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Chapters
- 00:05Title
- 01:51A Safe and Sterile Workplace
- 03:04Streak Plate Procedure: Isolation of Bacterial Colonies using the Quadrant Method
- 05:26Pour Plate Procedure: Enumeration of Bacterial Cells in a Mixed Sample
- 06:38Spread-plating: Turntable with Glass or Metal Rod
- 07:59Spread-plating: the Copacabana Method
- 09:11Soft Agar Overlay Procedure: Plaque Assay
- 11:52Replica Plate Protocol: Screening Mutants and Auxotrophs
- 13:59Clean Up
- 14:25Practical Applications
- 17:22Conclusion
When working with media and reagents used to culture microorganisms, aseptic technique must be practiced to ensure contamination is minimized. A variety of plating methods are routinely used to isolate, propagate, or enumerate bacteria and phage, all of which incorporate procedures that maintain the sterility of experimental materials.
Tags
Aseptic Laboratory TechniquesPlating MethodsMicroorganismsContaminationSterilize Work SurfacesPrevent ContactSterile InstrumentsSterile SolutionsPlating MethodsIsolate MicroorganismsBacteriaPhageAseptic TechniqueStreak-platingPour-platingSpread-platingSoft Agar OverlaysIsolate PhageEnumerate PlaquesReplica-platingBiosafety Level 1 (BSL-1)Biosafety Level 2 (BSL-2)Biosafety CabinetBiohazard Classification
