Mouse Lung Imaging
Mouse Lung Imaging - Related Videos
11:26
Real-time X-ray Imaging of Lung Fluid Volumes in Neonatal Mouse Lung
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Cited by 1 •
2016
We present a protocol to assess the rate of alveolar fluid clearance or pulmonary edema in neonatal mouse lung using X-ray imaging technology.
09:00
Vascular Casting of Adult and Early Postnatal Mouse Lungs for Micro-CT Imaging
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Cited by 10 •
2020
The aim of this technique is ex vivo visualization of pulmonary arterial networks of early postnatal and adult mice through lung inflation and injection of a radio-opaque polymer-based compound via the pulmonary artery. Potential applications for casted tissues are also discussed.
07:06
A Simple Method of Mouse Lung Intubation
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Cited by 50 •
2013
This paper describes a striaghforward and efficient method of intubating mice for pulmonary function measurements or pulmonary instillation, that allows the mice to recover and be studied at later times. The procedure involves an inexpensive fiberoptic light source that directly illuminates the trachea.
09:08
Multiple-mouse Neuroanatomical Magnetic Resonance Imaging
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Cited by 59 •
2011
Magnetic resonance imaging (MRI) has become an increasingly popular tool for examining the phenotype of genetically altered mice. This article illustrates the methods necessary to achieve high-throughput phenotyping of genetically altered mice using multiple-mouse MRI.
05:55
Whole Lung Agarose Inflation: A Method of Preparing Lungs for Ex vivo Live Imaging
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2023
This video describes a method of preparing lungs for ex vivo live imaging to study interactions between cancer cells and stromal cells, using agarose for inflation. In the sample protocol, agarose inflation procedure is described before observing live cellular interactions.
FAQs
Respiration continually moves the lung, which can obscure cellular and tissue-level observations. Motion-control strategies help stabilize the imaging field so researchers can follow leukocyte recruitment, pathogen interactions, and tissue changes more clearly over time. This stabilization is especially important when interpreting dynamic immune responses during inflammation, infection, or pulmonary injury.
Fluorescent labels make selected cells, pathogens, or tissue features visible, while optical access through a thoracic window or an exposed lung provides a path for microscopy. Used together, these components connect a labeled biological target with its location and behavior in lung tissue, supporting analysis of host-pathogen interactions and immune-cell movement.
Living preparations allow researchers to examine cellular behavior and tissue changes as they occur, including immune-cell recruitment during disease processes. Fixed samples provide a way to inspect lung structure and other preserved features after collection. Comparing these approaches can relate dynamic events to the structural context of inflammation, infection, or injury.
A typical study selects the biological feature to visualize, applies suitable fluorescent labels, establishes optical access with a thoracic window or exposed lung, and uses motion-control strategies to account for respiration. Microscopy then records structural features or cellular behavior, allowing observations to be related to infection, immune responses, inflammation, or pulmonary injury.
Repeated observations can show when and where leukocytes are recruited, how pathogens interact with host tissue, and how lung structures change during disease. These cellular dynamics add information that static observations may not provide. Researchers can then connect immune-cell behavior and tissue changes with infection outcomes, inflammatory responses, or pulmonary injury.
In immunology and infection studies, the approach helps examine relationships among immune cells, pathogens, lung tissue, and disease progression. Researchers can use these observations in mechanistic studies and when evaluating vaccines, antimicrobial treatments, or other interventions. The resulting visual evidence supports assessment of how an intervention affects immune responses and infection-related tissue changes.