Hot Plate Mixing

Hot plate mixing is a laboratory technique that combines controlled heating with mechanical stirring to dissolve, blend, or maintain uniform mixtures. A heated surface transfers thermal energy to a vessel while a magnetic stir bar rotates through the liquid, promoting convection, reducing concentration gradients, and supporting consistent temperature and composition. In medicine and pharmaceutical research, the method is used to prepare solutions, buffers, reagents, and formulation components under defined laboratory conditions. Careful control of temperature, mixing speed, vessel compatibility, and exposure time helps protect heat-sensitive materials and improves reproducibility in sample preparation, drug development, and clinical laboratory workflows.

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Education

JoVE Science Education - Advanced Biology

Pure Cultures and Streak Plating: Isolation of Single Bacterial Colonies from a Mixed Sample

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2023

Source: Tilde Andersson1, Rolf Lood1 1 Department of Clinical Sciences Lund, Division of Infection Medicine, Biomedical Center, Lund University, 221 00 Lund, Sweden Seemingly impossible to determine, microbial biodiversity is truly astounding with an estimated one trillion coexisting species (1,2). Although particularly harsh climates, like the acidic environment of the human stomach (3) or the subglacial lakes of Antarctica (4), may be dominated by a specific species, bacteria are typically...

Working with Hot and Cold Sources

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2023

Source: Robert M. Rioux & Suprita Jharimune, Pennsylvania State University, University Park, PA Working with extreme temperatures, both high and low, is an integral part of many laboratory operations. For many, mentioning a laboratory instantly evokes the mental picture of a Bunsen burner. Bunsen burners and hot plates are used extensively in small and large operations in research laboratories and industries, thus making it necessary for all users to be aware of their safe handling...

Hot Wire Anemometry

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2023

Source: Ricardo Mejia-Alvarez and Hussam Hikmat Jabbar, Department of Mechanical Engineering, Michigan State University, East Lansing, MI Hot-wire anemometers have a very short time-response, which makes them ideal to measure rapidly fluctuating phenomena such as turbulent flows. The purpose of this experiment is to demonstrate the use of hot-wire anemometry.

Hot Weather Concreting

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2024

Concreting at elevated temperatures accelerates the hydration process, leading to quicker setting but potentially reducing the long-term strength of the concrete structure. Additionally, low air humidity fosters rapid moisture loss from the concrete, resulting in reduced workability, pronounced plastic shrinkage, and a higher likelihood of crazing. Mitigating the heat increase in concrete can be economically achieved by shading aggregate stockpiles to prevent heating from solar radiation,...

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JoVE Journal - Biology
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Aseptic Laboratory Techniques: Plating Methods

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Cited by 438 •

2012

When working with media and reagents used to culture microorganisms, aseptic technique must be practiced to ensure contamination is minimized. A variety of plating methods are routinely used to isolate, propagate, or enumerate bacteria and phage, all of which incorporate procedures that maintain the sterility of experimental materials.

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