Morpholinos into the Adult Zebrafish Retina
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Cited by 42 •
2011
A method to conditionally knockdown a target protein’s expression in the adult zebrafish retina is described, which involves intravitreally injecting antisense morpholinos and electroporating them into the retina. The resulting protein is knocked down for several days, which allows testing the protein’s role in the regenerating or intact retina.
07:58
Nephrotoxin Microinjection in Zebrafish to Model Acute Kidney Injury
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Cited by 8 •
2016
Renal injuries incurred from nephrotoxins, which include drugs ranging from antibiotics to chemotherapeutics, can result in complex disorders whose pathogenesis remains incompletely understood. This protocol demonstrates how zebrafish can be used for disease modeling of these conditions, which can be applied to the identification of renoprotective measures.
08:07
Zebrafish Reproduction and Development
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2023
The zebrafish (Danio rerio) has become a popular model for studying genetics and developmental biology. The transparency of these animals at early developmental stages permits the direct visualization of tissue morphogenesis at the cellular level. Furthermore, zebrafish are amenable to genetic manipulation, allowing researchers to determine the effect of gene expression on the development of a vertebrate with a high degree of genetic similarity to humans. This video provides a brief overview of...
05:40
Tol2 Transposon-Mediated Zebrafish Transgenesis: A Procedure to Generate Transgenic Zebrafish Following the Coinjection of Tol2 System Into Fertilized Zebrafish Embryos
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2025
In this video, we demonstrate a procedure to generate a stable zebrafish transgenic line constitutively expressing a fluorescent voltage indicator protein following the co-injection of the Tol2 transposon system comprising the transposase mRNA and Tol2 transposon construct encoding the fluorescent voltage indicator.
FAQs
Following corneal damage, local signals initiate coordinated changes rather than a single cellular response. Zebrafish keratocytes remodel their cytoskeleton, adjust adhesion turnover, and migrate in a directed manner toward the affected region. These linked processes let researchers examine how signals are converted into cell movement and how individual behaviors contribute to organized corneal repair.
Cytoskeletal remodeling changes the internal structure that supports cell shape and movement, while adhesion turnover allows attachments to the surrounding tissue to be repeatedly adjusted. Together, these mechanisms help keratocytes move directionally after injury instead of remaining fixed in place. Studying both processes clarifies how cellular mechanics support coordinated tissue-level wound healing.
During repair, keratocytes can alter their matrix-producing activity and cellular phenotype. This flexibility means the same stromal cell population may contribute differently as the tissue responds to damage and restores organization. Examining these changes helps developmental biologists relate cell-state regulation to extracellular matrix organization, repair progression, and the maintenance of corneal structure.
Their value comes from connecting visible cell behavior with broader tissue processes. Because zebrafish keratocytes are accessible to imaging and occur in a system with regenerative capacity, researchers can examine how cellular activities relate to corneal formation, ongoing maintenance, and repair. This creates a developmental biology model that links mechanisms at the cellular and tissue scales.
Imaging can be used to follow keratocyte behavior within living tissue, including responses associated with injury, directed migration, and changes in cellular organization. Observations can then be related to cytoskeletal remodeling, adhesion turnover, and matrix-producing activity. The resulting view helps researchers connect dynamic cell behavior with the progression of corneal wound healing and regeneration.
This model is suited to questions about how corneal stromal cells respond to damage, reorganize their surroundings, and participate in tissue recovery. It also supports investigation of extracellular matrix organization, cell migration, and regeneration. In developmental biology, the same system can help relate these repair processes to the formation and maintenance of the cornea.