Portable Pcr Unit
Portable Pcr Unit - Related Videos
08:46
Rapid Diagnosis of Avian Influenza Virus in Wild Birds: Use of a Portable rRT-PCR and Freeze-dried Reagents in the Field
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Cited by 20 •
2011
This study describes diagnosis of avian influenza in wild birds using a portable rRT-PCR system. The method takes advantage of freeze-dried reagents to screen wild birds in a non-laboratory setting, typical of an outbreak scenario. Use of molecular tools provides accurate and sensitive alternatives for rapid diagnosis.
14:15
On-Site Molecular Detection of Soil-Borne Phytopathogens Using a Portable Real-Time PCR System
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Cited by 25 •
2018
Rapid and accurate detection of plant pathogens on-site, especially soil-borne pathogens, is crucial to prevent further inoculum production and proliferation of plant diseases in the field. The method developed here using a portable real-time PCR detection system enables on-site diagnosis under field conditions.
08:51
Genotyping of Staphylococcus aureus by Ribosomal Spacer PCR (RS-PCR)
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Cited by 9 •
2016
Here, ribosomal spacer PCR (RS-PCR) is used together with a miniaturized electrophoresis system as a fast and high resolution method for genotyping S. aureus at moderate costs allowing a high throughput.
09:34
Implementation of Portable Emissions Measurement Systems (PEMS) for the Real-driving Emissions (RDE) Regulation in Europe
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Cited by 43 •
2016
The European Commission has developed a Real-Driving Emissions (RDE) test procedure to verify pollutant emissions during real-world vehicle operation using the Portable Emissions Measurement Systems (PEMS). This paper presents the experimental procedures required by the newly-adopted RDE test.
03:38
Measurement: Standard Units
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2020
Every measurement provides three kinds of information: the size or magnitude of the measurement (a number), a standard of comparison for the measurement (a unit), and an indication of the uncertainty of the measurement. While the number and unit are explicitly represented when a quantity is written, the uncertainty is an aspect of the errors in the measurement results. The number in the measurement can be represented in different ways, including decimal form and scientific notation, which is...
FAQs
Amplification depends on repeating three linked temperature-controlled events: DNA strands denature, primers bind their complementary target sequences, and new strands are extended. Repeating these cycles increases the amount of pathogen-specific nucleic acid available for detection. In a portable instrument, this thermal program is carried out in a compact format, allowing analysis near the sampling location rather than requiring immediate transfer to a centralized laboratory.
Primers determine which nucleic-acid sequence the assay attempts to amplify. During the binding stage, they identify the target sequence, so the resulting signal is linked to the presence of pathogen-specific material in the sample. This component gives the test its biological focus, allowing the same underlying amplification process to support different infection-related targets in clinical or environmental samples.
Fluorescence provides a way to monitor amplification as it occurs rather than relying only on an endpoint observation. As target material is amplified through successive cycles, the instrument can track the associated fluorescent readout. This monitoring is especially useful for near-site testing because it connects the molecular reaction to a timely result without requiring the sample to be analyzed on conventional laboratory equipment.
Compared with a workflow dependent on centralized equipment, portability changes where molecular testing can take place. The compact unit can be used near the site of collection, and its reduced dependence on laboratory infrastructure may shorten the distance between sampling and analysis. This distinction matters when researchers need timely information in remote, resource-limited, or rapidly changing settings.
A basic workflow begins with a biological sample from a clinical or environmental source, followed by exposure of its nucleic-acid targets to the PCR cycle. Primers bind the selected sequences, new DNA strands are extended, and repeated heating and cooling drives amplification. Fluorescence may then provide a readout of the amplification process, producing near-site molecular information.
In infection research, portable PCR units can be applied to pathogen detection in clinical samples, environmental surveillance, outbreak investigation, and diagnosis. The value is not limited to identifying a target in one specimen: placing testing closer to collection can support faster situational awareness when infections are being assessed across remote locations or during rapidly changing events.