Race Technique
Race Technique - Related Videos
02:35
RACE - Rapid Amplification of cDNA Ends
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2021
Rapid Amplification of cDNA Ends, or RACE, is one of the most effective methods to obtain a full-length cDNA from an mRNA sequence between a known internal region to the unknown sequence at the 5’ or 3’ end. The unknown region is cloned in the cDNA by a gene-specific primer that binds the known end, and a hybrid primer that attaches a predefined anchor sequence to the unknown end of the cDNA. The sequence in between is amplified by PCR with an anchor primer and a gene-specific primer. Since the...
11:48
A Contrast of Three Inoculation Techniques used to Determine the Race of Unknown Fusarium oxysporum f.sp. niveum Isolates
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Cited by 1 •
2021
Managing Fusarium wilt of watermelon requires knowledge of the pathogen races present. Here, we describe the root-dip, infested kernel seeding, and modified tray-dip inoculation methods to demonstrate their efficacy in race-typing of the pathogenic fungus Fusarium oxysporum f. sp niveum (Fon).
08:13
Using the Race Model Inequality to Quantify Behavioral Multisensory Integration Effects
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Cited by 20 •
2019
The current study aims to provide a step-by-step tutorial for calculating the magnitude of multisensory integration effects in an effort to facilitate the production of translational research studies across diverse clinical populations.
06:47
Combined Recombinase Polymerase Amplification CRISPR/Cas12a Assay for Detecting Fusarium oxysporum f. sp. cubense Tropical Race 4
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2025
In this protocol, a combined recombinase polymerase amplification CRISPR/Cas12a assay is illustrated for the detection of the invasive plant pathogen, Fusarium oxysporum f. sp. cubense tropical race 4.
04:14
Lab Techniques - Concepts
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2020
Measurement Accuracy The ability to repeat an experiment and get the same results, or reproducibility, is essential in scientific research. However, it’s impossible to repeat an experiment if you don’t know how you did it. Thus, scientists keep detailed records of their experiments in lab notebooks. These records include important information like the amount of each material used and descriptions of each step of the procedure. Equipment, like balances and volumetric or graduated glassware, is...
FAQs
The gene-specific primer anchors amplification to a known region of the transcript, while the adapter-specific primer recognizes the added or identified adapter sequence. Their pairing allows PCR to copy the previously unknown transcript end between these two reference points. This primer arrangement links known gene sequence information with the uncharacterized 5′ or 3′ boundary.
Adapter recognition supplies a defined priming site where the transcript end itself provides no known sequence for primer design. After reverse transcription creates cDNA, an adapter can be added or recognized, enabling an adapter-specific primer to participate in PCR. This converts an unknown boundary into a sequence-defined target that can be amplified and analyzed.
Different amplified transcript ends can indicate that RNA molecules from the same gene do not share identical boundaries. Comparing the recovered 5′ or 3′ ends therefore helps distinguish alternative transcript forms and shows how transcription produces distinct RNA molecules. These differences provide information for refining gene models and interpreting transcript organization.
A typical workflow begins with RNA, followed by reverse transcription to generate complementary DNA. An adapter sequence is then added or recognized, and PCR is performed with an adapter-specific primer paired with a gene-specific primer. The resulting products can be examined to determine the previously unknown transcript end and support reconstruction of a fuller cDNA sequence.
The choice depends on which transcript boundary remains unknown. A 5′ approach investigates the beginning of the messenger RNA, whereas a 3′ approach investigates its opposite end. Selecting the direction that addresses the missing boundary helps researchers map transcript limits efficiently and obtain sequence information needed for fuller gene characterization.
RACE results can support full-length cDNA cloning, transcript boundary mapping, and detection of alternative transcript forms. In genetics, these findings improve gene annotation by showing more complete transcript structures. They also clarify how transcription generates distinct RNA molecules, making the technique useful when existing gene sequence information does not define an entire messenger RNA.