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Isolation of Murine Coronary Vascular Smooth Muscle Cells
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Isolation of Murine Coronary Vascular Smooth Muscle Cells

Isolation of Murine Coronary Vascular Smooth Muscle Cells

DOI:
10.3791/53983-v

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08:24 min

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May 30, 2016

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, , , ,
1School of Biomedical Science,The Ohio State University College of Medicine, 2Center for Cardiovascular and Pulmonary Research,The Research Institute at Nationwide Children’s Hospital, 3Department of Pediatrics,The Ohio State University College of Medicine

Chapters

  • 00:05Title
  • 00:43Heart Isolation and Cannulation
  • 04:00Digestion and Vascular Smooth Muscle Cell (VSMC) Isolation
  • 06:08VSMC Culture
  • 07:10Results: Representative Cultured Coronary VSMC
  • 07:58Conclusion

Summary

Automatic Translation

Automatic Translation

The purpose of this protocol is to demonstrate the isolation and culture techniques of murine primary vascular smooth muscle cells (VSMCs) from the coronary circulation. Once VSMCs have been isolated, they can be used for many standard culture techniques.

Tags

Keywords: Murine Coronary Vascular Smooth Muscle CellsPrimary Coronary Vascular Smooth Muscle CellsCoronary CirculationCardiovascular FieldDisease MechanismsCell CultureVascular Smooth Muscle Cell BehaviorIsolationPerfusionCannulationHeparinHeart ExcisionAortaCell Culture Assays

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