Renal Vein Isolation

Renal vein isolation is a surgical technique that exposes and separates the renal vein from surrounding tissues to provide controlled access to the kidney’s venous drainage. Surgeons typically dissect along the vessel, identify tributaries and adjacent structures, and maintain direct visualization before applying vascular control or completing the planned procedure. This approach supports nephrectomy, renal transplantation, and other renal or retroperitoneal operations by improving anatomical orientation and helping manage venous bleeding. Careful isolation can also facilitate preservation of relevant vessels and reduce procedural risk, making it an important step in precise renal surgery.

Renal Vein Isolation - Related Videos

Research

JoVE Journal - Medicine
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Use of a Hanging-weight System for Isolated Renal Artery Occlusion

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Cited by 6 •

2011

A precise murine model for acute kidney injury (AKI) due to ischemia is an important tool to investigate acute kidney injury and possibly find therapeutic tools to treat renal injury. The hanging weight system offers a tool for immediate and reliable renal artery occlusion and reperfusion without causing renal congestion.

Research

JoVE EoE - Urinary Tract Cancer

Murine Renal Cell Isolation: A Technique to Isolate and Generate Primary Culture of Renal Tubular Epithelial Cells

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2023

In this video, we demonstrate the steps to isolate and purify renal tubular epithelial cells for a wide range of molecular biology and cell culture studies.

Murine Renal Transplantation Procedure

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Cited by 20 •

2009

Renal transplantation in mice is a technically challenging procedure that requires careful post-operative care and treatment for success.

Research

JoVE Journal - Medicine
Free Sample

Early Detection of Drug-Induced Renal Hemodynamic Dysfunction Using Sonographic Technology in Rats

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Cited by 14 •

2016

Early stage hemodynamic dysfunction is critical to the development of kidney disease. Yet, detection methodologies are limited. Recent advances in sonography provide a noninvasive, accurate option for early detection of kidney injury. This study outlines a step-by-step, sonographic methodology for detecting kidney dysfunction using a drug-induced nephrotoxicity rat model.

Isolation of Human Umbilical Vein Endothelial Cells (HUVEC)

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Cited by 131 •

2007

This video protocol illustrates the isolation and culture of human umbilical vein endothelial cells (HUVEC) from human umbilical cord. Once isolated these cells can be used for in vitro angiogenesis assays like the Optimized Fibrin Gel Bead Assay also demonstrated by the Hughes lab.

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