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Vascular Casting of Adult and Early Postnatal Mouse Lungs for Micro-CT Imaging
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1Translational Vascular Medicine Branch, National Heart Lung and Blood Institute,National Institutes of Health, 2Mouse Imaging Facility, National Institute of Neurological Disorders and Stroke,National Institutes of Health, 3Division of Pediatric Surgery, Department of Surgery,University of North Carolina at Chapel Hill
Chapters
- 00:04Introduction
- 00:52Pulmonary Artery (PA) Catheterization and Blood Perfusion
- 03:18Tracheostomy and Lung Inflation
- 04:59Casting the Vasculature
- 06:44Results: Representative Successful Vascular Casting and Common Errors during Polymer Compound Infusion
- 08:24Conclusion
The aim of this technique is ex vivo visualization of pulmonary arterial networks of early postnatal and adult mice through lung inflation and injection of a radio-opaque polymer-based compound via the pulmonary artery. Potential applications for casted tissues are also discussed.
