Dewaxing Xylene
Dewaxing Xylene - Related Videos
08:55
Histological Staining of Neural Tissue
0 Views •
2023
In order to examine the cellular, structural and molecular layout of tissues and organs, researchers use a method known as histological staining. In this technique, a tissue of interest is preserved using chemical fixatives and sectioned, or cut into very thin slices. A variety of staining techniques are then applied to provide contrast to the visually uniform sections. In the study of neuroanatomy, histological techniques are frequently applied to visualize and study nervous system tissue.
21:38
Immunohistochemistry: Paraffin Sections Using the Vectastain ABC Kit from Vector Labs
0 Views •
Cited by 36 •
2007
Immunohistochemistry (IHC) is a valuable technique utilized to localize/visualize protein expression in a mounted tissue section using specific ...
05:23
Use of Fluorescent Immuno-Chemistry for the detection of Edwardsiella ictaluri in channel catfish (I. punctatus) samples
0 Views •
Cited by 2 •
2011
Here we describe a procedure allowing the labeling of Edwardsiella ictaluri in situ in histological sections from channel catfish Ictalurus punctatus using indirect immunohistochemistry with monoclonal antibodies Ed9 as a primary, and fluorescent FitC labeled antibodies as a secondary. This allowed for the detection of the bacterium using fluorescent microscopy.
02:43
Deparaffinization and Rehydration of Tissue Sections: A Two-step Procedure to Remove Paraffin Wax from FFPE Tissue Slides Followed by Tissue Rehydration
0 Views •
2023
In this video, we demonstrate the processing of FFPE or formalin-fixed paraffin-embedded sections obtained from gastrointestinal tumor tissue. The deparaffinization and rehydration steps allow the processing of the tissue specimen for subsequent analytical applications.
03:18
Periodic Acid Schiff or PAS Staining: A Method to Assess the Structural Deformities of Glomerulus
0 Views •
2023
In this video, we describe the protocol for performing Periodic Acid Schiff (PAS) stain to assess the structural anomalies in the kidney glomerulus. The PAS staining method enables appropriate evaluation of the glomerulus pathophysiology.
FAQs
Xylene removes paraffin because its nonpolar character is compatible with the hydrophobic wax. Contact with successive xylene baths dissolves and strips the embedding medium from the tissue section, rather than simply rinsing it away. Removing that barrier helps later staining reagents and antibodies reach tissue structures for microscopic evaluation.
Graded alcohol and water restore hydration after the nonpolar solvent has removed paraffin. This transition prepares the section for subsequent staining and microscopic analysis, because the tissue must be brought into an aqueous-compatible state. The sequence therefore links wax removal to effective dye or antibody access rather than treating dewaxing as an isolated step.
Xylene's volatility can release solvent vapors that contribute to laboratory air pollution, while used solvent becomes hazardous waste. These impacts make the handling stage environmentally significant even when the histological result is satisfactory. Controls such as ventilation, closed handling, collection, recycling, and responsible disposal reduce uncontrolled release and support safer waste management.
A supported workflow moves the embedded section through sequential xylene baths, followed by graded alcohol and then water. The first stage removes paraffin; the later stages rehydrate the tissue. Keeping these stages in sequence prepares the section for staining or antibody-based analysis while supporting the intended preparation pathway.
Ventilation limits the buildup of volatile solvent in laboratory air, while closed handling reduces opportunities for vapor release during transfers and use. Collection keeps spent xylene out of uncontrolled waste streams, and recycling can reduce disposal demand. Responsible disposal remains necessary for material that cannot be recovered.
It is relevant whenever paraffin-embedded tissue must undergo staining and microscopic examination, including histopathology, biomedical research, and immunohistochemistry. In these settings, removing the embedding medium improves access for dyes or antibodies. Laboratories must balance that analytical benefit with solvent-management practices that address xylene vapors and hazardous waste.