Vaccine Immunogenicity
Vaccine Immunogenicity - Related Videos
05:42
Using a Sandwich ELISA to Determine the Immunogenic Glycoprotein Content in a Vaccine
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2025
This video showcases an indirect enzyme-linked immunosorbent assay or ELISA sandwich immunocapture assay designed to assess immunogenic glycoprotein levels in vaccines. The assay plate, containing glycoprotein-specific antibodies, is incubated with serially diluted vaccine samples, including reference and test vaccines, followed by immunoassay to quantify glycoprotein content in the test vaccine.
12:54
Determination of Vaccine Immunogenicity Using Bovine Monocyte-Derived Dendritic Cells
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Cited by 1 •
2023
The methodology describes the generation of bovine monocyte-derived dendritic cells (MoDCs) and their application for the in vitro evaluation of antigenic candidates during the development of potential veterinary vaccines in cattle.
01:51
Vaccinations
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2019
Overview Vaccination is the administration of antigenic material from pathogens to confer immunity against a specific microorganism. Vaccination primes the immune system to recognize and mount an immune response faster and more effectively if the real pathogen is encountered. Vaccinations are one of the most efficient ways to protect both individual humans and the general public from disease. A growing anti-vaccination skepticism risks the successes of vaccination programs that helped reduce...
06:17
Expression and Purification of Virus-like Particles for Vaccination
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Cited by 27 •
2016
Here, we present a protocol for synthesizing virus-like particles using either baculovirus or mammalian expression systems, and ultracentrifugation purification. This highly customizable approach is used to identify viral antigens as vaccine targets in a safe and flexible manner.
07:33
Intralymphatic Immunotherapy and Vaccination in Mice
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Cited by 18 •
2014
Prophylactic and therapeutic vaccination often fails to stimulate strong immune responses due to week drainage of the vaccine to lymph nodes and consequently poor involvement of immune cells. By direct injection of vaccine to lymph nodes, so-called intralymphatic injection, vaccine efficacy can be strongly improved and vaccine doses can be reduced.
FAQs
Antigen presentation exposes vaccine-derived antigen to lymphocytes, while innate immune signaling provides the activation context that helps initiate adaptive responses. Together, these processes influence whether vaccination produces antigen-specific antibodies, cellular immunity, and immune memory. Examining them helps immunologists connect early vaccine-triggered events with the quality of responses observed in infection-related studies.
Antibody measurements and cellular readouts capture different aspects of the adaptive response. Antibody titers quantify antigen-specific antibodies, while neutralization tests examine their functional activity. Cellular assays provide complementary information about immune-cell responses and coordination. Considering these results together gives researchers a broader view of how a vaccine engages immunity than relying on one measurement alone.
Vaccine formulation, dosing schedule, and the population receiving vaccination can all influence measured immune responses. Researchers compare these variables to determine whether one formulation or schedule produces stronger or more persistent antibody and cellular responses. Assessing durability is especially important because an initially detectable response may not represent the response maintained over time.
A typical evaluation combines several measurements rather than a single assay. Researchers may determine antibody titers, perform neutralization tests, examine cellular readouts, and conduct durability assessments. These results can be compared across vaccine formulations, dosing schedules, and populations. The combined data help characterize response magnitude, functional activity, cellular involvement, and persistence.
Researchers analyze antibody and cellular measurements across candidate formulations or schedules to identify differences in response quality and persistence. A comparison may include antibody titers, neutralization activity, cellular readouts, and follow-up durability assessments. This evidence supports selection of approaches that generate more useful or sustained immune responses and helps guide subsequent vaccine development.
Immunogenicity measurements help researchers evaluate how candidate vaccines respond to emerging pathogens and identify immune features associated with protection. Results can reveal potential correlates of protection, meaning measurable immune responses linked to protective effects. These findings inform vaccine design and contribute to public-health strategies by clarifying which responses should be monitored across populations.