Journal
/
Biology
/
Expression and Purification of Virus-like Particles for Vaccination
JoVE Journal
Biology
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Biology
Expression and Purification of Virus-like Particles for Vaccination

Expression and Purification of Virus-like Particles for Vaccination

DOI:
10.3791/54041-v

•

06:17 min

•

June 02, 2016

•

, ,
1Center for Vaccines and Immunology, Department of Infectious Diseases, College of Veterinary Medicine,University of Georgia

Chapters

  • 00:05Title
  • 00:58Influenza H3N2 Virus-like Particle (VLP) Generation
  • 02:10CHIK VLP Expression Using Baculovirus/Insect Cell System
  • 03:18Sedimentation and Purification of VLPs and SVPs
  • 04:42Results: Successful Expression and Purification of VLPs
  • 05:23Conclusion

Summary

Automatic Translation

Automatic Translation

Here, we present a protocol for synthesizing virus-like particles using either baculovirus or mammalian expression systems, and ultracentrifugation purification. This highly customizable approach is used to identify viral antigens as vaccine targets in a safe and flexible manner.

Tags

Keywords: Virus-like ParticlesVLPsVaccinationProtein ExpressionPurificationMammalian CellsInsect CellsTransfectionBaculovirusCHIKVAntigenVaccineSerologyDiagnosis

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

check_url/54041?article_type=v

The Preparation of Primary Hematopoietic Cell Cultures From Murine Bone Marrow for Electroporation
check_url/54041?article_type=v

Staining of Proteins in Gels with Coomassie G-250 without Organic Solvent and Acetic Acid
check_url/54041?article_type=v

Spinal Cord Electrophysiology
check_url/54041?article_type=v

Using the Gene Pulser MXcell Electroporation System to Transfect Primary Cells with High Efficiency
check_url/54041?article_type=v

Using an Automated Cell Counter to Simplify Gene Expression Studies: siRNA Knockdown of IL-4 Dependent Gene Expression in Namalwa Cells
check_url/54041?article_type=v

Expression, Detergent Solubilization, and Purification of a Membrane Transporter, the MexB Multidrug Resistance Protein
check_url/54041?article_type=v

Paraffin-Embedded and Frozen Sections of Drosophila Adult Muscles
check_url/54041?article_type=v

In vitro Reconstitution of the Active T. castaneum Telomerase
check_url/54041?article_type=v

Expression and Purification of the Cystic Fibrosis Transmembrane Conductance Regulator Protein in Saccharomyces cerevisiae
check_url/54041?article_type=v

Analysis of Global RNA Synthesis at the Single Cell Level following Hypoxia

Read Article