Microfluidic Cartridge
Microfluidic Cartridge - Related Videos
03:31
A Microfluidic Cartridge-Based Detection System for Allergen-Specific Immunoglobulin E
0 Views •
2025
This video demonstrates a protocol for detecting serum-specific immunoglobulin E using a microfluidic cartridge-based chemiluminescence system. Serum IgEs interact with distinct allergens immobilized in the reaction zone of the cartridge, which are subsequently detected with peroxidase-conjugated anti-IgE antibodies and a chemiluminescent substrate, allowing simultaneous detection of multiple allergen-specific IgEs.
08:04
Use of a Filter Cartridge for Filtration of Water Samples and Extraction of Environmental DNA
0 Views •
Cited by 133 •
2016
We describe a protocol for filtration of water samples with a filter cartridge and extraction of environmental DNA (eDNA) without having to cut open the housing to remove the filter. This protocol is developed for metabarcoding eDNA from fishes, but is also applicable to eDNA from other organisms.
10:35
Studies of Bacterial Chemotaxis Using Microfluidics - Interview
0 Views •
Cited by 1 •
2007
10:18
Multi-step Variable Height Photolithography for Valved Multilayer Microfluidic Devices
0 Views •
Cited by 26 •
2017
Multilayer microfluidic devices often involve the fabrication of master molds with complex geometries for functionality. This article presents a complete protocol for multi-step photolithography with valves and variable height features tunable to any application. As a demonstration, we fabricate a microfluidic droplet generator capable of producing hydrogel beads.
11:16
A Microfluidic Chip for ICPMS Sample Introduction
0 Views •
Cited by 4 •
2015
We present a discrete droplet sample introduction system for inductively coupled plasma mass spectrometry (ICPMS). It is based on a cheap and disposable microfluidic chip that generates highly monodisperse droplets in a size range of 40−60 µm at frequencies from 90 to 7,000 Hz.
FAQs
Channel geometry determines how fluid is directed through the microscale network, while surface properties influence interactions between the sample and channel walls. Fluid forces then support controlled transport and can contribute to mixing, separation, or movement toward reaction and detection regions. These design features allow the cartridge to process small samples with limited reagents and maintain a controlled analytical workflow.
Integrated valves help regulate movement through different parts of the channel network, allowing sample handling and processing to occur in a planned sequence. Sensors and detection zones provide locations for measuring the processed sample. Combining these elements within one cartridge can connect preparation with analysis, reducing the need to transfer material between separate laboratory components.
The channel network can support several distinct operations, including transporting fluids, mixing components, separating sample constituents, and enabling reactions. The cartridge may combine these functions rather than treating them as isolated laboratory steps. This integration is important when sample volume and reagent availability are limited, because processing and measurement can occur within the same compact platform.
A typical workflow begins with introducing a clinical sample into the cartridge, followed by controlled movement through channels designed for preparation or processing. The sample may then pass through mixing, separation, or reaction regions before reaching a detection zone. Because preparation and measurement can be combined in one device, the overall workflow may require fewer handling steps and less material.
Medical applications include point-of-care diagnostics, blood and cell analysis, nucleic acid testing, and drug-response studies. The same compact format can support different analytical purposes by incorporating appropriate channel geometries, processing regions, sensors, or detection zones. These uses make the technology relevant both for near-patient testing and for studies that examine biological samples or treatment responses.
Their compact, often disposable format can reduce material use and combine sample preparation with measurement, helping shorten testing workflows. These features support testing near patients rather than requiring every step to occur in a centralized laboratory. They are also relevant in resource-limited settings, where conserving samples and reagents and simplifying handling can improve practical access to medical analysis.