Chronic Lung Allograft
Chronic Lung Allograft - Related Videos
04:42
Picrosirius Red Staining for Semiquantitative Histopathologic Evaluation of Collagen Deposition in Murine Models of Chronic Lung Allograft Rejection
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Cited by 1 •
2025
Picrosirius Red staining is a semiquantitative method for objectively assessing collagen deposition in murine pulmonary fibrotic remodeling in a spatially resolved manner. Apart from evaluating total collagen deposition, Picrosirius Red staining allows for differentiating collagen fibers of different thicknesses.
10:01
Development of Obliterative Bronchiolitis in a Murine Model of Orthotopic Lung Transplantation
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Cited by 20 •
2012
Obliterative bronchiolitis is the key impediment to the long-term survival of lung transplant recipients and the lack of a robust preclinical model precludes examining obliterative bronchiolitis immunopathogenesis. Unlike other solid organ transplants, vascularized mouse lung transplantation has only recently been developed. Here we show our independently developed obliterative bronchiolitis model after murine orthotopic single-lung transplantation.
13:16
Computed Tomography and Optical Imaging of Osteogenesis-angiogenesis Coupling to Assess Integration of Cranial Bone Autografts and Allografts
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Cited by 7 •
2015
Implantation of autologous and allogeneic bone grafts constitute accepted approaches to treat major craniofacial bone loss. Yet the effect of graft composition on the interplay among neovascularization, cell differentiation and bone formation is unclear. We present a multimodal imaging protocol aimed to elucidate the angiogenesis-osteogenesis interdependence at the graft proximity.
09:14
Surgical Approach to Full Soft Tissue Face Allograft Procurement for Vascularized Composite Allotransplantation
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2025
This protocol details the surgical technique used to harvest and prepare a full-face allograft for transplantation in patients with severe facial disfigurements, focusing primarily on soft tissue procurement with limited bony harvest restricted to the nasal framework.
05:10
Intracranial Orthotopic Allografting of Medulloblastoma Cells in Immunocompromised Mice
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Cited by 5 •
2010
This protocol describes the isolation and dissociation of mouse medulloblastoma tissue, and subsequent allografting of the tumor cells into immunocompromised recipient mice in order to initiate secondary medulloblastoma.
FAQs
Persistent immune-mediated injury can damage both the small airways and the lung parenchyma, the functional tissue of the lung. As these structures sustain repeated inflammatory stress, allograft function may progressively decline. This mechanism is clinically important because recognizing immune injury as one possible contributor helps clinicians investigate chronic lung allograft dysfunction rather than attributing every change to a single cause.
Infection and aspiration are important because they represent inflammatory stresses that can injure transplanted lung tissue independently of immune-mediated damage. Their presence can complicate interpretation of worsening graft function and make it necessary to distinguish among several possible causes. Considering these contributors supports a more complete assessment of chronic lung allograft dysfunction and helps guide subsequent management.
Sustained graft health depends partly on immune tolerance, meaning the recipient’s immune system remains sufficiently compatible with the transplanted tissue to limit damaging responses. When tolerance is not maintained, immune-mediated injury may contribute to progressive loss of function. This relationship makes immune tolerance a central scientific focus in efforts to protect chronic lung allografts and extend graft survival.
Clinicians compare several types of evidence rather than relying on one finding. Pulmonary function and symptoms show how the patient is changing, imaging evaluates structural abnormalities, and biopsy findings provide tissue-level information. Interpreting these sources together can help distinguish rejection from infection, aspiration, or other inflammatory causes of decline, supporting more informed management of the allograft.
Post-transplant surveillance uses pulmonary function testing, symptom assessment, imaging, and biopsy findings to monitor graft health and identify potential complications. These approaches provide complementary information about functional change, clinical status, structural findings, and tissue injury. Their combined use helps clinicians detect chronic lung allograft dysfunction and evaluate the factors that may be contributing to deterioration.
Chronic lung allograft dysfunction represents progressive loss of transplanted lung function and therefore directly affects graft survival and patient outcomes. Studying it helps refine post-transplant surveillance, improve the distinction between rejection and other causes of decline, and develop strategies intended to preserve graft health. This research connects clinical monitoring with broader efforts to improve long-term transplant results.