Murine Vascular Model
Murine Vascular Model - Related Videos
08:24
Isolation of Murine Coronary Vascular Smooth Muscle Cells
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Cited by 11 •
2016
The purpose of this protocol is to demonstrate the isolation and culture techniques of murine primary vascular smooth muscle cells (VSMCs) from the coronary circulation. Once VSMCs have been isolated, they can be used for many standard culture techniques.
07:09
Isolation of Murine Adipose Tissue-derived Microvascular Fragments as Vascularization Units for Tissue Engineering
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Cited by 58 •
2017
We present a protocol to isolate adipose tissue-derived microvascular fragments that represent promising vascularization units. They can be rapidly isolated, do not require in vitro processing and, thus, may be used for one-step prevascularization in different fields of tissue engineering.
10:10
A Novel Murine Model of Arteriovenous Fistula Failure: The Surgical Procedure in Detail
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Cited by 24 •
2016
Here we present a murine model of arteriovenous fistula (AVF) failure in which a clinically relevant anastomotic configuration is incorporated. This model can be used to study the pathophysiology and to test possible therapeutic interventions.
08:54
Reliable Creation of a Murine Abdominal Aortic Aneurysm Model Using Elastin Sensitization and Perivascular Elastase
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2026
This protocol provides a repeatable and reliable method for inducing abdominal aortic aneurysms (AAAs) in C57BL/6 mice using elastin sensitization and perivascular elastase application, as an AAA model that incorporates an immune-sensitization component.
04:35
Murine Ileal Resection Model: Studying Ileectomy-Induced Bile Metabolism in Murine Model
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2023
This video describes the detailed protocol for intestinal resection in a murine model. This technique provides insights into ileectomy-induced bile malabsorption, overaccumulation, and toxicity in the mouse intestine.
FAQs
These components provide complementary indicators of vascular change. Endothelial cells reflect barrier and signaling functions, vascular smooth muscle contributes to vessel behavior, and remodeling shows structural adaptation over time. Examining them together helps distinguish altered permeability, impaired blood flow, and disease-related structural change rather than relying on a single vascular measurement.
Researchers can evaluate how each experimental condition changes vascular function and structure relative to appropriate comparisons. Genetic alterations may reveal the contribution of specific biological factors, disease stimuli model pathological pressure on vessels, and therapeutic interventions test whether those changes can be modified. This design connects a suspected mechanism with measurable physiological, imaging, or molecular outcomes.
Mice provide an intact living system for studying vascular responses, but similarities do not eliminate differences between murine and human biology. Those differences can affect how disease mechanisms, drug responses, or vascular remodeling appear experimentally. Researchers therefore use cross-species interpretation to judge which findings are clinically relevant and to design better translational studies.
Permeability describes how readily substances move across the vessel barrier, whereas tissue perfusion concerns the delivery of blood through tissues. A study that measures both can separate barrier dysfunction from inadequate flow. Including remodeling and molecular analyses adds structural and mechanistic context, helping investigators interpret whether an intervention improves function, architecture, or both.
A typical study establishes an experimental condition, introduces a disease-related stimulus or candidate intervention, and then evaluates vascular responses in the living organism. Investigators combine physiological measurements with imaging and molecular analyses, comparing the resulting changes across experimental conditions. The workflow links the intervention or alteration to vascular function, structure, and disease-related outcomes.
Physiological analysis assesses how vessels function, imaging examines vascular features in the living organism, and molecular analysis explores associated biological changes. Combining these approaches provides complementary evidence rather than treating one measurement as definitive. In medical research, this multimodal design can connect observable vascular behavior with cellular or molecular mechanisms relevant to disease.
They are useful when researchers need to examine cardiovascular disease, inflammation, tumor angiogenesis, or impaired tissue perfusion in a living system. The same platform can support evaluation of candidate drugs and procedures by showing how interventions affect vascular function and disease-related changes. Results also help guide the design of subsequent studies with clinical relevance.