Cd4 Cd8 Double Positive
Cd4 Cd8 Double Positive - Related Videos
10:57
Cell Cycle Analysis: Assessing CD4 and CD8 T Cell Proliferation After Stimulation Using CFSE Staining and Flow Cytometry
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2023
Source: Perchet Thibaut1,2,3, Meunier Sylvain1,2,3, Sophie Novault4, Rachel Golub1,2,3 1 Unit for Lymphpoiesis, Department of Immunology, Pasteur Institute, Paris, France 2 INSERM U1223, Paris, France 3 Université Paris Diderot, Sorbonne Paris Cité, Cellule Pasteur, Paris, France 4 Flow Cytometry Platfrom, Cytometry and Biomarkers UtechS, Center for Translational Science, Pasteur Institute, Paris, France The cell cycle is a universal process of life. During the cell cycle, a cell undergoes...
06:08
Generating Single Positive CD8 T Cells from Induced Pluripotent Stem Cells
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2025
This video demonstrates an assay to generate tumor antigen-specific induced pluripotent stem cell (iPSC)-derived CD8αβ+ T Cells. Tumor antigen-specific iPSCs are grown on a stromal cell monolayer and differentiated into hematopoietic progenitor cells (HPCs). The HPCs are differentiated into CD4+ CD8+ double-positive T cells using a differentiation medium. These cells are then stimulated to generate single-positive T cells expressing CD8.
06:20
An Assay for Cell Cycle Analysis of Antigen-Specific CD8+ T Cells
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2025
This video demonstrates an assay to analyze antigen-specific CD8+ T cells in different cell cycle stages. Fluorescently-labeled viable cells are selected using flow cytometry, and the cells in the different cell cycle stages are identified by their DNA content using antibodies against cell proliferation-associated nuclear protein and a nucleic acid stain.
06:39
Selective Isolation of CD4+ Vδ1+ γδ T Cells from Human Peripheral Blood Mononuclear Cells
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2025
This video demonstrates a protocol for the magnetic labeling-based isolation of a low-concentration population of CD4+Vδ1+γδ T cells from human peripheral blood mononuclear cells (PBMCs). Using a combination of FITC-conjugated anti-Vδ1 antibodies, magnetic anti-FITC microbeads, and anti-CD4 antibody-coated superparamagnetic beads, the CD4+Vδ1+ T-cells can be successfully purified from the PBMC population.
05:41
Nanoparticle-Based Enrichment and Expansion of Antigen-Specific CD8+ T Cells
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2025
This video describes a protocol for isolating rare-antigen-specific CD8+ T cells using magnetic nanoparticle-based artificial antigen-presenting cells (aAPCs) functionalized with antigenic peptide-loaded MHC-Igs and anti-CD28 antibodies. Interaction of the aAPCs with CD8+ T cells specifically expressing the antigenic peptide-targeting T cell receptors, followed by their expansion in vitro, results in the enrichment and expansion of this CD8+ T cell population.
FAQs
T-cell receptor recognition of self-peptide–MHC complexes determines whether a developing thymocyte receives a survival signal and proceeds toward maturation. Productive recognition supports continued development, while insufficient recognition fails to provide the signals needed for survival. This checkpoint connects receptor rearrangement with the later emergence of functional CD4 or CD8 single-positive T cells.
Weak recognition can cause death by neglect because the thymocyte does not receive adequate support for survival. Excessively strong recognition instead promotes negative selection, eliminating cells with potentially harmful self-reactivity. Distinguishing these outcomes is essential because thymic selection must preserve useful T-cell receptors while limiting the development of cells that could undermine central tolerance.
Lineage commitment follows productive T-cell receptor recognition during the double-positive stage. The selection process directs surviving cells toward either the CD4 or CD8 single-positive state, linking receptor testing to coreceptor-defined maturation. Examining this transition helps researchers study how the thymus establishes the T-cell populations that later participate in immune responses.
Researchers examine these cells with flow cytometry, tissue analysis, and genetic models. Flow cytometry can characterize cell populations, tissue analysis places developmental changes within the thymus, and genetic models help test how altered genes affect selection or maturation. Using these approaches together connects cellular measurements with tissue-level development and the mechanisms governing central tolerance.
Genetic models allow investigators to evaluate how specific developmental or selection mechanisms influence thymocytes. By examining changes in the progression from the double-positive stage to CD4 or CD8 single-positive cells, researchers can relate gene function to receptor selection, central tolerance, and immune development. These models are especially useful when normal thymic maturation is disrupted.
These thymocytes provide a developmental framework for interpreting altered T-cell responses during infection. Changes affecting thymic development or selection may also relate to thymic dysfunction, immunodeficiency, or autoimmunity. Studying the double-positive stage therefore helps connect early receptor testing and tolerance establishment with the quality and composition of T-cell populations available for later immune responses.