Capillary Breakup Elongational Rheometer

Capillary Breakup Elongational Rheometer (CaBER) is an instrument that measures how a liquid resists stretching, providing information that conventional shear rheometers may not capture. In a typical test, a small sample forms a bridge between two plates that rapidly separate; surface tension drives capillary thinning while viscosity and elastic stresses control the filament’s breakup. Images of the evolving diameter allow researchers to determine breakup time, extensional relaxation behavior, and related rheological properties. In engineering, CaBER supports the formulation and processing of polymer solutions, coatings, adhesives, inks, and other complex fluids, helping predict filament formation, spray behavior, and material performance in manufacturing and product design.

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Education

JoVE Core - Molecular Biology

Transcription Elongation Factors

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2020

Transcription elongation is a dynamic process that alters depending upon the sequence heterogeneity of the DNA being transcribed. Hence, it is not surprising that the elongation complex's composition also varies along the way while transcribing a gene. The transcription elongation is regulated via pausing of RNA polymerase on several occasions during transcription. In bacteria, these halts are necessary because the transcription of DNA into mRNA is coupled to the translation of that mRNA into a...

Transcription Elongation Factors

0 Views •

2023

Transcription elongation is a dynamic process that alters depending upon the sequence heterogeneity of the DNA being transcribed. Hence, it is not surprising that the elongation complex's composition also varies along the way while transcribing a gene. The transcription elongation is regulated via pausing of RNA polymerase on several occasions during transcription. In bacteria, these halts are necessary because the transcription of DNA into mRNA is coupled to the translation of that mRNA into a...

Research

JoVE Journal - Medicine

Intranasal Delivery of mRNA Polyplexes via Rayleigh Breakup Aerosols: An In Vitro Method for Nasal Deposition and Functional Testing

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2026

This protocol presents Rayleigh breakup as a mild process for aerosolizing non-viral mRNA vectors. Regional deposition for aerosolized mRNA vectors is evaluated by the Alberta Idealized Nasal Inlet (AINI) model. Green fluorescence protein (GFP) expression in human lung cells represents maintenance of physicochemical and biological functions of post-aerosolized mRNA vectors.

Novel Metrics to Characterize Embryonic Elongation of the Nematode Caenorhabditis elegans

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Cited by 3 •

2016

Here we detail protocols specifically designed to monitor morphogenic defects that occur during early and late phases of embryonic elongation of the nematode Caenorhabditis elegans. Ultimately, these protocols are designed to identify genes that regulate these phases and to characterize their differential requirements along the antero-posterior axis of the embryo.

Capillary Electrophoresis (CE)

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2023

Source: Laboratory of Dr. B. Jill Venton - University of Virginia Capillary electrophoresis (CE) is a separation technique that separates molecules in an electric field according to size and charge. CE is performed in a small glass tube called a capillary that is filled with an electrolyte solution. Analytes are separated due to differences in electrophoretic mobility, which varies with charge, solvent viscosity, and size. Traditional electrophoresis in gels is limited in the amount of voltage...

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