Minimum Inhibitory Concentration
Minimum Inhibitory Concentration - Related Videos
05:16
A Microtiter Plate Method to Assess the Minimum Inhibitory Concentration of an Antibiotic
0 Views •
2025
This video demonstrates a microtiter plate-based method for determining the Minimum Inhibitory Concentration (MIC) of test antibiotics against bacteria. Upon adding a suspension of the bacterial pathogen Pseudomonas aeruginosa to a microtiter plate containing serial dilutions of a test antibiotic, and an inhibitor of the production of pyomelanin by the bacteria, the optical density of the wells is measured to compute the minimum inhibitory concentration of the antibiotic, and the impact of the...
01:31
Determining the Minimum Inhibitory Concentration of Enrofloxacin Against Avian Pathogenic E. coli
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2026
Begin with a suspension of avian pathogenic E. coli or APEC in growth media.APEC is a Gram-negative bacterium that infects poultry by entering through the respiratory tract.After breaching the mucosal layer, APEC adhere to epithelial cells, multiply locally, and disrupt the epithelial barrier to enter the bloodstream. The bacteria then spread to multiple organs, causing systemic infection.Add an equal volume of the bacterial suspension to each well of a multi-well plate containing increasing...
01:15
Determination of Multiple Dosing Parameters: Steady-State, Minimum and Maximum Concentrations
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2025
Gentamicin, an aminoglycoside antibiotic, is commonly administered via intermittent intravenous infusion to treat severe infections. An intermittent one-hour infusion of gentamicin, administered at eight-hour intervals, allows for precise control of plasma drug concentrations, minimizing toxicity while ensuring therapeutic efficacy. Pharmacokinetic principles govern the dynamics of plasma concentrations and can be mathematically described using specific equations.The plasma drug concentration...
03:02
Application of Dixon's Up-and-Down Design to Estimate the Minimum Alveolar Concentration of Sevoflurane in Rats with Refined Movement Classification
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2025
The protocol presented herein seeks to evaluate the depth of anesthesia utilizing Dixon's Up-and-Down Design in sedated rats subjected to a standardized pain stimulus. Furthermore, it delineates a classification of the various movement patterns observed.
09:50
Mapping Inhibitory Neuronal Circuits by Laser Scanning Photostimulation
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Cited by 6 •
2011
This paper introduces an approach of combining laser scanning photostimulation with whole cell recordings in transgenic mice expressing GFP in limited inhibitory neuron populations. The technique allows for extensive mapping and quantitative analysis of local synaptic circuits of specific inhibitory cortical neurons.
FAQs
Standardization makes the observed growth response attributable to the antimicrobial concentration rather than uncontrolled differences in the test system. The inoculum, drug dilutions, and laboratory conditions provide a consistent basis for identifying the first concentration associated with no detectable growth. This improves comparison of susceptibility results across experiments and supports meaningful interpretation in infection research.
An MIC becomes more clinically informative when compared with an established susceptibility breakpoint. That comparison places the measured response into an interpretation used to support treatment selection. The MIC itself reports the concentration associated with inhibited visible growth, whereas the breakpoint provides the framework for judging susceptibility and helping researchers evaluate whether an antimicrobial may be effective against the tested organism.
Changes in MIC values can help characterize shifts in pathogen susceptibility to an antimicrobial. When results are collected and compared over time, they contribute to surveillance of emerging drug resistance. This use extends beyond a single laboratory result: MIC data can help identify changing response patterns that matter for infection research, antimicrobial evaluation, and future treatment decisions.
A broth microdilution workflow begins by preparing serial concentrations of the antimicrobial and exposing each dilution to a standardized microbial inoculum. After incubation under defined laboratory conditions, the wells or samples are examined for detectable growth. The result is determined by locating the first concentration in the dilution series without visible growth, which supplies the MIC value.
Serial dilutions create an ordered range of antimicrobial concentrations for direct comparison against the same standardized microbial inoculum. After incubation, the pattern of growth across that range shows where inhibition begins. The first concentration without detectable growth is selected rather than an average or overall trend, allowing the assay to produce a specific susceptibility measurement.
MIC testing is useful when investigators need to characterize how infection-causing organisms respond to antibiotics or other antimicrobial agents. Results can support comparisons with susceptibility breakpoints, inform treatment selection, and assess candidate agents during antimicrobial development. Repeated measurements also contribute to monitoring emerging drug resistance, linking laboratory susceptibility data with broader infection-focused research.